Postnatal treadmill exercise alleviates prenatal stress-induced anxiety in offspring rats by enhancing cell proliferation through 5-hydroxytryptamine 1A receptor activation

Sam Jun Lee, Tae Woon Kim, Hun Kyung Park, Sangyun Yoon, Ann Hee You, Eun Jin Moon, Dong Hoon Shin, Han Jin Cho

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Purpose: Stress during pregnancy is a risk factor for the development of anxiety-related disorders in offspring later in life. The effects of treadmill exercise on anxiety-like behaviors and hippocampal cell proliferation were investigated using rats exposed to prenatal stress. Methods: Exposure of pregnant rats to a hunting dog in an enclosed room was used to induce stress. Anxiety-like behaviors of offspring were evaluated using the elevated plus maze test. Immunohistochemistry for the detection of 5-bromo-2'-deoxyuridine and doublecortin (DCX) in the hippocampal dentate gyrus and 5-hydroxytryptamine 1A receptors (5-HT1A) in the dorsal raphe was conducted. Brain-derived neurotrophic factor (BDNF) and tyrosine kinase B (TrkB) levels in the hippocampus were evaluated by western blot analysis. Results: Offspring of maternal rats exposed to stress during pregnancy showed anxiety-like behaviors. Offspring also showed reduced expression of BDNF, TrkB, and DCX in the dentate gyrus, decreased cell proliferation in the hippocampus, and reduced 5-HT1A expression in the dorsal raphe. Postnatal treadmill exercise by offspring, but not maternal exercise during pregnancy, enhanced cell proliferation and expression of these proteins. Conclusions: Postnatal treadmill exercise ameliorated anxiety-like behaviors in offspring of stressed pregnant rats, and the alleviating effect of exercise on these behaviors is hypothesized to result from enhancement of cell proliferation through 5-HT1A activation in offspring rats.

Original languageEnglish
Pages (from-to)S57-S64
JournalInternational Neurourology Journal
Volume20
DOIs
Publication statusPublished - 2016 May 1

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Receptor, Serotonin, 5-HT1A
Anxiety
Cell Proliferation
Brain-Derived Neurotrophic Factor
Dentate Gyrus
Pregnancy
Protein-Tyrosine Kinases
Hippocampus
Mothers
Parahippocampal Gyrus
Bromodeoxyuridine
Anxiety Disorders
Western Blotting
Immunohistochemistry
Dogs
Proteins

Keywords

  • Anxiety
  • Cell proliferation
  • Exercise test
  • Prenatal exposure delayed effects
  • Serotonin

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Urology

Cite this

Postnatal treadmill exercise alleviates prenatal stress-induced anxiety in offspring rats by enhancing cell proliferation through 5-hydroxytryptamine 1A receptor activation. / Lee, Sam Jun; Kim, Tae Woon; Park, Hun Kyung; Yoon, Sangyun; You, Ann Hee; Moon, Eun Jin; Shin, Dong Hoon; Cho, Han Jin.

In: International Neurourology Journal, Vol. 20, 01.05.2016, p. S57-S64.

Research output: Contribution to journalArticle

Lee, Sam Jun ; Kim, Tae Woon ; Park, Hun Kyung ; Yoon, Sangyun ; You, Ann Hee ; Moon, Eun Jin ; Shin, Dong Hoon ; Cho, Han Jin. / Postnatal treadmill exercise alleviates prenatal stress-induced anxiety in offspring rats by enhancing cell proliferation through 5-hydroxytryptamine 1A receptor activation. In: International Neurourology Journal. 2016 ; Vol. 20. pp. S57-S64.
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