Preparation of highly purified stearidonic acid from echium oil via an enzymatic method combined with preparative high performance liquid chromatography

Ji Yeon Baik, Nam Ho Kim, Se Wook Oh, In-Hwan Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Stearidonic acid (SDA), an n-3 polyunsaturated fatty acid (PUFA), can be obtained from plant origin oils and it can be a good source of PUFA for vegetarians. SDA can be easily converted to longer PUFA such as docosahexaenoic acid and eicosapentaenoic acid. Highly purified stearidonic acid (SDA) was prepared successfully from echium oil via an enzymatic method combined with preparative high performance liquid chromatography. In the 1st step, SDA enrichment was accomplished using Candida rugosa lipase and 39.5% of SDA was obtained in the fatty acid fraction. Subsequently, the 1<sup>st</sup> reaction mixture was used for the 2<sup>nd</sup> enzymatic esterification without any separation process. The 2<sup>nd</sup> esterification was conducted for further SDA enrichment in a packed-bed reactor using Lipozyme RM IM from Rhizomucor miehei and the SDA content increased in a very short residence time. Ethanol was selected as an appropriate alcohol to react as an acyl receptor, and the other conditions for SDA enrichment were optimized at 20? of temperature, and 1:4 of molar ratio (i.e., fatty acid to ethanol). Under these conditions, 51.6% of SDA was obtained in the fatty acid fraction after a residence time of 15 min. Finally, highly purified SDA (purity, >99%) was obtained by prep-HPLC using the SDA-rich fraction obtained from the two-step lipase-catalyzed esterification.

Original languageEnglish
Pages (from-to)729-736
Number of pages8
JournalJournal of Oleo Science
Volume64
Issue number7
DOIs
Publication statusPublished - 2015 Jul 1

Fingerprint

Echium
High performance liquid chromatography
Oils
High Pressure Liquid Chromatography
Acids
Polyunsaturated fatty acids
Esterification
Fatty acids
Fatty Acids
Lipase
Unsaturated Fatty Acids
Lipases
stearidonic acid
Ethanol
Rhizomucor
Eicosapentaenoic Acid
Plant Oils
Docosahexaenoic Acids
Candida
Omega-3 Fatty Acids

Keywords

  • Echium oil
  • Enzymatic method
  • Packed-bed reactor
  • Stearidonic acid

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Preparation of highly purified stearidonic acid from echium oil via an enzymatic method combined with preparative high performance liquid chromatography. / Baik, Ji Yeon; Kim, Nam Ho; Oh, Se Wook; Kim, In-Hwan.

In: Journal of Oleo Science, Vol. 64, No. 7, 01.07.2015, p. 729-736.

Research output: Contribution to journalArticle

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