Portable lysis apparatus for rapid single-step DNA extraction of Bacillus subtilis

H. J. Lim, E. H. Lee, Y. Yoon, Beelee Chua, Ahjeong Son

Research output: Contribution to journalArticle

Abstract

Aims: To demonstrate and characterize a portable lysis apparatus for rapid single-step bacterial DNA extraction. Methods and Results: Our portable lysis apparatus employed a novel design consisting of an annular piezo-element with perforated diaphragm. Using Bacillus subtilis as target bacteria, our portable lysis apparatus was able to achieve a normalized percent lysis as high as 66% within 30 s. This is comparable to that by microprobe ultrasonication and almost 7 times higher than that by conventional bead beating. The effect from adding glass beads was predictable. However, the results from the addition of sodium dodecyl sulphate (SDS) were counter-intuitive because a further increase from 0·5 to 1% concentration reduced the lysis performance. The portable lysis apparatus is also at least 1·5-5 times more power efficient than microprobe ultrasonication. Conclusions: Our portable lysis apparatus is capable of rapidly extracting bacterial DNA and is more power efficient than microprobe ultrasonication. The addition of glass beads or SDS concentration (up to 0·5%) improves its performance. Significance and Impact of the Study: The portable lysis apparatus provides a standalone, rapid, low cost and power efficient way of obtaining genomic constituents prior to a variety of bioassays used in the field of environmental, biomedical and other applied microbiology.

Original languageEnglish
Pages (from-to)379-387
Number of pages9
JournalJournal of Applied Microbiology
Volume120
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1

Fingerprint

Bacterial DNA
Bacillus subtilis
Sodium Dodecyl Sulfate
Glass
DNA
Microbiology
Diaphragm
Biological Assay
Bacteria
Costs and Cost Analysis

Keywords

  • Bacillus subtilis
  • Bacterial DNA extraction
  • Low cost
  • Portable lysis apparatus
  • Rapid

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology

Cite this

Portable lysis apparatus for rapid single-step DNA extraction of Bacillus subtilis. / Lim, H. J.; Lee, E. H.; Yoon, Y.; Chua, Beelee; Son, Ahjeong.

In: Journal of Applied Microbiology, Vol. 120, No. 2, 01.02.2016, p. 379-387.

Research output: Contribution to journalArticle

Lim, H. J. ; Lee, E. H. ; Yoon, Y. ; Chua, Beelee ; Son, Ahjeong. / Portable lysis apparatus for rapid single-step DNA extraction of Bacillus subtilis. In: Journal of Applied Microbiology. 2016 ; Vol. 120, No. 2. pp. 379-387.
@article{9bc135c388af4bf3850763b3ed8af6cd,
title = "Portable lysis apparatus for rapid single-step DNA extraction of Bacillus subtilis",
abstract = "Aims: To demonstrate and characterize a portable lysis apparatus for rapid single-step bacterial DNA extraction. Methods and Results: Our portable lysis apparatus employed a novel design consisting of an annular piezo-element with perforated diaphragm. Using Bacillus subtilis as target bacteria, our portable lysis apparatus was able to achieve a normalized percent lysis as high as 66{\%} within 30 s. This is comparable to that by microprobe ultrasonication and almost 7 times higher than that by conventional bead beating. The effect from adding glass beads was predictable. However, the results from the addition of sodium dodecyl sulphate (SDS) were counter-intuitive because a further increase from 0·5 to 1{\%} concentration reduced the lysis performance. The portable lysis apparatus is also at least 1·5-5 times more power efficient than microprobe ultrasonication. Conclusions: Our portable lysis apparatus is capable of rapidly extracting bacterial DNA and is more power efficient than microprobe ultrasonication. The addition of glass beads or SDS concentration (up to 0·5{\%}) improves its performance. Significance and Impact of the Study: The portable lysis apparatus provides a standalone, rapid, low cost and power efficient way of obtaining genomic constituents prior to a variety of bioassays used in the field of environmental, biomedical and other applied microbiology.",
keywords = "Bacillus subtilis, Bacterial DNA extraction, Low cost, Portable lysis apparatus, Rapid",
author = "Lim, {H. J.} and Lee, {E. H.} and Y. Yoon and Beelee Chua and Ahjeong Son",
year = "2016",
month = "2",
day = "1",
doi = "10.1111/jam.13011",
language = "English",
volume = "120",
pages = "379--387",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "2",

}

TY - JOUR

T1 - Portable lysis apparatus for rapid single-step DNA extraction of Bacillus subtilis

AU - Lim, H. J.

AU - Lee, E. H.

AU - Yoon, Y.

AU - Chua, Beelee

AU - Son, Ahjeong

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Aims: To demonstrate and characterize a portable lysis apparatus for rapid single-step bacterial DNA extraction. Methods and Results: Our portable lysis apparatus employed a novel design consisting of an annular piezo-element with perforated diaphragm. Using Bacillus subtilis as target bacteria, our portable lysis apparatus was able to achieve a normalized percent lysis as high as 66% within 30 s. This is comparable to that by microprobe ultrasonication and almost 7 times higher than that by conventional bead beating. The effect from adding glass beads was predictable. However, the results from the addition of sodium dodecyl sulphate (SDS) were counter-intuitive because a further increase from 0·5 to 1% concentration reduced the lysis performance. The portable lysis apparatus is also at least 1·5-5 times more power efficient than microprobe ultrasonication. Conclusions: Our portable lysis apparatus is capable of rapidly extracting bacterial DNA and is more power efficient than microprobe ultrasonication. The addition of glass beads or SDS concentration (up to 0·5%) improves its performance. Significance and Impact of the Study: The portable lysis apparatus provides a standalone, rapid, low cost and power efficient way of obtaining genomic constituents prior to a variety of bioassays used in the field of environmental, biomedical and other applied microbiology.

AB - Aims: To demonstrate and characterize a portable lysis apparatus for rapid single-step bacterial DNA extraction. Methods and Results: Our portable lysis apparatus employed a novel design consisting of an annular piezo-element with perforated diaphragm. Using Bacillus subtilis as target bacteria, our portable lysis apparatus was able to achieve a normalized percent lysis as high as 66% within 30 s. This is comparable to that by microprobe ultrasonication and almost 7 times higher than that by conventional bead beating. The effect from adding glass beads was predictable. However, the results from the addition of sodium dodecyl sulphate (SDS) were counter-intuitive because a further increase from 0·5 to 1% concentration reduced the lysis performance. The portable lysis apparatus is also at least 1·5-5 times more power efficient than microprobe ultrasonication. Conclusions: Our portable lysis apparatus is capable of rapidly extracting bacterial DNA and is more power efficient than microprobe ultrasonication. The addition of glass beads or SDS concentration (up to 0·5%) improves its performance. Significance and Impact of the Study: The portable lysis apparatus provides a standalone, rapid, low cost and power efficient way of obtaining genomic constituents prior to a variety of bioassays used in the field of environmental, biomedical and other applied microbiology.

KW - Bacillus subtilis

KW - Bacterial DNA extraction

KW - Low cost

KW - Portable lysis apparatus

KW - Rapid

UR - http://www.scopus.com/inward/record.url?scp=84955286038&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84955286038&partnerID=8YFLogxK

U2 - 10.1111/jam.13011

DO - 10.1111/jam.13011

M3 - Article

VL - 120

SP - 379

EP - 387

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 2

ER -