Portable lysis apparatus for rapid single-step DNA extraction of Bacillus subtilis

H. J. Lim, E. H. Lee, Y. Yoon, Beelee Chua, Ahjeong Son

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Aims: To demonstrate and characterize a portable lysis apparatus for rapid single-step bacterial DNA extraction. Methods and Results: Our portable lysis apparatus employed a novel design consisting of an annular piezo-element with perforated diaphragm. Using Bacillus subtilis as target bacteria, our portable lysis apparatus was able to achieve a normalized percent lysis as high as 66% within 30 s. This is comparable to that by microprobe ultrasonication and almost 7 times higher than that by conventional bead beating. The effect from adding glass beads was predictable. However, the results from the addition of sodium dodecyl sulphate (SDS) were counter-intuitive because a further increase from 0·5 to 1% concentration reduced the lysis performance. The portable lysis apparatus is also at least 1·5-5 times more power efficient than microprobe ultrasonication. Conclusions: Our portable lysis apparatus is capable of rapidly extracting bacterial DNA and is more power efficient than microprobe ultrasonication. The addition of glass beads or SDS concentration (up to 0·5%) improves its performance. Significance and Impact of the Study: The portable lysis apparatus provides a standalone, rapid, low cost and power efficient way of obtaining genomic constituents prior to a variety of bioassays used in the field of environmental, biomedical and other applied microbiology.

Original languageEnglish
Pages (from-to)379-387
Number of pages9
JournalJournal of Applied Microbiology
Volume120
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1

Keywords

  • Bacillus subtilis
  • Bacterial DNA extraction
  • Low cost
  • Portable lysis apparatus
  • Rapid

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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