Abstract
Highly pure docosahexaenoic acid (DHA) was produced successfully in a packed bed reactor via the two-step lipase-catalysed esterification using the fatty acid from microalgae (from Crypthecodinium cohnii) and ethanol as substrates. Lipozyme RM IM from Rhizomucor miehei was employed as a biocatalyst. In the first step, a temperature of 40 °C, a molar ratio of 1:4 (fatty acid to ethanol), and a water content of 0.6 wt% (based on the total substrate weight) were selected as optimum conditions. A maximum DHA concentration of ca. 90 wt% was achieved in the fatty acid fraction with an 89 wt% yield after 15 min. In the second step, the fatty acids from the first step were used as the substrate and a recursive reaction was conducted using identical optimized conditions. Following this second step, a DHA concentration of 100 wt% was achieved in conjunction with an 83.2 wt% yield after 25 min.
| Original language | English |
|---|---|
| Pages (from-to) | 330-337 |
| Number of pages | 8 |
| Journal | Journal of Functional Foods |
| Volume | 21 |
| DOIs | |
| Publication status | Published - 2016 Mar 1 |
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Keywords
- Crypthecodinium cohnii
- Docosahexaenoic acid (DHA)
- Esterification
- Lipase
- Microalgae
- Rhizomucor miehei
ASJC Scopus subject areas
- Food Science
- Medicine (miscellaneous)
- Nutrition and Dietetics
Cite this
Preparation of high purity docosahexaenoic acid from microalgae oil in a packed bed reactor via two-step lipase-catalysed esterification. / Lee, Eun Ju; Lee, Moon Won; No, Da Som; Kim, Hee Jin; Oh, Se Wook; Kim, Yangha; Kim, In-Hwan.
In: Journal of Functional Foods, Vol. 21, 01.03.2016, p. 330-337.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preparation of high purity docosahexaenoic acid from microalgae oil in a packed bed reactor via two-step lipase-catalysed esterification
AU - Lee, Eun Ju
AU - Lee, Moon Won
AU - No, Da Som
AU - Kim, Hee Jin
AU - Oh, Se Wook
AU - Kim, Yangha
AU - Kim, In-Hwan
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Highly pure docosahexaenoic acid (DHA) was produced successfully in a packed bed reactor via the two-step lipase-catalysed esterification using the fatty acid from microalgae (from Crypthecodinium cohnii) and ethanol as substrates. Lipozyme RM IM from Rhizomucor miehei was employed as a biocatalyst. In the first step, a temperature of 40 °C, a molar ratio of 1:4 (fatty acid to ethanol), and a water content of 0.6 wt% (based on the total substrate weight) were selected as optimum conditions. A maximum DHA concentration of ca. 90 wt% was achieved in the fatty acid fraction with an 89 wt% yield after 15 min. In the second step, the fatty acids from the first step were used as the substrate and a recursive reaction was conducted using identical optimized conditions. Following this second step, a DHA concentration of 100 wt% was achieved in conjunction with an 83.2 wt% yield after 25 min.
AB - Highly pure docosahexaenoic acid (DHA) was produced successfully in a packed bed reactor via the two-step lipase-catalysed esterification using the fatty acid from microalgae (from Crypthecodinium cohnii) and ethanol as substrates. Lipozyme RM IM from Rhizomucor miehei was employed as a biocatalyst. In the first step, a temperature of 40 °C, a molar ratio of 1:4 (fatty acid to ethanol), and a water content of 0.6 wt% (based on the total substrate weight) were selected as optimum conditions. A maximum DHA concentration of ca. 90 wt% was achieved in the fatty acid fraction with an 89 wt% yield after 15 min. In the second step, the fatty acids from the first step were used as the substrate and a recursive reaction was conducted using identical optimized conditions. Following this second step, a DHA concentration of 100 wt% was achieved in conjunction with an 83.2 wt% yield after 25 min.
KW - Crypthecodinium cohnii
KW - Docosahexaenoic acid (DHA)
KW - Esterification
KW - Lipase
KW - Microalgae
KW - Rhizomucor miehei
UR - http://www.scopus.com/inward/record.url?scp=84957874391&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84957874391&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2015.12.020
DO - 10.1016/j.jff.2015.12.020
M3 - Article
AN - SCOPUS:84957874391
VL - 21
SP - 330
EP - 337
JO - Journal of Functional Foods
JF - Journal of Functional Foods
SN - 1756-4646
ER -