Preferential nucleosome assembly at DNA triplet repeats from the myotonic dystrophy gene

Yuh Hwa Wang; Sorour Amirhaeri; Seongman Kang; Robert D. Wells; Jack D. Griffith

doi:10.1126/science.8036515

Preferential nucleosome assembly at DNA triplet repeats from the myotonic dystrophy gene

Yuh Hwa Wang, Sorour Amirhaeri, Seongman Kang, Robert D. Wells, Jack D. Griffith

Research output: Contribution to journal › Article › peer-review

203 Citations (Scopus)

Abstract

The expansion of CTG repeats in DNA occurs in or near genes involved in several human diseases, including myotonic dystrophy and Huntington's disease. Nucleosomes, the basic structural element of chromosomes, consist of 146 base pairs of DNA coiled about an octamer of histone proteins and mediate general transcriptional repression. Electron microscopy was used to examine in vitro the nucleosome assembly of DNA containing repeating CTG triplets. The efficiency of nucleosome formation increased with expanded triplet blocks, suggesting that such blocks may repress transcription through the creation of stable nucleosomes.

Original language	English
Pages (from-to)	669-671
Number of pages	3
Journal	Science
Volume	265
Issue number	5172
DOIs	https://doi.org/10.1126/science.8036515
Publication status	Published - 1994
Externally published	Yes

ASJC Scopus subject areas

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AU - Kang, Seongman

AU - Wells, Robert D.

AU - Griffith, Jack D.

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AB - The expansion of CTG repeats in DNA occurs in or near genes involved in several human diseases, including myotonic dystrophy and Huntington's disease. Nucleosomes, the basic structural element of chromosomes, consist of 146 base pairs of DNA coiled about an octamer of histone proteins and mediate general transcriptional repression. Electron microscopy was used to examine in vitro the nucleosome assembly of DNA containing repeating CTG triplets. The efficiency of nucleosome formation increased with expanded triplet blocks, suggesting that such blocks may repress transcription through the creation of stable nucleosomes.

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