Preparation and characterization of self-assembled nanoparticles of heparin-deoxycholic acid conjugates

Kyeongsoon Park, Kwang Meyung Kim, Ick Chan Kwon, Sang Kyoon Kim, Seulki Lee, Dong Yun Lee, Youngro Byun

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Various deoxycholic acid (DOCA) bearing heparin (HD) amphiphilic conjugates with different degrees of substitution (DS) with DOCA were synthesized using heparin as a hydrophilic segment and DOCA as a hydrophobic segment. Structural characteristics of these HD conjugates were investigated using 1H NMR, dynamic light scattering, zeta potential, transmission electron microscopy (TEM), and fluorescence spectroscopy. HD conjugates provided monodispersed self-aggregates in water, with mean diameters decreasing with increasing DOCA DS in the range of 120-200 nm. HD aggregates were covered with negatively charged heparin shells, exhibiting ξ potentials near -56 mV. The critical aggregation concentration (cac) of the HD (0.02-0.003 mg/mL) depended upon DOCA DS. TEM images demonstrated that the shape of the self-aggregates was spherical. Partition equilibrium constants, Kv, for pyrene in the self-aggregates in water indicated that increasing DS enhanced the hydrophobicity of the self-aggregate inner core. The mean aggregation number of DOCA per hydrophobic microdomain, estimated by the fluorescence quenching methods using cetylpyridinium chloride, indicated that five to nine of HD chains comprised a hydrophobic domain in the conjugates.

Original languageEnglish
Pages (from-to)11726-11731
Number of pages6
JournalLangmuir
Volume20
Issue number26
DOIs
Publication statusPublished - 2004 Dec 21
Externally publishedYes

Fingerprint

heparins
Deoxycholic Acid
Nanoparticles
Heparin
nanoparticles
preparation
acids
Acids
Substitution reactions
substitutes
Transmission Electron Microscopy
Bearings (structural)
Agglomeration
Cetylpyridinium
Transmission electron microscopy
fluorescence
transmission electron microscopy
Water
Fluorescence Spectrometry
Pyrene

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Preparation and characterization of self-assembled nanoparticles of heparin-deoxycholic acid conjugates. / Park, Kyeongsoon; Kim, Kwang Meyung; Kwon, Ick Chan; Kim, Sang Kyoon; Lee, Seulki; Lee, Dong Yun; Byun, Youngro.

In: Langmuir, Vol. 20, No. 26, 21.12.2004, p. 11726-11731.

Research output: Contribution to journalArticle

Park, Kyeongsoon ; Kim, Kwang Meyung ; Kwon, Ick Chan ; Kim, Sang Kyoon ; Lee, Seulki ; Lee, Dong Yun ; Byun, Youngro. / Preparation and characterization of self-assembled nanoparticles of heparin-deoxycholic acid conjugates. In: Langmuir. 2004 ; Vol. 20, No. 26. pp. 11726-11731.
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