Pregnancy and interferon tau regulate N-myc interactor in the ovine uterus

Gwonhwa Song, J. A G W Fleming, J. Kim, T. E. Spencer, F. W. Bazer

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In ruminants, interferon tau (IFNT) is synthesized and secreted by the mononuclear trophectoderm cells of the conceptus and maintains the corpus luteum and its secretion of progesterone for successful implantation and maintenance of pregnancy. In this study, we examined regulation of the expression of N-myc interactor (NMI) gene by IFNT in the ovine uterus based on results of microarray data from a study that compared gene expression by human 2fTGH and U3A (STAT1-null 2fTGH) cell lines in response to treatment with IFNT or vehicle. In the present study, semiquantitative reverse transcription-polymerase chain reaction analyses verified that IFNT stimulated expression of NMI mRNA in 2fTGH (ie, in a STAT1-dependent manner), but not in U3A (STAT1-null) cells. Furthermore, results of western blot analyses indicated that immunoreactive NMI proteins in 2fTGH and U3A cell lines increased in a time-dependent manner only in response to IFNT. In ovine endometria, steady-state levels of NMI mRNA increased between days 14 and 16 of pregnancy and then decreased slightly by day 20, but there was no effect of day of the estrous cycle. Expression of NMI mRNA was most abundant in endometrial stromal cells, glandular epithelium, and conceptus trophectoderm. Intrauterine infusion of IFNT in cyclic ewes increased expression of NMI in the endometrium. Expression of NMI in ovine and bovine uterine cell lines increased in response to IFNT. Collectively, the results of the present study indicate that IFNT regulates expression of NMI mRNA and protein in ovine endometria during pregnancy via a STAT1-dependent cell signaling pathway.

Original languageEnglish
Pages (from-to)87-97
Number of pages11
JournalDomestic Animal Endocrinology
Volume40
Issue number2
DOIs
Publication statusPublished - 2011 Feb 1
Externally publishedYes

Fingerprint

interferons
uterus
Uterus
Sheep
pregnancy
sheep
Pregnancy
endometrium
Endometrium
Null Lymphocytes
Messenger RNA
conceptus
cell lines
Cell Line
Pregnancy Maintenance
Proto-Oncogene Proteins c-myc
intrauterine administration
myc Genes
Estrous Cycle
trophoblastin

Keywords

  • Interferon tau
  • N-myc interactor
  • Pregnancy
  • Uterus

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology
  • Food Animals

Cite this

Pregnancy and interferon tau regulate N-myc interactor in the ovine uterus. / Song, Gwonhwa; Fleming, J. A G W; Kim, J.; Spencer, T. E.; Bazer, F. W.

In: Domestic Animal Endocrinology, Vol. 40, No. 2, 01.02.2011, p. 87-97.

Research output: Contribution to journalArticle

Song, Gwonhwa ; Fleming, J. A G W ; Kim, J. ; Spencer, T. E. ; Bazer, F. W. / Pregnancy and interferon tau regulate N-myc interactor in the ovine uterus. In: Domestic Animal Endocrinology. 2011 ; Vol. 40, No. 2. pp. 87-97.
@article{3470bc0baa414e11887909cdc815e227,
title = "Pregnancy and interferon tau regulate N-myc interactor in the ovine uterus",
abstract = "In ruminants, interferon tau (IFNT) is synthesized and secreted by the mononuclear trophectoderm cells of the conceptus and maintains the corpus luteum and its secretion of progesterone for successful implantation and maintenance of pregnancy. In this study, we examined regulation of the expression of N-myc interactor (NMI) gene by IFNT in the ovine uterus based on results of microarray data from a study that compared gene expression by human 2fTGH and U3A (STAT1-null 2fTGH) cell lines in response to treatment with IFNT or vehicle. In the present study, semiquantitative reverse transcription-polymerase chain reaction analyses verified that IFNT stimulated expression of NMI mRNA in 2fTGH (ie, in a STAT1-dependent manner), but not in U3A (STAT1-null) cells. Furthermore, results of western blot analyses indicated that immunoreactive NMI proteins in 2fTGH and U3A cell lines increased in a time-dependent manner only in response to IFNT. In ovine endometria, steady-state levels of NMI mRNA increased between days 14 and 16 of pregnancy and then decreased slightly by day 20, but there was no effect of day of the estrous cycle. Expression of NMI mRNA was most abundant in endometrial stromal cells, glandular epithelium, and conceptus trophectoderm. Intrauterine infusion of IFNT in cyclic ewes increased expression of NMI in the endometrium. Expression of NMI in ovine and bovine uterine cell lines increased in response to IFNT. Collectively, the results of the present study indicate that IFNT regulates expression of NMI mRNA and protein in ovine endometria during pregnancy via a STAT1-dependent cell signaling pathway.",
keywords = "Interferon tau, N-myc interactor, Pregnancy, Uterus",
author = "Gwonhwa Song and Fleming, {J. A G W} and J. Kim and Spencer, {T. E.} and Bazer, {F. W.}",
year = "2011",
month = "2",
day = "1",
doi = "10.1016/j.domaniend.2010.09.003",
language = "English",
volume = "40",
pages = "87--97",
journal = "Domestic Animal Endocrinology",
issn = "0739-7240",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Pregnancy and interferon tau regulate N-myc interactor in the ovine uterus

AU - Song, Gwonhwa

AU - Fleming, J. A G W

AU - Kim, J.

AU - Spencer, T. E.

AU - Bazer, F. W.

PY - 2011/2/1

Y1 - 2011/2/1

N2 - In ruminants, interferon tau (IFNT) is synthesized and secreted by the mononuclear trophectoderm cells of the conceptus and maintains the corpus luteum and its secretion of progesterone for successful implantation and maintenance of pregnancy. In this study, we examined regulation of the expression of N-myc interactor (NMI) gene by IFNT in the ovine uterus based on results of microarray data from a study that compared gene expression by human 2fTGH and U3A (STAT1-null 2fTGH) cell lines in response to treatment with IFNT or vehicle. In the present study, semiquantitative reverse transcription-polymerase chain reaction analyses verified that IFNT stimulated expression of NMI mRNA in 2fTGH (ie, in a STAT1-dependent manner), but not in U3A (STAT1-null) cells. Furthermore, results of western blot analyses indicated that immunoreactive NMI proteins in 2fTGH and U3A cell lines increased in a time-dependent manner only in response to IFNT. In ovine endometria, steady-state levels of NMI mRNA increased between days 14 and 16 of pregnancy and then decreased slightly by day 20, but there was no effect of day of the estrous cycle. Expression of NMI mRNA was most abundant in endometrial stromal cells, glandular epithelium, and conceptus trophectoderm. Intrauterine infusion of IFNT in cyclic ewes increased expression of NMI in the endometrium. Expression of NMI in ovine and bovine uterine cell lines increased in response to IFNT. Collectively, the results of the present study indicate that IFNT regulates expression of NMI mRNA and protein in ovine endometria during pregnancy via a STAT1-dependent cell signaling pathway.

AB - In ruminants, interferon tau (IFNT) is synthesized and secreted by the mononuclear trophectoderm cells of the conceptus and maintains the corpus luteum and its secretion of progesterone for successful implantation and maintenance of pregnancy. In this study, we examined regulation of the expression of N-myc interactor (NMI) gene by IFNT in the ovine uterus based on results of microarray data from a study that compared gene expression by human 2fTGH and U3A (STAT1-null 2fTGH) cell lines in response to treatment with IFNT or vehicle. In the present study, semiquantitative reverse transcription-polymerase chain reaction analyses verified that IFNT stimulated expression of NMI mRNA in 2fTGH (ie, in a STAT1-dependent manner), but not in U3A (STAT1-null) cells. Furthermore, results of western blot analyses indicated that immunoreactive NMI proteins in 2fTGH and U3A cell lines increased in a time-dependent manner only in response to IFNT. In ovine endometria, steady-state levels of NMI mRNA increased between days 14 and 16 of pregnancy and then decreased slightly by day 20, but there was no effect of day of the estrous cycle. Expression of NMI mRNA was most abundant in endometrial stromal cells, glandular epithelium, and conceptus trophectoderm. Intrauterine infusion of IFNT in cyclic ewes increased expression of NMI in the endometrium. Expression of NMI in ovine and bovine uterine cell lines increased in response to IFNT. Collectively, the results of the present study indicate that IFNT regulates expression of NMI mRNA and protein in ovine endometria during pregnancy via a STAT1-dependent cell signaling pathway.

KW - Interferon tau

KW - N-myc interactor

KW - Pregnancy

KW - Uterus

UR - http://www.scopus.com/inward/record.url?scp=78650714884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650714884&partnerID=8YFLogxK

U2 - 10.1016/j.domaniend.2010.09.003

DO - 10.1016/j.domaniend.2010.09.003

M3 - Article

C2 - 21067885

AN - SCOPUS:78650714884

VL - 40

SP - 87

EP - 97

JO - Domestic Animal Endocrinology

JF - Domestic Animal Endocrinology

SN - 0739-7240

IS - 2

ER -