Preparation of chitosan self-aggregates as a gene delivery system

K. Y. Lee, I. C. Kwon, Y. H. Kim, W. H. Jo, S. Y. Jeong

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375 Citations (Scopus)

Abstract

Hydrophobically modified chitosan containing 5.1 deoxycholic acid groups per 100 anhydroglucose units was synthesized by an EDC-mediated coupling reaction. Formation and characteristics of self-aggregates of hydrophobically modified chitosan were studied by fluorescence spectroscopy and dynamic light scattering method. The critical aggregation concentration (cac) of the self- aggregate was determined by measuring the fluorescence intensity of pyrene as a fluorescent probe. The cac value in PBS solution (pH 7.2) was 1.7 x 10-2 mg/ml. Mean diameter of self-aggregates in PBS solution (pH 7.2) was 162 ± 18 nm with an unimodal size distribution. Charge complex formation between self-aggregates and plasmid DNA was confirmed by electrophoresis on an agarose gel. Migration of DNA on an agarose gel was completely retarded above a charge ratio (+/-) of 4/1 at pH 7.2. The free DNA dissociated from the complexes was observed by electrophoresis above pH 8.0 at a fixed charge ratio of 4/1. An efficient transfection of COS-1 cells was achieved by self- aggregates/DNA complexes.

Original languageEnglish
Pages (from-to)213-220
Number of pages8
JournalJournal of Controlled Release
Volume51
Issue number2-3
DOIs
Publication statusPublished - 1998 Feb 12

Keywords

  • Cell transfection
  • Charge complex
  • Chitosan self-aggregate
  • Hydrophobic modification
  • Plasmid DNA

ASJC Scopus subject areas

  • Pharmaceutical Science

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  • Cite this

    Lee, K. Y., Kwon, I. C., Kim, Y. H., Jo, W. H., & Jeong, S. Y. (1998). Preparation of chitosan self-aggregates as a gene delivery system. Journal of Controlled Release, 51(2-3), 213-220. https://doi.org/10.1016/S0168-3659(97)00173-9