Pre-concentration without changes in ph in an open reservoir utilizing ion concentration polarization

Youngkyu Cho, David Wonbin Lim, Junghyo Yoon, Jaeho Kim, Jeong Hoon Lee, Seok Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Pre-concentration of liquid sample without pH change is essential for diagnosis in patient samples with low concentration virus or biological molecules. Target molecule's isoelectric point (PI) value and optimal pH should be stable in antibody-antigen reaction [1], to facilitate robust and accurate detection. Previously, pre-concentration using a buffer drain technique is developed for concentrating protein. Previous technique has a problem of changing pH of sample when utilizing electric field in the sample reservoir [2]. Here, we developed a simple micro-fluidic device, which can concentrate particle without changes in pH, based PDMS utilizing ion concentration polarization (ICP) by ion selective layer made of Nafion [3].

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1205-1207
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

Keywords

  • Ion concentration polarization
  • Microfluidic
  • Pre-concentration

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Cho, Y., Lim, D. W., Yoon, J., Kim, J., Lee, J. H., & Chung, S. (2015). Pre-concentration without changes in ph in an open reservoir utilizing ion concentration polarization. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1205-1207). Chemical and Biological Microsystems Society.