Preferential nucleosome assembly at DNA triplet repeats from the myotonic dystrophy gene

Yuh Hwa Wang, Sorour Amirhaeri, Seong Man Kang, Robert D. Wells, Jack D. Griffith

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202 Citations (Scopus)

Abstract

The expansion of CTG repeats in DNA occurs in or near genes involved in several human diseases, including myotonic dystrophy and Huntington's disease. Nucleosomes, the basic structural element of chromosomes, consist of 146 base pairs of DNA coiled about an octamer of histone proteins and mediate general transcriptional repression. Electron microscopy was used to examine in vitro the nucleosome assembly of DNA containing repeating CTG triplets. The efficiency of nucleosome formation increased with expanded triplet blocks, suggesting that such blocks may repress transcription through the creation of stable nucleosomes.

Original languageEnglish
Pages (from-to)669-671
Number of pages3
JournalScience
Volume265
Issue number5172
Publication statusPublished - 1994 Jul 29
Externally publishedYes

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Cite this

Wang, Y. H., Amirhaeri, S., Kang, S. M., Wells, R. D., & Griffith, J. D. (1994). Preferential nucleosome assembly at DNA triplet repeats from the myotonic dystrophy gene. Science, 265(5172), 669-671.