Post-transcriptional regulation of the xynA expression by a novel mRNA binding protein, XaiF

Mi Young Jeong, Eung Ryoung Lee, Cheol-Won Yun, Ssang G. Cho, Yong J. Choi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

XaiF, a novel 32 kDa protein encoded by the ORF located in the immediate downstream of the xynA gene of Bacillus stearothermophilus No. 236, was identified to be the xylanase-specific trans-activator. In this study, the positive effect of XaiF was confirmed to be xylanase-specific, and the results from Northern blot and in vitro transcription assays showed that the XaiF increased the xynA transcripts at post-transcriptional step. Moreover, analysis of the mRNA decay rate led to the assertion that the XaiF functions to stabilize the xynA mRNA. Intriguingly, in vitro RNA-protein binding assay and analysis using gst-xynA 3′-UTR chimeric gene constructs demonstrated that the XaiF stabilizes xynA mRNA by direct binding onto the 3′-UTR of the mRNA.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume351
Issue number1
DOIs
Publication statusPublished - 2006 Dec 8

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Keywords

  • 3′-Untranslational region
  • Bacillus stearothermophilus No. 236
  • mRNA binding protein
  • mRNA stability
  • Post-transcriptional regulation
  • xaiF
  • Xylanase
  • xynA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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