Porous chitosan scaffold containing microspheres loaded with transforming growth factor-β1

Implications for cartilage tissue engineering

Sung Eun Kim, Jae Hyung Park, Yong Woo Cho, Hesson Chung, Seo Young Jeong, Eunhee Bae Lee, Ick Chan Kwon

Research output: Contribution to journalArticle

237 Citations (Scopus)

Abstract

Damaged articular cartilage, caused by traumatic injury or degenerative diseases, has a limited regenerative capacity and frequently leads to the onset of osteoarthritis. As a promising strategy for the successful regeneration of long-lasting hyaline cartilage, tissue engineering has received increasing recognition. In this study, we attempted to design a novel type of porous chitosan scaffold, containing transforming growth factor-β1 (TGF-β1), to enhance chondrogenesis. First, to achieve a sustained release of TGF-β1, chitosan microspheres loaded with TGF-β1 (MS-TGFs) were prepared by the emulsion method, in the presence of tripolyphosphate; with an identical manner, microspheres loaded with BSA, a model protein, were also prepared. Both microspheres containing TGF-β1and BSA had spherical shapes with a size ranging from 0.2 to 1.5 μm. From the release experiments, it was found that both proteins were slowly released from the microspheres over 5 days in a PBS solution (pH 7.4), in which the release rate of TGF-β1 was much lower than that of BSA. Second, MS-TGFs were seeded onto the porous chitosan scaffold, prepared by the freeze-drying method, to observe the effect on the proliferation and differentiation of chondrocytes. It was obviously demonstrated from in vitro tests that, compared to the scaffold without MS-TGF, the scaffold containing MS-TGF significantly augments the cell proliferation and production of extracellular matrix, indicating the role of TGF-β1 released from the microspheres. These results suggest that the chitosan scaffold containing MS-TGF possesses a promising potential as an implant to treat cartilage defects.

Original languageEnglish
Pages (from-to)365-374
Number of pages10
JournalJournal of Controlled Release
Volume91
Issue number3
DOIs
Publication statusPublished - 2003 Sep 4
Externally publishedYes

Fingerprint

Chitosan
Transforming Growth Factors
Tissue Engineering
Microspheres
Cartilage
Hyaline Cartilage
Chondrogenesis
Freeze Drying
Articular Cartilage
Chondrocytes
Emulsions
Osteoarthritis
Extracellular Matrix
Regeneration
Proteins
Cell Proliferation
Wounds and Injuries

Keywords

  • Articular cartilage
  • Chitosan
  • Chondrocyte
  • Microsphere
  • Sustained release
  • Transforming growth factor β1

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Porous chitosan scaffold containing microspheres loaded with transforming growth factor-β1 : Implications for cartilage tissue engineering. / Kim, Sung Eun; Park, Jae Hyung; Cho, Yong Woo; Chung, Hesson; Jeong, Seo Young; Lee, Eunhee Bae; Kwon, Ick Chan.

In: Journal of Controlled Release, Vol. 91, No. 3, 04.09.2003, p. 365-374.

Research output: Contribution to journalArticle

Kim, Sung Eun ; Park, Jae Hyung ; Cho, Yong Woo ; Chung, Hesson ; Jeong, Seo Young ; Lee, Eunhee Bae ; Kwon, Ick Chan. / Porous chitosan scaffold containing microspheres loaded with transforming growth factor-β1 : Implications for cartilage tissue engineering. In: Journal of Controlled Release. 2003 ; Vol. 91, No. 3. pp. 365-374.
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