Post-translational regulation of SHORT VEGETATIVE PHASE as a major mechanism for thermoregulation of flowering

Jeong Hwan Lee, Kyung Sook Chung, Soon Kap Kim, Ji Hoon Ahn

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In contrast to our extensive knowledge of vernalization, we know relatively little about the regulation of ambient temperature-responsive flowering. Recent reports revealed that FLOWERING LOCUS M (FLM) and SHORT VEGETATIVE PHASE (SVP) regulate high ambient temperature-responsive flowering through two different mechanisms: degradation of SVP protein and formation of a non-functional SVP-FLM-δ complex. To investigate further the mechanism of thermoregulation of flowering, we performed real-time quantitative polymerase chain reaction (RT -qPCR) and in vitro pull-down assays. We found that FLM-β and FLM-δ transcripts show similar absolute levels at different temperatures. Also, His-SVP protein bound to the GST-FLM-β or -δ proteins with similar binding intensities. These results suggest that functional SVP-FLM-β and non-functional SVP-FLM-δ complexes form similarly at warmer temperatures, thus indicating that posttranslational regulation of SVP functions as a major mechanism for thermoregulation in flowering.

Original languageEnglish
Article numbere28193
JournalPlant Signaling and Behavior
Volume9
Issue numberMAR
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

thermoregulation
flowering
ambient temperature
proteins
vernalization
temperature
quantitative polymerase chain reaction
degradation
assays

Keywords

  • Alternative splicing
  • Ambient temperature
  • Ambient temperature-responsive flowering
  • FLM-β
  • FLM-δ
  • SVP
  • Thermoregulation

ASJC Scopus subject areas

  • Plant Science

Cite this

Post-translational regulation of SHORT VEGETATIVE PHASE as a major mechanism for thermoregulation of flowering. / Lee, Jeong Hwan; Chung, Kyung Sook; Kim, Soon Kap; Ahn, Ji Hoon.

In: Plant Signaling and Behavior, Vol. 9, No. MAR, e28193, 01.01.2014.

Research output: Contribution to journalArticle

@article{dd890e05d6694beaa51031f3013a6917,
title = "Post-translational regulation of SHORT VEGETATIVE PHASE as a major mechanism for thermoregulation of flowering",
abstract = "In contrast to our extensive knowledge of vernalization, we know relatively little about the regulation of ambient temperature-responsive flowering. Recent reports revealed that FLOWERING LOCUS M (FLM) and SHORT VEGETATIVE PHASE (SVP) regulate high ambient temperature-responsive flowering through two different mechanisms: degradation of SVP protein and formation of a non-functional SVP-FLM-δ complex. To investigate further the mechanism of thermoregulation of flowering, we performed real-time quantitative polymerase chain reaction (RT -qPCR) and in vitro pull-down assays. We found that FLM-β and FLM-δ transcripts show similar absolute levels at different temperatures. Also, His-SVP protein bound to the GST-FLM-β or -δ proteins with similar binding intensities. These results suggest that functional SVP-FLM-β and non-functional SVP-FLM-δ complexes form similarly at warmer temperatures, thus indicating that posttranslational regulation of SVP functions as a major mechanism for thermoregulation in flowering.",
keywords = "Alternative splicing, Ambient temperature, Ambient temperature-responsive flowering, FLM-β, FLM-δ, SVP, Thermoregulation",
author = "Lee, {Jeong Hwan} and Chung, {Kyung Sook} and Kim, {Soon Kap} and Ahn, {Ji Hoon}",
year = "2014",
month = "1",
day = "1",
doi = "10.4161/psb.28193",
language = "English",
volume = "9",
journal = "Plant Signaling and Behavior",
issn = "1559-2316",
publisher = "Landes Bioscience",
number = "MAR",

}

TY - JOUR

T1 - Post-translational regulation of SHORT VEGETATIVE PHASE as a major mechanism for thermoregulation of flowering

AU - Lee, Jeong Hwan

AU - Chung, Kyung Sook

AU - Kim, Soon Kap

AU - Ahn, Ji Hoon

PY - 2014/1/1

Y1 - 2014/1/1

N2 - In contrast to our extensive knowledge of vernalization, we know relatively little about the regulation of ambient temperature-responsive flowering. Recent reports revealed that FLOWERING LOCUS M (FLM) and SHORT VEGETATIVE PHASE (SVP) regulate high ambient temperature-responsive flowering through two different mechanisms: degradation of SVP protein and formation of a non-functional SVP-FLM-δ complex. To investigate further the mechanism of thermoregulation of flowering, we performed real-time quantitative polymerase chain reaction (RT -qPCR) and in vitro pull-down assays. We found that FLM-β and FLM-δ transcripts show similar absolute levels at different temperatures. Also, His-SVP protein bound to the GST-FLM-β or -δ proteins with similar binding intensities. These results suggest that functional SVP-FLM-β and non-functional SVP-FLM-δ complexes form similarly at warmer temperatures, thus indicating that posttranslational regulation of SVP functions as a major mechanism for thermoregulation in flowering.

AB - In contrast to our extensive knowledge of vernalization, we know relatively little about the regulation of ambient temperature-responsive flowering. Recent reports revealed that FLOWERING LOCUS M (FLM) and SHORT VEGETATIVE PHASE (SVP) regulate high ambient temperature-responsive flowering through two different mechanisms: degradation of SVP protein and formation of a non-functional SVP-FLM-δ complex. To investigate further the mechanism of thermoregulation of flowering, we performed real-time quantitative polymerase chain reaction (RT -qPCR) and in vitro pull-down assays. We found that FLM-β and FLM-δ transcripts show similar absolute levels at different temperatures. Also, His-SVP protein bound to the GST-FLM-β or -δ proteins with similar binding intensities. These results suggest that functional SVP-FLM-β and non-functional SVP-FLM-δ complexes form similarly at warmer temperatures, thus indicating that posttranslational regulation of SVP functions as a major mechanism for thermoregulation in flowering.

KW - Alternative splicing

KW - Ambient temperature

KW - Ambient temperature-responsive flowering

KW - FLM-β

KW - FLM-δ

KW - SVP

KW - Thermoregulation

UR - http://www.scopus.com/inward/record.url?scp=84899135743&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899135743&partnerID=8YFLogxK

U2 - 10.4161/psb.28193

DO - 10.4161/psb.28193

M3 - Article

C2 - 25764420

AN - SCOPUS:84899135743

VL - 9

JO - Plant Signaling and Behavior

JF - Plant Signaling and Behavior

SN - 1559-2316

IS - MAR

M1 - e28193

ER -