Abstract
Pre-concentration of liquid sample without pH change is essential for diagnosis in patient samples with low concentration virus or biological molecules. Target molecule's isoelectric point (PI) value and optimal pH should be stable in antibody-antigen reaction [1], to facilitate robust and accurate detection. Previously, pre-concentration using a buffer drain technique is developed for concentrating protein. Previous technique has a problem of changing pH of sample when utilizing electric field in the sample reservoir [2]. Here, we developed a simple micro-fluidic device, which can concentrate particle without changes in pH, based PDMS utilizing ion concentration polarization (ICP) by ion selective layer made of Nafion [3].
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1205-1207 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| Publication status | Published - 2015 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 2015 Oct 25 → 2015 Oct 29 |
Other
| Other | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country | Korea, Republic of |
| City | Gyeongju |
| Period | 15/10/25 → 15/10/29 |
Keywords
- Ion concentration polarization
- Microfluidic
- Pre-concentration
ASJC Scopus subject areas
- Control and Systems Engineering
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Cite this
Cho, Y., Lim, D. W., Yoon, J., Kim, J., Lee, J. H., & Chung, S. (2015). Pre-concentration without changes in ph in an open reservoir utilizing ion concentration polarization. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1205-1207). Chemical and Biological Microsystems Society.