Abstract
Pre-concentration of liquid sample without pH change is essential for diagnosis in patient samples with low concentration virus or biological molecules. Target molecule's isoelectric point (PI) value and optimal pH should be stable in antibody-antigen reaction [1], to facilitate robust and accurate detection. Previously, pre-concentration using a buffer drain technique is developed for concentrating protein. Previous technique has a problem of changing pH of sample when utilizing electric field in the sample reservoir [2]. Here, we developed a simple micro-fluidic device, which can concentrate particle without changes in pH, based PDMS utilizing ion concentration polarization (ICP) by ion selective layer made of Nafion [3].
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1205-1207 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| Publication status | Published - 2015 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 2015 Oct 25 → 2015 Oct 29 |
Other
| Other | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country | Korea, Republic of |
| City | Gyeongju |
| Period | 15/10/25 → 15/10/29 |
Fingerprint
Keywords
- Ion concentration polarization
- Microfluidic
- Pre-concentration
ASJC Scopus subject areas
- Control and Systems Engineering
Cite this
Pre-concentration without changes in ph in an open reservoir utilizing ion concentration polarization. / Cho, Youngkyu; Lim, David Wonbin; Yoon, Junghyo; Kim, Jaeho; Lee, Jeong Hoon; Chung, Seok.
MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 1205-1207.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Pre-concentration without changes in ph in an open reservoir utilizing ion concentration polarization
AU - Cho, Youngkyu
AU - Lim, David Wonbin
AU - Yoon, Junghyo
AU - Kim, Jaeho
AU - Lee, Jeong Hoon
AU - Chung, Seok
PY - 2015
Y1 - 2015
N2 - Pre-concentration of liquid sample without pH change is essential for diagnosis in patient samples with low concentration virus or biological molecules. Target molecule's isoelectric point (PI) value and optimal pH should be stable in antibody-antigen reaction [1], to facilitate robust and accurate detection. Previously, pre-concentration using a buffer drain technique is developed for concentrating protein. Previous technique has a problem of changing pH of sample when utilizing electric field in the sample reservoir [2]. Here, we developed a simple micro-fluidic device, which can concentrate particle without changes in pH, based PDMS utilizing ion concentration polarization (ICP) by ion selective layer made of Nafion [3].
AB - Pre-concentration of liquid sample without pH change is essential for diagnosis in patient samples with low concentration virus or biological molecules. Target molecule's isoelectric point (PI) value and optimal pH should be stable in antibody-antigen reaction [1], to facilitate robust and accurate detection. Previously, pre-concentration using a buffer drain technique is developed for concentrating protein. Previous technique has a problem of changing pH of sample when utilizing electric field in the sample reservoir [2]. Here, we developed a simple micro-fluidic device, which can concentrate particle without changes in pH, based PDMS utilizing ion concentration polarization (ICP) by ion selective layer made of Nafion [3].
KW - Ion concentration polarization
KW - Microfluidic
KW - Pre-concentration
UR - http://www.scopus.com/inward/record.url?scp=84983243410&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84983243410&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84983243410
SP - 1205
EP - 1207
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
ER -