Porcine testicular extract inhibits T cell proliferation by blocking cell cycle transition from G 1 phase to S phase

Han Hyoung Lee, Suk Jun Lee, Sunhee Kim, Seonah Jeong, Manheum Na, Dong Mok Lee, Yong Pil Cheon, Ki Ho Lee, Inho Choi, Taehoon Chun

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Since T cells express diverse sex steroid hormone receptors, they might be a good model to evaluate the effects of sex steroid hormones on immune modulation. Porcine testicular extract contains several sex steroid hormones and may be useful to study the effects of sex steroid hormones during T cell activation. We have examined the effects of the porcine testicular extract on T cell activation: proliferation and secretion of cytokines (IL-2 and IFN-γ) by activated T cells were severely decreased after treatment with porcine testicular extract. The extract produced an immunosuppressive effect and inhibited the proliferation of activated T cells by blocking the cell cycle transition from the G 1 phase to S phase. These effects were mediated by a decrease in the expression of cyclin D1 and cyclin E and constitutive expression of p27 KIP1 after T cell activation.

Original languageEnglish
Pages (from-to)1225-1233
Number of pages9
JournalBiotechnology letters
Volume34
Issue number7
DOIs
Publication statusPublished - 2012 Jul 1

    Fingerprint

Keywords

  • Cell cycle
  • Immunosuppressant
  • Porcine testicular extract
  • Steroid sex hormone
  • T cell activity

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Lee, H. H., Lee, S. J., Kim, S., Jeong, S., Na, M., Lee, D. M., Cheon, Y. P., Lee, K. H., Choi, I., & Chun, T. (2012). Porcine testicular extract inhibits T cell proliferation by blocking cell cycle transition from G 1 phase to S phase. Biotechnology letters, 34(7), 1225-1233. https://doi.org/10.1007/s10529-012-0907-6