Possible role of amyloid β-(1-40)-BSA conjugates in transdifferentiation of lens epithelial cells

Kwang Won Lee, Young Seomun, Dong Hwan Kim, Sun Young Park, Choun K. Joo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We investigated whether amyloid β (Aβ) aggregates have transforming growth factor β- like cytokine activity and cause transdifferention of lens epithelial cells, leading to certain types of cataract. In order to mimic Aβ aggregates, Aβ-(1-40) was crosslinked to bovine serum albumin (BSA) with disuccinimidyl suberate according to a previously described procedure. When human lens epithelial B-3 (HLE B-3) cells were treated with the Aβ-(1-40)-BSA conjugates, we observed the translocation of Smad-3, as well as the induced mRNA levels of fibronectin (FN), collagen type I (Col I), smooth muscle actin (SMA) and matrix metalloproteinase-2 (MAP-2). In addition, we investigated the morphology of rat whole lens cultured for 5 days in the presence of Aβ-(1-40)-BSA, and the immunohistochemical localizations of Aβ-(1-40)/amyloid precursor protein (APP) in human clinical tissues beneath the anterior capsules. In rat whole lens cultures, treatment with A β-(1-40)-BSA produced a transformed morphology that had multiple layers of lens epithelial cells. To compare the anterior capsules in anterior subcapsular cataracts with those in nuclear cataracts, immunohistochemical studies of Aβ/APP in human clinical tissues revealed that the predominant immunostaining of Aβ occurs in the anterior epithelial plaques, which likely produces the abnormal extracellular matrix. Thus, these findings suggest that Aβ aggregates in vivo are possibly involved in the regulatory process by which lens epithelial cells may transdifferentiate into fibroblast-like cells, as well as help understand the mechanisms which lead to certain types of cataractogenesis.

Original languageEnglish
Pages (from-to)219-228
Number of pages10
JournalYonsei Medical Journal
Volume45
Issue number2
Publication statusPublished - 2004 Apr 30

Fingerprint

Bovine Serum Albumin
Amyloid
Lenses
Epithelial Cells
Cataract
Serum Amyloid A Protein
Amyloid beta-Protein Precursor
Capsules
Matrix Metalloproteinase 2
Transforming Growth Factors
Collagen Type I
Fibronectins
Extracellular Matrix
Smooth Muscle
Actins
Fibroblasts
Cytokines
Messenger RNA

Keywords

  • Amyloid
  • Cataractogenesis
  • Epithelial cells
  • Extra-cellular matrix
  • Growth factors
  • Lens differentiation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Possible role of amyloid β-(1-40)-BSA conjugates in transdifferentiation of lens epithelial cells. / Lee, Kwang Won; Seomun, Young; Kim, Dong Hwan; Park, Sun Young; Joo, Choun K.

In: Yonsei Medical Journal, Vol. 45, No. 2, 30.04.2004, p. 219-228.

Research output: Contribution to journalArticle

Lee, Kwang Won ; Seomun, Young ; Kim, Dong Hwan ; Park, Sun Young ; Joo, Choun K. / Possible role of amyloid β-(1-40)-BSA conjugates in transdifferentiation of lens epithelial cells. In: Yonsei Medical Journal. 2004 ; Vol. 45, No. 2. pp. 219-228.
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