Preparation and characterization of nanoparticles containing trypsin based on hydrophobically modified chitosan

Chen Guang Liu, Kashappa Goud H Desai, Xi Guang Chen, Hyun Jin Park

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Trypsin was immobilized on linolenic acid modified chitosan using glutaraldehyde (GA) as cross-linker, which was confirmed by Fourier transform infrared (FTIR) spectra. The chitosan nanoparticles containing trypsin (TR) can be prepared after the sonication of immobilized trypsin. The GA concentration affected both the enzyme activity of the nanoparticle and particle size. Results indicated that the activity of trypsin immobilized onto linolenic acid modified chitosan nanoparticles increased with increasing concentration of GA up to 0.07% (v/v) and then decreased with increasing amount of GA. On the other hand, particle size increased (from 523 to 1372 nm) with the increasing concentration of GA (from 0.03 to 0.1% v/v). The enzyme catalytic characteristics of nanoparticle solution were also studied. The results showed that the kinetic constant value (Km) of TR immobilized on nanoparticle (71.9 mg/mL) was higher than that of pure TR (50.2 mg/mL). However, the thermal stability and optimum temperature of TR immobilized on nanoparticles improved, which make it more attractive in the application aspect.

Original languageEnglish
Pages (from-to)1728-1733
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume53
Issue number5
DOIs
Publication statusPublished - 2005 Mar 9

Fingerprint

Chitosan
nanoparticles
chitosan
Nanoparticles
Trypsin
trypsin
glutaraldehyde
Glutaral
alpha-Linolenic Acid
linolenic acid
Particle Size
particle size
Particle size
Sonication
Enzyme activity
Fourier Analysis
Enzymes
thermal stability
Fourier transforms
Thermodynamic stability

Keywords

  • Chitosan
  • Hydrophobically modified chitosan
  • Nanoparticles
  • Trypsin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Preparation and characterization of nanoparticles containing trypsin based on hydrophobically modified chitosan. / Liu, Chen Guang; Desai, Kashappa Goud H; Chen, Xi Guang; Park, Hyun Jin.

In: Journal of Agricultural and Food Chemistry, Vol. 53, No. 5, 09.03.2005, p. 1728-1733.

Research output: Contribution to journalArticle

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AU - Desai, Kashappa Goud H

AU - Chen, Xi Guang

AU - Park, Hyun Jin

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N2 - Trypsin was immobilized on linolenic acid modified chitosan using glutaraldehyde (GA) as cross-linker, which was confirmed by Fourier transform infrared (FTIR) spectra. The chitosan nanoparticles containing trypsin (TR) can be prepared after the sonication of immobilized trypsin. The GA concentration affected both the enzyme activity of the nanoparticle and particle size. Results indicated that the activity of trypsin immobilized onto linolenic acid modified chitosan nanoparticles increased with increasing concentration of GA up to 0.07% (v/v) and then decreased with increasing amount of GA. On the other hand, particle size increased (from 523 to 1372 nm) with the increasing concentration of GA (from 0.03 to 0.1% v/v). The enzyme catalytic characteristics of nanoparticle solution were also studied. The results showed that the kinetic constant value (Km) of TR immobilized on nanoparticle (71.9 mg/mL) was higher than that of pure TR (50.2 mg/mL). However, the thermal stability and optimum temperature of TR immobilized on nanoparticles improved, which make it more attractive in the application aspect.

AB - Trypsin was immobilized on linolenic acid modified chitosan using glutaraldehyde (GA) as cross-linker, which was confirmed by Fourier transform infrared (FTIR) spectra. The chitosan nanoparticles containing trypsin (TR) can be prepared after the sonication of immobilized trypsin. The GA concentration affected both the enzyme activity of the nanoparticle and particle size. Results indicated that the activity of trypsin immobilized onto linolenic acid modified chitosan nanoparticles increased with increasing concentration of GA up to 0.07% (v/v) and then decreased with increasing amount of GA. On the other hand, particle size increased (from 523 to 1372 nm) with the increasing concentration of GA (from 0.03 to 0.1% v/v). The enzyme catalytic characteristics of nanoparticle solution were also studied. The results showed that the kinetic constant value (Km) of TR immobilized on nanoparticle (71.9 mg/mL) was higher than that of pure TR (50.2 mg/mL). However, the thermal stability and optimum temperature of TR immobilized on nanoparticles improved, which make it more attractive in the application aspect.

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