Preparation, characterization, and protein loading properties of N-acyl chitosan nanoparticles

Youngjin Cho, Jun Tae Kim, Hyun Jin Park

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Various N-acyl chitosans with propionyl-, hexanoyl-, nonanoyl-, lauroyl-, pentadecanoyl-, and stearoyl-groups were synthesized and self-aggregated N-acyl chitosan nanoparticles (CSNPs) were prepared by sonication. By the modification with N-acyl groups, CSNPs increased their hydrophobic character and changed its structural features to be more suitable as a delivery carrier. The mean diameters of bovine serum albumin (BSA)-loaded N-acyl CSNPs ranged from 138 to 551 nm. Uniform particle size distribution of BSA-loaded N-acyl CSNPs was observed. The protein loading efficiency of N-acyl CSNPs was about 94-95% with lower BSA concentration (0.1 mg/mL) and not significantly different with acyl chain length. With higher BSA concentration (1.0 mg/mL), however, the highest protein loading efficiency was observed with lauroyl and pentadecanoyl CSNPs. The results suggest that lauroyl and pentadecanoyl CSs are interesting candidates for protein delivery system.

Original languageEnglish
Pages (from-to)1366-1371
Number of pages6
JournalJournal of Applied Polymer Science
Volume124
Issue number2
DOIs
Publication statusPublished - 2012 Apr 15

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Keywords

  • BSA
  • N-acyl chitosan
  • nanoparticle
  • protein loading efficiency

ASJC Scopus subject areas

  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Polymers and Plastics
  • Materials Chemistry

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