Preparation and characterization of cisplatin-incorporated chitosan hydrogels, microparticles, and nanoparticles

Jueun Cha, Bum Lee Won, Rae Park Chong, Woo Cho Yong, Cheol Hee Ahn, Ick Chan Kwon

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Three different, polymer-platinum conjugates (hydrogels, microparticles, and nanoparticles) were synthesized by complexation of cis- dichlorodiammineplatinum(II) (cisplatin) with partially succinylated glycol chitosan (PSGC). Succinic anhydride was used as a linker to introduce cisplatin to glycol chitosan (GC). Succinylation of GC was investigated systematically as a function of the molar ratio of succinic anhydride to glucosamine, the methanol content in the reaction media, and the reaction temperature. By controlling the reaction conditions, water-soluble, partially water-soluble, and hydrogel-forming PSGCs were synthesized, and then conjugated with cisplatin. The complexation of cisplatin with water-soluble PSGC via a ligand exchange reaction of platinum from chloride to the carboxylates induced the formation of nano-sized aggregates in aqueous media. The hydrodynamic diameters of PSGC/cisplatin complex nano-aggregates, as determined by light scattering, were 180-300 nm and the critical aggregation concentrations (CACs), as determined by a fluorescence technique using pyrene as a probe, were 20-30 μg/mL. The conjugation of cisplatin with partially water-soluble PSGC, i.e., borderline between water-soluble and water-insoluble PSGC, produced micro-sized particles <500 μm. Cisplatin-complexed PSGC hydrogels were prepared from water-insoluble PSGCs. All of the cisplatin-incorporated, polymer matrices released platinum in a sustained manner without any significant initial burst, suggesting that they may all be useful as slow release systems for cisplatin. The release rate of platinum increased with the morphology changes from hydrogel through microparticle to nanoparticle systems.

Original languageEnglish
Pages (from-to)573-578
Number of pages6
JournalMacromolecular Research
Volume14
Issue number5
Publication statusPublished - 2006 Oct 1
Externally publishedYes

Fingerprint

Hydrogels
Chitosan
Complexation
Cisplatin
Glycols
Nanoparticles
Water
Platinum
Hydrogel
glycol-chitosan
Glucosamine
Polymer matrix
Light scattering
Pyrene
Methanol
Ion exchange
Polymers
Agglomeration
Hydrodynamics
Fluorescence

Keywords

  • Chitosan
  • Controlled drug release
  • Hydrogel
  • Microparticle
  • Nanoparticle
  • Platinum

ASJC Scopus subject areas

  • Polymers and Plastics
  • Materials Chemistry

Cite this

Cha, J., Won, B. L., Chong, R. P., Yong, W. C., Ahn, C. H., & Kwon, I. C. (2006). Preparation and characterization of cisplatin-incorporated chitosan hydrogels, microparticles, and nanoparticles. Macromolecular Research, 14(5), 573-578.

Preparation and characterization of cisplatin-incorporated chitosan hydrogels, microparticles, and nanoparticles. / Cha, Jueun; Won, Bum Lee; Chong, Rae Park; Yong, Woo Cho; Ahn, Cheol Hee; Kwon, Ick Chan.

In: Macromolecular Research, Vol. 14, No. 5, 01.10.2006, p. 573-578.

Research output: Contribution to journalArticle

Cha, J, Won, BL, Chong, RP, Yong, WC, Ahn, CH & Kwon, IC 2006, 'Preparation and characterization of cisplatin-incorporated chitosan hydrogels, microparticles, and nanoparticles', Macromolecular Research, vol. 14, no. 5, pp. 573-578.
Cha, Jueun ; Won, Bum Lee ; Chong, Rae Park ; Yong, Woo Cho ; Ahn, Cheol Hee ; Kwon, Ick Chan. / Preparation and characterization of cisplatin-incorporated chitosan hydrogels, microparticles, and nanoparticles. In: Macromolecular Research. 2006 ; Vol. 14, No. 5. pp. 573-578.
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