Preparation and analysis of yeast cell wall mannoproteins, immune enchancing materials, from cell wall mutant Saccharomyces cerevisiae

Chang Hoon Ha, Cheol Won Yun, Hyun Dong Paik, Seung Wook Kim, Chang Won Kang, Han Joon Hwang, Hyo Ihl Chang

Research output: Contribution to journalReview article

6 Citations (Scopus)

Abstract

Yeast cell wall matrix particles are composed entirely of mannoprotein and β-glucan. The mannoproteins of yeast cell wall can systemically enhance the immune system. We previously purified and analyzed alkali-soluble β-glucans [β-(1,3)- and β-(1,6)-glucans] [10]. In the present study, a wild-type strain was first mutagenized with ultraviolet light, and the cell wall mutants were then selected by treatment with 1.0 mg/ml laminarinase (endo-β-(1,3)-D-glucanase). Mannoproteins of Saccharomyces cerevisiae were released by laminarinase, purified by concanavalin-A affinity and ion-exchange chromatography. The results indicated that the mutants yielded 3-fold more mannoprotein than the wild-type. The mannoprotein mass of mutant K48L3 was 2.25 mg/100 mg of yeast cell dry mass. Carbohydrate analysis revealed that they contained mannose, glucose, and N-acetylglucosamine. Saccharomyces cerevisiae cell wall components, mannoproteins, are known to interact with macrophages through receptors, thereby inducing release of tumor necrosis factor alpha (TNF-α) and nitric oxide. Mannoprotein fractions in the present study had a higher macrophage activity of secretion of TNF-α and nitric oxide and direct phagocytosis than positive control (1 μg of lipopolysaccharide). In particular, F1 and F3 fractions in mannoproteins of K48L3 enhanced and upregulated the activity of nitric oxide secretion and macrophage phagocytosis by approximately two- and four-fold, respectively.

Original languageEnglish
Pages (from-to)247-255
Number of pages9
JournalJournal of microbiology and biotechnology
Volume16
Issue number2
Publication statusPublished - 2006 Feb

Keywords

  • Immune activity
  • Mannoprotein
  • Random mutation
  • Yeast cell wall

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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