Postharvest disease control efficacy of the polyene macrolide lucensomycin produced by Streptomyces plumbeus strain CA5 against gray mold on grapes

Jeong Do Kim, Ji Eun Kang, Beom Seok Kim

Research output: Contribution to journalArticle

Abstract

Microbial secondary metabolites have been a valuable source of antifungal compounds for the fungicide industry to develop plant disease control agents. In order to find natural antifungal compounds useful for the control of Botrytis cinerea causing postharvest gray mold, we screened microbial culture extracts and found the extract of Streptomyces plumbeus strain CA5 which was isolated from the soil sample of Chuncheon Province. The cell extract of CA5 strain markedly reduced the disease incidence of gray mold on grapes to a value of 22.2 % while the non-treated control showed 100 % disease incidence. The active ingredient (CA5A) was identified from the cell extract of CA5 strain using a variety of chromatographic methods and spectroscopic analyses. The structure of CA5A was determined to be the polyene macrolide lucensomycin. Lucensomycin showed inhibitory effects against the spores of B. cinerea at a concentration as low as 1 mg L−1 and completely inhibited gray mold development on grapes at a concentration of 100 mg L−1.

Original languageEnglish
Article number111115
JournalPostharvest Biology and Technology
Volume162
DOIs
Publication statusPublished - 2020 Apr

Fingerprint

Lucensomycin
Polyenes
macrolides
postharvest diseases
Streptomyces
Macrolides
Vitis
grapes
disease control
Fungi
Cell Extracts
extracts
Botrytis cinerea
disease incidence
Botrytis
Plant Diseases
Incidence
Spores
Industry
Soil

Keywords

  • Antagonistic actinomycete
  • Biofungicides
  • Gray mold
  • Lucensomycin
  • Polyene macrolide
  • Postharvest disease

ASJC Scopus subject areas

  • Food Science
  • Agronomy and Crop Science
  • Horticulture

Cite this

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title = "Postharvest disease control efficacy of the polyene macrolide lucensomycin produced by Streptomyces plumbeus strain CA5 against gray mold on grapes",
abstract = "Microbial secondary metabolites have been a valuable source of antifungal compounds for the fungicide industry to develop plant disease control agents. In order to find natural antifungal compounds useful for the control of Botrytis cinerea causing postharvest gray mold, we screened microbial culture extracts and found the extract of Streptomyces plumbeus strain CA5 which was isolated from the soil sample of Chuncheon Province. The cell extract of CA5 strain markedly reduced the disease incidence of gray mold on grapes to a value of 22.2 {\%} while the non-treated control showed 100 {\%} disease incidence. The active ingredient (CA5A) was identified from the cell extract of CA5 strain using a variety of chromatographic methods and spectroscopic analyses. The structure of CA5A was determined to be the polyene macrolide lucensomycin. Lucensomycin showed inhibitory effects against the spores of B. cinerea at a concentration as low as 1 mg L−1 and completely inhibited gray mold development on grapes at a concentration of 100 mg L−1.",
keywords = "Antagonistic actinomycete, Biofungicides, Gray mold, Lucensomycin, Polyene macrolide, Postharvest disease",
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AU - Kang, Ji Eun

AU - Kim, Beom Seok

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N2 - Microbial secondary metabolites have been a valuable source of antifungal compounds for the fungicide industry to develop plant disease control agents. In order to find natural antifungal compounds useful for the control of Botrytis cinerea causing postharvest gray mold, we screened microbial culture extracts and found the extract of Streptomyces plumbeus strain CA5 which was isolated from the soil sample of Chuncheon Province. The cell extract of CA5 strain markedly reduced the disease incidence of gray mold on grapes to a value of 22.2 % while the non-treated control showed 100 % disease incidence. The active ingredient (CA5A) was identified from the cell extract of CA5 strain using a variety of chromatographic methods and spectroscopic analyses. The structure of CA5A was determined to be the polyene macrolide lucensomycin. Lucensomycin showed inhibitory effects against the spores of B. cinerea at a concentration as low as 1 mg L−1 and completely inhibited gray mold development on grapes at a concentration of 100 mg L−1.

AB - Microbial secondary metabolites have been a valuable source of antifungal compounds for the fungicide industry to develop plant disease control agents. In order to find natural antifungal compounds useful for the control of Botrytis cinerea causing postharvest gray mold, we screened microbial culture extracts and found the extract of Streptomyces plumbeus strain CA5 which was isolated from the soil sample of Chuncheon Province. The cell extract of CA5 strain markedly reduced the disease incidence of gray mold on grapes to a value of 22.2 % while the non-treated control showed 100 % disease incidence. The active ingredient (CA5A) was identified from the cell extract of CA5 strain using a variety of chromatographic methods and spectroscopic analyses. The structure of CA5A was determined to be the polyene macrolide lucensomycin. Lucensomycin showed inhibitory effects against the spores of B. cinerea at a concentration as low as 1 mg L−1 and completely inhibited gray mold development on grapes at a concentration of 100 mg L−1.

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