Preparation of cobalt nanoparticles from polymorphic bacterial templates

A novel platform for biocatalysis

Eunjin Jang, Hyun Woo Shim, Bum Han Ryu, Deu Rae An, Wan Ki Yoo, Kyeong Kyu Kim, Dong-Wan Kim, T. Doohun Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Nanoparticles have gathered significant research attention as materials for enzyme immobilization due to their advantageous properties such as low diffusion rates, ease of manipulation, and large surface areas. Here, polymorphic cobalt nanoparticles of varied sizes and shapes were prepared using Micrococcus lylae, Bacillus subtilis, Escherichia coli, Paracoccus sp., and Haloarcula vallismortis as bacterial templates. Furthermore, nine lipases/carboxylesterases were successfully immobilized on these cobalt nanoparticles. Especially, immobilized forms of Est-Y29, LmH, and Sm23 were characterized in more detail for potential industrial applications. Immobilization of enzymes onto cobalt oxide nanoparticles prepared from polymorphic bacterial templates may have potential for efficient hydrolysis on an industrial-scale, with several advantages such as high retention of enzymatic activity, increased stability, and strong reusability.

Original languageEnglish
Pages (from-to)747-753
Number of pages7
JournalInternational Journal of Biological Macromolecules
Volume81
DOIs
Publication statusPublished - 2015 Nov 1

Fingerprint

Biocatalysis
Cobalt
Nanoparticles
Immobilization
Haloarcula
Paracoccus
Carboxylic Ester Hydrolases
Enzyme immobilization
Micrococcus
Reusability
Bacilli
Enzymes
Bacillus subtilis
Lipase
Escherichia coli
Industrial applications
Hydrolysis
Research

Keywords

  • Biocatalysts
  • Cobalt oxide nanoparticles
  • Enzyme immobilization

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Cite this

Preparation of cobalt nanoparticles from polymorphic bacterial templates : A novel platform for biocatalysis. / Jang, Eunjin; Shim, Hyun Woo; Ryu, Bum Han; An, Deu Rae; Yoo, Wan Ki; Kim, Kyeong Kyu; Kim, Dong-Wan; Kim, T. Doohun.

In: International Journal of Biological Macromolecules, Vol. 81, 01.11.2015, p. 747-753.

Research output: Contribution to journalArticle

Jang, Eunjin ; Shim, Hyun Woo ; Ryu, Bum Han ; An, Deu Rae ; Yoo, Wan Ki ; Kim, Kyeong Kyu ; Kim, Dong-Wan ; Kim, T. Doohun. / Preparation of cobalt nanoparticles from polymorphic bacterial templates : A novel platform for biocatalysis. In: International Journal of Biological Macromolecules. 2015 ; Vol. 81. pp. 747-753.
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