Preparation of biocatalytic nanofibres with high activity and stability via enzyme aggregate coating on polymer nanofibres

Byoung Chan Kim, Sujith Nair, Jungbae Kim, Ja Hun Kwak, Jay W. Grate, Seong H. Kim, Man Bock Gu

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

We have developed a unique approach for the fabrication of enzyme aggregate coatings on the surfaces of electrospun polymer nanofibres. This approach employs covalent attachment of seed enzymes onto nanofibres consisting of a mixture of polystyrene and poly(styrene-co-maleic anhydride), followed by a glutaraldehyde (GA) treatment that cross-links additional enzyme molecules and aggregates from the solution onto the covalently attached seed enzyme molecules. These cross-linked enzyme aggregates, covalently attached to the nanofibres via the linkers of seed enzyme molecules, are expected to improve the enzyme activity due to increased enzyme loading, and also the enzyme stability. To demonstrate the principle, we coated α-chymotrypsin (CT) on nanofibres electrospun from a mixture of polystyrene and poly(styrene-co-maleic anhydride). The initial activity of CT-aggregate-coated nanofibres was nine times higher than nanofibres with just a layer of covalently attached CT molecules. The enzyme stability of CT-aggregate-coated nanofibres was greatly improved with essentially no measurable loss of activity over a month of observation under rigorous shaking conditions. This new approach of enzyme coating on nanofibres, yielding high activity and stability, creates a useful new biocatalytic immobilized enzyme system with potential applications in bioconversion, bioremediation, and biosensors.

Original languageEnglish
JournalNanotechnology
Volume16
Issue number7
DOIs
Publication statusPublished - 2005 Jul 1

Fingerprint

Nanofibers
Enzyme Stability
enzymes
Polymers
Enzymes
coatings
Coatings
preparation
polymers
Chymotrypsin
polystyrene
Seeds
Seed
Polystyrenes
Molecules
seeds
Maleic anhydride
anhydrides
molecules
Styrene

ASJC Scopus subject areas

  • Engineering (miscellaneous)
  • Materials Science(all)
  • Physics and Astronomy (miscellaneous)

Cite this

Preparation of biocatalytic nanofibres with high activity and stability via enzyme aggregate coating on polymer nanofibres. / Kim, Byoung Chan; Nair, Sujith; Kim, Jungbae; Kwak, Ja Hun; Grate, Jay W.; Kim, Seong H.; Gu, Man Bock.

In: Nanotechnology, Vol. 16, No. 7, 01.07.2005.

Research output: Contribution to journalArticle

Kim, Byoung Chan ; Nair, Sujith ; Kim, Jungbae ; Kwak, Ja Hun ; Grate, Jay W. ; Kim, Seong H. ; Gu, Man Bock. / Preparation of biocatalytic nanofibres with high activity and stability via enzyme aggregate coating on polymer nanofibres. In: Nanotechnology. 2005 ; Vol. 16, No. 7.
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