Preparation of lotus-leaf-like structured blood compatible poly(e{open}-caprolactone)-block-poly(l-lactic acid) copolymer film surfaces

Seung Il Kim, Jin Ik Lim, Bo Ram Lee, Cho Hay Mun, Youngmee Jung, Soo Hyun Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Lotus-leaf-like structured poly(e{open}-caprolactone)-block-poly(l-lactic acid) copolymer (PCL-b-PLLA) films cast using the solvent-nonsolvent casting method. PCL-b-PLLA was synthesized by the well-known copolymerization process, and was confirmed by 1H NMR analysis. The molecular weight of the synthesized PCL-b-PLLA was measured by gel permeation chromatography (GPC). The number-average (Mn), weight-average (Mw) molecular weights and polydispersity (Mw/Mn) were 3.9×104, 5.1×104, and 1.3, respectively. PCL-b-PLLA films were cast in vacuum conditions with various nonsolvent ratios. Tetrahydrofuran (THF) was used as solvent and ethanol was used as nonsolvent. Surface hydrophobicity was confirmed by the water contact angle. The water contact angle was increased from 90.9°±4.2° to 130.2°±3.6°. Water contact angle was found to be influenced by surface topography. The prepared film surfaces were characterized by scanning electron microscopy (SEM). Changes in crystalline property were characterized by X-ray diffraction (XRD). Platelet adhesion tests of the modified PCL-b-PLLA film surfaces were evaluated by platelet-rich plasma (PRP) and whole blood. Cell adhesive behavior on the modified film surfaces was evaluated by fibroblast cell culture. The prepared lotus-leaf-like structured film surfaces exhibited reduced platelet adhesion and an increased fibroblast cell proliferation ratio.

Original languageEnglish
Pages (from-to)28-35
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume114
DOIs
Publication statusPublished - 2014 Feb 1

Fingerprint

lactic acid
Lactic acid
leaves
blood
Lactic Acid
copolymers
Blood
Copolymers
preparation
Platelets
platelets
Contact angle
fibroblasts
Fibroblasts
Water
Blood Platelets
Molecular Weight
casts
molecular weight
Adhesion

Keywords

  • Blood compatibility
  • Cell proliferation
  • Lotus-leaf-like
  • PCL-b-PLLA
  • Surface modification

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Preparation of lotus-leaf-like structured blood compatible poly(e{open}-caprolactone)-block-poly(l-lactic acid) copolymer film surfaces. / Kim, Seung Il; Lim, Jin Ik; Lee, Bo Ram; Mun, Cho Hay; Jung, Youngmee; Kim, Soo Hyun.

In: Colloids and Surfaces B: Biointerfaces, Vol. 114, 01.02.2014, p. 28-35.

Research output: Contribution to journalArticle

Kim, Seung Il ; Lim, Jin Ik ; Lee, Bo Ram ; Mun, Cho Hay ; Jung, Youngmee ; Kim, Soo Hyun. / Preparation of lotus-leaf-like structured blood compatible poly(e{open}-caprolactone)-block-poly(l-lactic acid) copolymer film surfaces. In: Colloids and Surfaces B: Biointerfaces. 2014 ; Vol. 114. pp. 28-35.
@article{cd04c3a38b1840ed8e66c8f4c939bf3d,
title = "Preparation of lotus-leaf-like structured blood compatible poly(e{open}-caprolactone)-block-poly(l-lactic acid) copolymer film surfaces",
abstract = "Lotus-leaf-like structured poly(e{open}-caprolactone)-block-poly(l-lactic acid) copolymer (PCL-b-PLLA) films cast using the solvent-nonsolvent casting method. PCL-b-PLLA was synthesized by the well-known copolymerization process, and was confirmed by 1H NMR analysis. The molecular weight of the synthesized PCL-b-PLLA was measured by gel permeation chromatography (GPC). The number-average (Mn), weight-average (Mw) molecular weights and polydispersity (Mw/Mn) were 3.9×104, 5.1×104, and 1.3, respectively. PCL-b-PLLA films were cast in vacuum conditions with various nonsolvent ratios. Tetrahydrofuran (THF) was used as solvent and ethanol was used as nonsolvent. Surface hydrophobicity was confirmed by the water contact angle. The water contact angle was increased from 90.9°±4.2° to 130.2°±3.6°. Water contact angle was found to be influenced by surface topography. The prepared film surfaces were characterized by scanning electron microscopy (SEM). Changes in crystalline property were characterized by X-ray diffraction (XRD). Platelet adhesion tests of the modified PCL-b-PLLA film surfaces were evaluated by platelet-rich plasma (PRP) and whole blood. Cell adhesive behavior on the modified film surfaces was evaluated by fibroblast cell culture. The prepared lotus-leaf-like structured film surfaces exhibited reduced platelet adhesion and an increased fibroblast cell proliferation ratio.",
keywords = "Blood compatibility, Cell proliferation, Lotus-leaf-like, PCL-b-PLLA, Surface modification",
author = "Kim, {Seung Il} and Lim, {Jin Ik} and Lee, {Bo Ram} and Mun, {Cho Hay} and Youngmee Jung and Kim, {Soo Hyun}",
year = "2014",
month = "2",
day = "1",
doi = "10.1016/j.colsurfb.2013.09.038",
language = "English",
volume = "114",
pages = "28--35",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",

}

TY - JOUR

T1 - Preparation of lotus-leaf-like structured blood compatible poly(e{open}-caprolactone)-block-poly(l-lactic acid) copolymer film surfaces

AU - Kim, Seung Il

AU - Lim, Jin Ik

AU - Lee, Bo Ram

AU - Mun, Cho Hay

AU - Jung, Youngmee

AU - Kim, Soo Hyun

PY - 2014/2/1

Y1 - 2014/2/1

N2 - Lotus-leaf-like structured poly(e{open}-caprolactone)-block-poly(l-lactic acid) copolymer (PCL-b-PLLA) films cast using the solvent-nonsolvent casting method. PCL-b-PLLA was synthesized by the well-known copolymerization process, and was confirmed by 1H NMR analysis. The molecular weight of the synthesized PCL-b-PLLA was measured by gel permeation chromatography (GPC). The number-average (Mn), weight-average (Mw) molecular weights and polydispersity (Mw/Mn) were 3.9×104, 5.1×104, and 1.3, respectively. PCL-b-PLLA films were cast in vacuum conditions with various nonsolvent ratios. Tetrahydrofuran (THF) was used as solvent and ethanol was used as nonsolvent. Surface hydrophobicity was confirmed by the water contact angle. The water contact angle was increased from 90.9°±4.2° to 130.2°±3.6°. Water contact angle was found to be influenced by surface topography. The prepared film surfaces were characterized by scanning electron microscopy (SEM). Changes in crystalline property were characterized by X-ray diffraction (XRD). Platelet adhesion tests of the modified PCL-b-PLLA film surfaces were evaluated by platelet-rich plasma (PRP) and whole blood. Cell adhesive behavior on the modified film surfaces was evaluated by fibroblast cell culture. The prepared lotus-leaf-like structured film surfaces exhibited reduced platelet adhesion and an increased fibroblast cell proliferation ratio.

AB - Lotus-leaf-like structured poly(e{open}-caprolactone)-block-poly(l-lactic acid) copolymer (PCL-b-PLLA) films cast using the solvent-nonsolvent casting method. PCL-b-PLLA was synthesized by the well-known copolymerization process, and was confirmed by 1H NMR analysis. The molecular weight of the synthesized PCL-b-PLLA was measured by gel permeation chromatography (GPC). The number-average (Mn), weight-average (Mw) molecular weights and polydispersity (Mw/Mn) were 3.9×104, 5.1×104, and 1.3, respectively. PCL-b-PLLA films were cast in vacuum conditions with various nonsolvent ratios. Tetrahydrofuran (THF) was used as solvent and ethanol was used as nonsolvent. Surface hydrophobicity was confirmed by the water contact angle. The water contact angle was increased from 90.9°±4.2° to 130.2°±3.6°. Water contact angle was found to be influenced by surface topography. The prepared film surfaces were characterized by scanning electron microscopy (SEM). Changes in crystalline property were characterized by X-ray diffraction (XRD). Platelet adhesion tests of the modified PCL-b-PLLA film surfaces were evaluated by platelet-rich plasma (PRP) and whole blood. Cell adhesive behavior on the modified film surfaces was evaluated by fibroblast cell culture. The prepared lotus-leaf-like structured film surfaces exhibited reduced platelet adhesion and an increased fibroblast cell proliferation ratio.

KW - Blood compatibility

KW - Cell proliferation

KW - Lotus-leaf-like

KW - PCL-b-PLLA

KW - Surface modification

UR - http://www.scopus.com/inward/record.url?scp=84886302683&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84886302683&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2013.09.038

DO - 10.1016/j.colsurfb.2013.09.038

M3 - Article

VL - 114

SP - 28

EP - 35

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -