Postneonatal mortality and liver changes in cloned pigs associated with human tumor necrosis factor receptor I-Fc and human heme oxygenase-1 overexpression

Geon A. Kim, Jun Xue Jin, Sanghoon Lee, Anukul Taweechaipaisankul, Hyun Ju Oh, Jong-Ik Hwang, Curie Ahn, Islam M. Saadeldin, Byeong Chun Lee

Research output: Contribution to journalArticle

Abstract

Soluble human tumor necrosis factor (shTNFRI-Fc) and human heme oxygenase 1 (hHO-1) are key regulators for protection against oxidative and inflammatory injury for xenotransplantation. Somatic cells with more than 10 copy numbers of shTNFRI-Fc and hHO-1 were employed in somatic cell nuclear transfer to generate cloned pigs, thereby resulting in seven cloned piglets. However, produced piglets were all dead within 24 hours after birth. Obviously, postnatal death with liver apoptosis was reported in the higher copy number of shTNFRI-Fc and hHO-1 piglets. In liver, the transcript levels of ferritin heavy chain, light chain, transferrin, and inducible nitric oxide synthase were significantly highly expressed compared to those of lower copy number of shTNFRI-Fc and hHO-1 piglets (P<0.05). Also, H2O2 contents were increased, and superoxide dismutase was significantly lower in the higher copy number of shTNFRI-Fc and hHO-1 piglets (P<0.05). These results indicate that TNFRI-Fc and hHO-1 overexpression may apparently induce free iron in the liver and exert oxidative stress by enhancing reactive oxygen species production and block normal postneonatal liver metabolism.

Original languageEnglish
Article number5276576
JournalBioMed Research International
Volume2017
DOIs
Publication statusPublished - 2017 Jan 1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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