Preparation and biodegradation of thermosensitive chitosan hydrogel as a function of pH and temperature

Dong Han Hee, Eun Nam Da, Hoan Seo Dong, Woo Kim Tae, Cheol Shin Byung, Suk Choi Ho

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)


We have developed an injectable thermosensitive hydrogel for local drug delivery to treat cancers clinically. We selected chitosan as a polymer matrix because of its biocompatibility and biodegradability. Glycerol 2-phosphate disodium salt hydrate (β-GP) was used to neutralize the chitosan solution to physiological pH. The chitosan solution displayed a sol-gel phase transition in a pH- and temperature-dependent manner and formed an endothermic hydrogel after subcutaneous injection into mouse in the presence of β-GP. Additionally, we evaluated the biodegradation of chitosan hydrogel in mice by measuring the volume of injected chitosan hydrogel after subcutaneous injection. The injected chitosan hydrogel in mice was sected and stained with hematoxylin-eosin reagent for histological observation to confirm biodegradation of the hydrogel by the infiltrated cells. Chitosan hydrogel systems that possess biocompatibility and biodegradability could be promising thermosensitive injectable materials useful as depot systems for local anti-cancer drug delivery.

Original languageEnglish
Pages (from-to)507-511
Number of pages5
JournalMacromolecular Research
Issue number5
Publication statusPublished - 2004 Oct
Externally publishedYes


  • Biodegradation
  • Chitosan hydrogel
  • Sol-gel transition

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry


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