Predominance of cluster I Clostridium in hydrogen fermentation of galactose seeded with various heat-treated anaerobic sludges

Jeong Hoon Park, Sang Hoon Lee, Jeong Jun Yoon, Sang Hyoun Kim, Hee-Deung Park

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

To identify the key bacterial populations in hydrogen fermentation of galactose, a fermentor seeded with a heat-treated sludge was operated. After 27h of fermentation, the proportion of butyric acid increased to 69.4wt.% and the gas production yield reached 1.0molH2/molgalactose. In the pyrosequencing of 16S rDNA, an increase of the proportion of the phylum Firmicutes from 4.2% to 92% (mostly cluster I Clostridium) was observed. To verify the predominance and the ubiquity of the cluster, five fermentors seeded with different heat-treated anaerobic sludges having different feedstock compositions and digestion temperatures were investigated using qPCR analyses. The abundance of the cluster increased >100-fold during the fermentation, regardless of the inocula. Moreover, the abundance was negatively correlated with the lag time of hydrogen production and positively correlated with the hydrogen production rate, demonstrating the relevance of the cluster to hydrogen production. Taken together, the results clearly revealed the importance of cluster I Clostridium in the hydrogen fermentation of galactose.

Original languageEnglish
Pages (from-to)98-106
Number of pages9
JournalBioresource Technology
Volume157
DOIs
Publication statusPublished - 2014 Apr 1

Fingerprint

Clostridium
Galactose
Fermentation
fermentation
Hydrogen
Hydrogen production
sludge
hydrogen
Butyric acid
Butyric Acid
Ribosomal DNA
Feedstocks
gas production
Gases
digestion
Hot Temperature
fold
Chemical analysis
acid
Temperature

Keywords

  • Biohydrogen
  • Clostridium
  • Galactose
  • Marine red algae
  • Pyrosequencing

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Predominance of cluster I Clostridium in hydrogen fermentation of galactose seeded with various heat-treated anaerobic sludges. / Park, Jeong Hoon; Lee, Sang Hoon; Yoon, Jeong Jun; Kim, Sang Hyoun; Park, Hee-Deung.

In: Bioresource Technology, Vol. 157, 01.04.2014, p. 98-106.

Research output: Contribution to journalArticle

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