Polyvinylidene fluoride alters inflammatory responses by activation-induced cell death in macrophages

Hyun Gyung Kim, Sang Hoon Kim, Taek Seung Kim, Tae Won Park, Ran Won, Hee-Deung Park, Soo An Choi, Yong Woo Jung

Research output: Contribution to journalArticle

Abstract

Carbon nanotubes (CNTs) are nanomaterials that have been employed in generating diverse materials. We previously reported that CNTs induce cell death in macrophages, possibly via asbestosis. Therefore, we generated CNT-attached polyvinylidene fluoride (PVDF), which is an established polymer in membrane technology, and then examined whether CNT-attached PVDF is immunologically safe for medical purposes compared to CNT alone. To test this, we treated RAW 264.7 murine macrophages (RAW cells) with CNT-attached PVDF and analyzed the production of nitric oxide (NO), a potent proinflammatory mediator, in these cells. RAW cells treated with CNT-attached PVDF showed reduced NO production in response to lipopolysaccharide. However, the same treatment also decreased the cell number suggesting that this treatment can alter the homeostasis of RAW cells. Although cell cycle of RAW cells was increased by PVDF treatment with or without CNTs, apoptosis was enhanced in these cells. Taken together, these results indicate that PVDF with or without CNTs modulates inflammatory responses possibly due to activation-induced cell death in macrophages.

Original languageEnglish
Pages (from-to)402-409
Number of pages8
JournalImmune Network
Volume17
Issue number6
DOIs
Publication statusPublished - 2017 Dec 1

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Keywords

  • Carbon nanotubes
  • Cell death
  • Inflammation
  • Macrophages
  • Nitric oxide
  • Polyvinylidene fluoride

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Kim, H. G., Kim, S. H., Kim, T. S., Park, T. W., Won, R., Park, H-D., Choi, S. A., & Jung, Y. W. (2017). Polyvinylidene fluoride alters inflammatory responses by activation-induced cell death in macrophages. Immune Network, 17(6), 402-409. https://doi.org/10.4110/in.2017.17.6.402