Preparation and analysis of yeast cell wall mannoproteins, immune enchancing materials, from cell wall mutant Saccharomyces cerevisiae

Chang Hoon Ha, Cheol-Won Yun, Hyun Dong Paik, Seung Wook Kim, Chang Won Kang, Han Joon Hwang, Hyo-Ihl Chang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Yeast cell wall matrix particles are composed entirely of mannoprotein and β-glucan. The mannoproteins of yeast cell wall can systemically enhance the immune system. We previously purified and analyzed alkali-soluble β-glucans [β-(1,3)- and β-(1,6)-glucans] [10]. In the present study, a wild-type strain was first mutagenized with ultraviolet light, and the cell wall mutants were then selected by treatment with 1.0 mg/ml laminarinase (endo-β-(1,3)-D-glucanase). Mannoproteins of Saccharomyces cerevisiae were released by laminarinase, purified by concanavalin-A affinity and ion-exchange chromatography. The results indicated that the mutants yielded 3-fold more mannoprotein than the wild-type. The mannoprotein mass of mutant K48L3 was 2.25 mg/100 mg of yeast cell dry mass. Carbohydrate analysis revealed that they contained mannose, glucose, and N-acetylglucosamine. Saccharomyces cerevisiae cell wall components, mannoproteins, are known to interact with macrophages through receptors, thereby inducing release of tumor necrosis factor alpha (TNF-α) and nitric oxide. Mannoprotein fractions in the present study had a higher macrophage activity of secretion of TNF-α and nitric oxide and direct phagocytosis than positive control (1 μg of lipopolysaccharide). In particular, F1 and F3 fractions in mannoproteins of K48L3 enhanced and upregulated the activity of nitric oxide secretion and macrophage phagocytosis by approximately two- and four-fold, respectively.

Original languageEnglish
Pages (from-to)247-255
Number of pages9
JournalJournal of Microbiology and Biotechnology
Volume16
Issue number2
Publication statusPublished - 2006 Feb 1

Fingerprint

Yeast
Cell Wall
Saccharomyces cerevisiae
Yeasts
Cells
Macrophages
Nitric oxide
Cellulases
Nitric Oxide
Glucans
Phagocytosis
Immune system
Carbohydrates
Chromatography
Tumor Necrosis Factor-alpha
Glucose
Ion exchange
Acetylglucosamine
mannoproteins
Ion Exchange Chromatography

Keywords

  • Immune activity
  • Mannoprotein
  • Random mutation
  • Yeast cell wall

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Preparation and analysis of yeast cell wall mannoproteins, immune enchancing materials, from cell wall mutant Saccharomyces cerevisiae. / Ha, Chang Hoon; Yun, Cheol-Won; Paik, Hyun Dong; Kim, Seung Wook; Kang, Chang Won; Hwang, Han Joon; Chang, Hyo-Ihl.

In: Journal of Microbiology and Biotechnology, Vol. 16, No. 2, 01.02.2006, p. 247-255.

Research output: Contribution to journalArticle

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