Abstract
Highly pure docosahexaenoic acid (DHA) was produced successfully in a packed bed reactor via the two-step lipase-catalysed esterification using the fatty acid from microalgae (from Crypthecodinium cohnii) and ethanol as substrates. Lipozyme RM IM from Rhizomucor miehei was employed as a biocatalyst. In the first step, a temperature of 40 °C, a molar ratio of 1:4 (fatty acid to ethanol), and a water content of 0.6 wt% (based on the total substrate weight) were selected as optimum conditions. A maximum DHA concentration of ca. 90 wt% was achieved in the fatty acid fraction with an 89 wt% yield after 15 min. In the second step, the fatty acids from the first step were used as the substrate and a recursive reaction was conducted using identical optimized conditions. Following this second step, a DHA concentration of 100 wt% was achieved in conjunction with an 83.2 wt% yield after 25 min.
Original language | English |
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Pages (from-to) | 330-337 |
Number of pages | 8 |
Journal | Journal of Functional Foods |
Volume | 21 |
DOIs | |
Publication status | Published - 2016 Mar 1 |
Keywords
- Crypthecodinium cohnii
- Docosahexaenoic acid (DHA)
- Esterification
- Lipase
- Microalgae
- Rhizomucor miehei
ASJC Scopus subject areas
- Food Science
- Medicine (miscellaneous)
- Nutrition and Dietetics