Preparation of high purity docosahexaenoic acid from microalgae oil in a packed bed reactor via two-step lipase-catalysed esterification

Eun Ju Lee, Moon Won Lee, Da Som No, Hee Jin Kim, Se Wook Oh, Yangha Kim, In-Hwan Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Highly pure docosahexaenoic acid (DHA) was produced successfully in a packed bed reactor via the two-step lipase-catalysed esterification using the fatty acid from microalgae (from Crypthecodinium cohnii) and ethanol as substrates. Lipozyme RM IM from Rhizomucor miehei was employed as a biocatalyst. In the first step, a temperature of 40 °C, a molar ratio of 1:4 (fatty acid to ethanol), and a water content of 0.6 wt% (based on the total substrate weight) were selected as optimum conditions. A maximum DHA concentration of ca. 90 wt% was achieved in the fatty acid fraction with an 89 wt% yield after 15 min. In the second step, the fatty acids from the first step were used as the substrate and a recursive reaction was conducted using identical optimized conditions. Following this second step, a DHA concentration of 100 wt% was achieved in conjunction with an 83.2 wt% yield after 25 min.

Original languageEnglish
Pages (from-to)330-337
Number of pages8
JournalJournal of Functional Foods
Volume21
DOIs
Publication statusPublished - 2016 Mar 1

Fingerprint

Microalgae
Docosahexaenoic Acids
Esterification
esterification
Lipase
docosahexaenoic acid
microalgae
purity
Oils
Fatty Acids
fatty acids
oils
Ethanol
Crypthecodinium
Rhizomucor
ethanol
Rhizomucor miehei
water content
Weights and Measures
Temperature

Keywords

  • Crypthecodinium cohnii
  • Docosahexaenoic acid (DHA)
  • Esterification
  • Lipase
  • Microalgae
  • Rhizomucor miehei

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Preparation of high purity docosahexaenoic acid from microalgae oil in a packed bed reactor via two-step lipase-catalysed esterification. / Lee, Eun Ju; Lee, Moon Won; No, Da Som; Kim, Hee Jin; Oh, Se Wook; Kim, Yangha; Kim, In-Hwan.

In: Journal of Functional Foods, Vol. 21, 01.03.2016, p. 330-337.

Research output: Contribution to journalArticle

Lee, Eun Ju ; Lee, Moon Won ; No, Da Som ; Kim, Hee Jin ; Oh, Se Wook ; Kim, Yangha ; Kim, In-Hwan. / Preparation of high purity docosahexaenoic acid from microalgae oil in a packed bed reactor via two-step lipase-catalysed esterification. In: Journal of Functional Foods. 2016 ; Vol. 21. pp. 330-337.
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