Preparation of highly purified pinolenic acid from pine nut oil using a combination of enzymatic esterification and urea complexation

Da Som No, Ting Ting Zhao, Yangha Kim, Mi Ra Yoon, Jeom Sig Lee, In-Hwan Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Pinolenic acid (PLA) is a polyunsaturated fatty acid of plant origin. PLA has been successfully enriched according to a two-step process involving lipase-catalysed esterification and urea complexation. For the first step, the fatty acids present in pine nut oil were selectively esterified with lauryl alcohol using Candida rugosa lipase. Under the optimum conditions of 0.1% enzyme loading, 10% additional water, and 15 °C, PLA was enriched up to 43 mol% from an initial value of 13 mol% in the pine nut oil. For the second step, the PLA-enriched fraction from the first step was subjected to a urea complexation process. In this way, PLA enrichments with purities greater than 95 mol% were obtained at urea to fatty acid ratios greater than 3:1 (wt/wt), and 100% pure PLA was produced at a urea to fatty acid ratio of 5:1 with an 8.7 mol% yield.

Original languageEnglish
Pages (from-to)386-393
Number of pages8
JournalFood Chemistry
Volume170
DOIs
Publication statusPublished - 2015 Mar 1

Fingerprint

nut oils
Nuts
Esterification
esterification
Complexation
Urea
Oils
Pinus
urea
acids
Fatty Acids
Lipase
Dodecanol
fatty acid composition
Candida rugosa
Candida
Unsaturated Fatty Acids
purity
5,9,12-octadecatrienoic acid
polyunsaturated fatty acids

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Preparation of highly purified pinolenic acid from pine nut oil using a combination of enzymatic esterification and urea complexation. / No, Da Som; Zhao, Ting Ting; Kim, Yangha; Yoon, Mi Ra; Lee, Jeom Sig; Kim, In-Hwan.

In: Food Chemistry, Vol. 170, 01.03.2015, p. 386-393.

Research output: Contribution to journalArticle

No, Da Som ; Zhao, Ting Ting ; Kim, Yangha ; Yoon, Mi Ra ; Lee, Jeom Sig ; Kim, In-Hwan. / Preparation of highly purified pinolenic acid from pine nut oil using a combination of enzymatic esterification and urea complexation. In: Food Chemistry. 2015 ; Vol. 170. pp. 386-393.
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AU - Kim, In-Hwan

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