Preferential nucleosome assembly at DNA triplet repeats from the myotonic dystrophy gene

Yuh Hwa Wang; Sorour Amirhaeri; Seongman Kang; Robert D. Wells; Jack D. Griffith

doi:10.1126/science.8036515

Preferential nucleosome assembly at DNA triplet repeats from the myotonic dystrophy gene

Yuh Hwa Wang, Sorour Amirhaeri, Seongman Kang, Robert D. Wells, Jack D. Griffith

Research output: Contribution to journal › Article › peer-review

211 Citations (Scopus)

Abstract

The expansion of CTG repeats in DNA occurs in or near genes involved in several human diseases, including myotonic dystrophy and Huntington's disease. Nucleosomes, the basic structural element of chromosomes, consist of 146 base pairs of DNA coiled about an octamer of histone proteins and mediate general transcriptional repression. Electron microscopy was used to examine in vitro the nucleosome assembly of DNA containing repeating CTG triplets. The efficiency of nucleosome formation increased with expanded triplet blocks, suggesting that such blocks may repress transcription through the creation of stable nucleosomes.

Original language	English
Pages (from-to)	669-671
Number of pages	3
Journal	Science
Volume	265
Issue number	5172
DOIs	https://doi.org/10.1126/science.8036515
Publication status	Published - 1994
Externally published	Yes

ASJC Scopus subject areas

General

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1126/science.8036515

Cite this

@article{d658f5a80adb4d20b24b2c308c05ac8c,

title = "Preferential nucleosome assembly at DNA triplet repeats from the myotonic dystrophy gene",

abstract = "The expansion of CTG repeats in DNA occurs in or near genes involved in several human diseases, including myotonic dystrophy and Huntington's disease. Nucleosomes, the basic structural element of chromosomes, consist of 146 base pairs of DNA coiled about an octamer of histone proteins and mediate general transcriptional repression. Electron microscopy was used to examine in vitro the nucleosome assembly of DNA containing repeating CTG triplets. The efficiency of nucleosome formation increased with expanded triplet blocks, suggesting that such blocks may repress transcription through the creation of stable nucleosomes.",

author = "Wang, {Yuh Hwa} and Sorour Amirhaeri and Seongman Kang and Wells, {Robert D.} and Griffith, {Jack D.}",

year = "1994",

doi = "10.1126/science.8036515",

language = "English",

volume = "265",

pages = "669--671",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5172",

}

TY - JOUR

T1 - Preferential nucleosome assembly at DNA triplet repeats from the myotonic dystrophy gene

AU - Wang, Yuh Hwa

AU - Amirhaeri, Sorour

AU - Kang, Seongman

AU - Wells, Robert D.

AU - Griffith, Jack D.

PY - 1994

Y1 - 1994

N2 - The expansion of CTG repeats in DNA occurs in or near genes involved in several human diseases, including myotonic dystrophy and Huntington's disease. Nucleosomes, the basic structural element of chromosomes, consist of 146 base pairs of DNA coiled about an octamer of histone proteins and mediate general transcriptional repression. Electron microscopy was used to examine in vitro the nucleosome assembly of DNA containing repeating CTG triplets. The efficiency of nucleosome formation increased with expanded triplet blocks, suggesting that such blocks may repress transcription through the creation of stable nucleosomes.

AB - The expansion of CTG repeats in DNA occurs in or near genes involved in several human diseases, including myotonic dystrophy and Huntington's disease. Nucleosomes, the basic structural element of chromosomes, consist of 146 base pairs of DNA coiled about an octamer of histone proteins and mediate general transcriptional repression. Electron microscopy was used to examine in vitro the nucleosome assembly of DNA containing repeating CTG triplets. The efficiency of nucleosome formation increased with expanded triplet blocks, suggesting that such blocks may repress transcription through the creation of stable nucleosomes.

UR - http://www.scopus.com/inward/record.url?scp=0027941198&partnerID=8YFLogxK

U2 - 10.1126/science.8036515

DO - 10.1126/science.8036515

M3 - Article

C2 - 8036515

AN - SCOPUS:0027941198

VL - 265

SP - 669

EP - 671

JO - Science

JF - Science

SN - 0036-8075

IS - 5172

ER -

Preferential nucleosome assembly at DNA triplet repeats from the myotonic dystrophy gene

Abstract

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this