Preparation of highly stable oligo(ethylene glycol) derivatives- functionalized gold nanoparticles and their application in LSPR-based detection of PSA/ACT complex

Cuong Cao, Sang Jun Sim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A sandwich immunoassay for PSA/ACT complex detection based on gold nanoparticle aggregation using two probes was developed. The functionalized colloidal gold nanoparticles (AuNPs) showed highly stable not only in the presence of high ionic strength but also in a wide pH range. The functionalized AuNPs were tagged with PSA/ACT complex monoclonal antibody and goat PSA polyclonal antibody and served as the probes to induce aggregation of the colloidal particles. As a result, PSA/ACT complex was detected at concentrations as low as 1 ng/ml. This is the first time that a new aggregation sandwich-immunoassay technique using two gold probes has been used, and the results are generally applicable to other LSPR-based immunoassays.

Original languageEnglish
Pages (from-to)3754-3757
Number of pages4
JournalJournal of Nanoscience and Nanotechnology
Volume7
Issue number11
DOIs
Publication statusPublished - 2007 Nov 1
Externally publishedYes

Fingerprint

immunoassay
Ethylene Glycol
Ethylene glycol
Immunoassay
Gold
Nanoparticles
glycols
ethylene
Agglomeration
gold
Derivatives
antibodies
nanoparticles
preparation
probes
goats
Gold Colloid
Monoclonal antibodies
Ionic strength
Goats

Keywords

  • Localized surface plasmon resonance
  • Oligo(ethylene glycol)
  • Particle aggregation
  • Prostate cancer

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Materials Science (miscellaneous)
  • Engineering (miscellaneous)

Cite this

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abstract = "A sandwich immunoassay for PSA/ACT complex detection based on gold nanoparticle aggregation using two probes was developed. The functionalized colloidal gold nanoparticles (AuNPs) showed highly stable not only in the presence of high ionic strength but also in a wide pH range. The functionalized AuNPs were tagged with PSA/ACT complex monoclonal antibody and goat PSA polyclonal antibody and served as the probes to induce aggregation of the colloidal particles. As a result, PSA/ACT complex was detected at concentrations as low as 1 ng/ml. This is the first time that a new aggregation sandwich-immunoassay technique using two gold probes has been used, and the results are generally applicable to other LSPR-based immunoassays.",
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AU - Sim, Sang Jun

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AB - A sandwich immunoassay for PSA/ACT complex detection based on gold nanoparticle aggregation using two probes was developed. The functionalized colloidal gold nanoparticles (AuNPs) showed highly stable not only in the presence of high ionic strength but also in a wide pH range. The functionalized AuNPs were tagged with PSA/ACT complex monoclonal antibody and goat PSA polyclonal antibody and served as the probes to induce aggregation of the colloidal particles. As a result, PSA/ACT complex was detected at concentrations as low as 1 ng/ml. This is the first time that a new aggregation sandwich-immunoassay technique using two gold probes has been used, and the results are generally applicable to other LSPR-based immunoassays.

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KW - Prostate cancer

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