Preparation of highly purified pinolenic acid from pine nut oil using a combination of enzymatic esterification and urea complexation

Da Som No, Ting Ting Zhao, Yangha Kim, Mi Ra Yoon, Jeom Sig Lee, In-Hwan Kim

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Pinolenic acid (PLA) is a polyunsaturated fatty acid of plant origin. PLA has been successfully enriched according to a two-step process involving lipase-catalysed esterification and urea complexation. For the first step, the fatty acids present in pine nut oil were selectively esterified with lauryl alcohol using Candida rugosa lipase. Under the optimum conditions of 0.1% enzyme loading, 10% additional water, and 15 °C, PLA was enriched up to 43 mol% from an initial value of 13 mol% in the pine nut oil. For the second step, the PLA-enriched fraction from the first step was subjected to a urea complexation process. In this way, PLA enrichments with purities greater than 95 mol% were obtained at urea to fatty acid ratios greater than 3:1 (wt/wt), and 100% pure PLA was produced at a urea to fatty acid ratio of 5:1 with an 8.7 mol% yield.

Original languageEnglish
Pages (from-to)386-393
Number of pages8
JournalFood Chemistry
Publication statusPublished - 2015 Mar 1


ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

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