Preparation of high purity docosahexaenoic acid from microalgae oil in a packed bed reactor via two-step lipase-catalysed esterification

Eun Ju Lee, Moon Won Lee, Da Som No, Hee Jin Kim, Se Wook Oh, Yangha Kim, In-Hwan Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Highly pure docosahexaenoic acid (DHA) was produced successfully in a packed bed reactor via the two-step lipase-catalysed esterification using the fatty acid from microalgae (from Crypthecodinium cohnii) and ethanol as substrates. Lipozyme RM IM from Rhizomucor miehei was employed as a biocatalyst. In the first step, a temperature of 40 °C, a molar ratio of 1:4 (fatty acid to ethanol), and a water content of 0.6 wt% (based on the total substrate weight) were selected as optimum conditions. A maximum DHA concentration of ca. 90 wt% was achieved in the fatty acid fraction with an 89 wt% yield after 15 min. In the second step, the fatty acids from the first step were used as the substrate and a recursive reaction was conducted using identical optimized conditions. Following this second step, a DHA concentration of 100 wt% was achieved in conjunction with an 83.2 wt% yield after 25 min.

Original languageEnglish
Pages (from-to)330-337
Number of pages8
JournalJournal of Functional Foods
Volume21
DOIs
Publication statusPublished - 2016 Mar 1

Keywords

  • Crypthecodinium cohnii
  • Docosahexaenoic acid (DHA)
  • Esterification
  • Lipase
  • Microalgae
  • Rhizomucor miehei

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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