Preparation of a chemically anchored phospholipid monolayer on an acrylated polymer substrate

Hyun Kyun Kim, Kwang Meyung Kim, Youngro Byun

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

This paper describes a strategy for designing a chemically anchored phospholipid monolayer that could be used as coating materials for biomedical implants. To make a chemically anchored phospholipid monolayer on the polymer substrate, we prepared the mono-acrylated phospholipid (1-palmitoyl-2-[12- (acryloyloxy)-dodecanoyl]-sn-glycero-3-phosphocholine; acryloyl-PC) and the acrylated polymer (poly(octadecylacrylate-co-4-acryloyloxy butylacrylate)), which was synthesized by the acrylation of poly(octadecyl acrylate-co- hydroxybutyl acrylate, poly(OA-co-HA)) with acryloyl chloride. The chemically anchored phospholipid monolayer was prepared by using in situ photopolymerization of a pre-assembled phospholipid monolayer, produced by lipid vesicle fusion, onto the acrylated polymer coated silicon wafer. Optimal condition of vesicle fusion and irradiation time was determined from the degree of hydrophilicity rendered by the polymerized phospholipid surface. The physicochemical properties of polymerized phospholipid monolayer on the substrate were evaluated using water contact angle, field-emission scanning electron micrograph (FE-SEM), atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). These results confirmed that the polymerized phospholipid monolayer was chemically anchored on the acrylated polymer substrate. The chemically anchored phospholipid monolayer was stable in aqueous condition for 2 weeks, but the physically adsorbed phospholipid monolayer got removed within 1 day. Moreover, the polymerized phospholipid monolayer also suppressed albumin absorption and platelet adhesion, in vitro. This polymerized phospholipid monolayer provides a new biomimetic system for coating medical devises.

Original languageEnglish
Pages (from-to)3435-3444
Number of pages10
JournalBiomaterials
Volume26
Issue number17
DOIs
Publication statusPublished - 2005 Jun 1
Externally publishedYes

Fingerprint

Phospholipids
Monolayers
Polymers
Substrates
Fusion reactions
Photoelectron Spectroscopy
Coatings
Biomimetics
Phosphorylcholine
Photopolymerization
Atomic Force Microscopy
Hydrophilicity
Silicon
Platelets
Silicon wafers
Hydrophobic and Hydrophilic Interactions
Field emission
Lipids
Contact angle
Albumins

Keywords

  • Acrylated polymer substrate
  • Blood compatibility
  • In situ photopolymerization
  • Phospholipid monolayer

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Preparation of a chemically anchored phospholipid monolayer on an acrylated polymer substrate. / Kyun Kim, Hyun; Kim, Kwang Meyung; Byun, Youngro.

In: Biomaterials, Vol. 26, No. 17, 01.06.2005, p. 3435-3444.

Research output: Contribution to journalArticle

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