Preparation and characterization of self-assembled nanoparticles based on glycol chitosan bearing adriamycin

Jae Hyung Park, Yong Woo Cho, Yoen Ju Son, Kwang Meyung Kim, Hesson Chung, Seo Young Jeong, Kuiwon Choi, Chong Rae Park, Rang Woon Park, In-San Kim, Ick Chan Kwon

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

An anthracycline drug, adriamycin, was chemically conjugated onto the backbone of glycol chitosan via an acid-labile cis-aconityl linkage. The physicochemical characteristics of the glycol chitosan-adriamycin (GC-ADR) conjugates were investigated by dynamic light scattering, atomic force microscopy, and fluorescence spectroscopy. The GC-ADR conjugates were capable of forming nano-sized self-aggregates in an aqueous medium, when the adriamycin content in the conjugate was in the range of 2.0-5.0 wt.%. The self-aggregates were spherical in shape, and had mean diameters of 238-304 nm, depending on the adriamycin content. The critical aggregation concentrations of the conjugates, estimated by the fluorescence quenching method, were as low as 1.0-2.5×10-2 mg/ml. The size of self-aggregates was not affected by the polymer concentration in the range from 50 to 2,000 μg/ml, and was maintained up to 8 days in phosphate-buffered saline (pH 7.4), indicating high colloidal stability. The release of adriamycin from self-aggregates was significantly dependent on the pH of the medium due to the cis-aconityl linkage; e.g., the amount of adriamycin released for 4 days was 7.3±0.3% at pH 7, whereas it was 29.3±1.9% at pH 4. The cell viability results demonstrated that free adriamycin shows more potent cytotoxicity than the conjugates, primarily attributed to the sustained release of adriamycin from self-aggregates. In conclusion, the self-aggregates, formed by GC-ADR conjugates, might be useful for the site-specific delivery of adriamycin in a sustained manner.

Original languageEnglish
Pages (from-to)763-770
Number of pages8
JournalColloid and Polymer Science
Volume284
Issue number7
DOIs
Publication statusPublished - 2006 Apr 1
Externally publishedYes

Fingerprint

Bearings (structural)
Glycols
Chitosan
Doxorubicin
glycols
Nanoparticles
nanoparticles
preparation
linkages
fluorescence
Fluorescence spectroscopy
Dynamic light scattering
Cytotoxicity
viability
glycol-chitosan
Quenching
Atomic force microscopy
delivery
phosphates
Phosphates

Keywords

  • Adriamycin
  • Cytotoxicity
  • Glycol chitosan
  • Self-aggregates
  • Sustained release

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Polymers and Plastics
  • Materials Chemistry
  • Colloid and Surface Chemistry

Cite this

Preparation and characterization of self-assembled nanoparticles based on glycol chitosan bearing adriamycin. / Park, Jae Hyung; Cho, Yong Woo; Son, Yoen Ju; Kim, Kwang Meyung; Chung, Hesson; Jeong, Seo Young; Choi, Kuiwon; Park, Chong Rae; Park, Rang Woon; Kim, In-San; Kwon, Ick Chan.

In: Colloid and Polymer Science, Vol. 284, No. 7, 01.04.2006, p. 763-770.

Research output: Contribution to journalArticle

Park, JH, Cho, YW, Son, YJ, Kim, KM, Chung, H, Jeong, SY, Choi, K, Park, CR, Park, RW, Kim, I-S & Kwon, IC 2006, 'Preparation and characterization of self-assembled nanoparticles based on glycol chitosan bearing adriamycin', Colloid and Polymer Science, vol. 284, no. 7, pp. 763-770. https://doi.org/10.1007/s00396-005-1438-7
Park, Jae Hyung ; Cho, Yong Woo ; Son, Yoen Ju ; Kim, Kwang Meyung ; Chung, Hesson ; Jeong, Seo Young ; Choi, Kuiwon ; Park, Chong Rae ; Park, Rang Woon ; Kim, In-San ; Kwon, Ick Chan. / Preparation and characterization of self-assembled nanoparticles based on glycol chitosan bearing adriamycin. In: Colloid and Polymer Science. 2006 ; Vol. 284, No. 7. pp. 763-770.
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AU - Choi, Kuiwon

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