Preparation of pH-sensitive CaP nanoparticles coated with a phosphate-based block copolymer for efficient gene delivery

Sangmok Jang, Seonju Lee, Heejin Kim, Jiyeon Ham, Ji-Hun Seo, Yeongbong Mok, Minwoo Noh, Yan Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We have synthesized a phosphate-based block copolymer, PEG-b-PMOEP (poly(ethylene glycol)-b-poly(2-methacryloyloxyethyl phosphate)), with a narrow molecular weight distribution (PD = 1.06) by atomic transfer radical polymerization (ATRP), and have constructed calcium phosphate nanoparticles (CaPNs) coated with the block copolymer as an efficient and safe intracellular gene delivery carrier. The phosphate-mimic PMOEP block could be incorporated into the calcium phosphate (CaP) core to entrap pDNA, with the PEG block forming a shell to prevent uncontrolled growth of CaP precipitates and aggregates in physiological fluids. The CaPNs showed high colloidal stability at pH 7.4, but released entrapped pDNA at an endosomal pH of 5.0 through a pH-dependent protonation of phosphate moieties for efficient endosomal escape. The PEG-b-PMOEP/CaP/pDNA nanoparticles, which were formed simply by mixing, exhibited great potential as gene delivery carriers for future gene therapy applications due to their high transfection efficiency, low toxicity, and good stability under physiological conditions.

Original languageEnglish
Pages (from-to)4678-4685
Number of pages8
JournalPolymer
Volume53
Issue number21
DOIs
Publication statusPublished - 2012 Sep 28
Externally publishedYes

Fingerprint

Calcium phosphate
Block copolymers
Phosphates
Genes
Nanoparticles
Polyethylene glycols
Gene therapy
Protonation
Molecular weight distribution
Free radical polymerization
Toxicity
Precipitates
calcium phosphate
Fluids

Keywords

  • Block copolymer
  • Calcium phosphate
  • pH-sensitivity

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics

Cite this

Preparation of pH-sensitive CaP nanoparticles coated with a phosphate-based block copolymer for efficient gene delivery. / Jang, Sangmok; Lee, Seonju; Kim, Heejin; Ham, Jiyeon; Seo, Ji-Hun; Mok, Yeongbong; Noh, Minwoo; Lee, Yan.

In: Polymer, Vol. 53, No. 21, 28.09.2012, p. 4678-4685.

Research output: Contribution to journalArticle

Jang, Sangmok ; Lee, Seonju ; Kim, Heejin ; Ham, Jiyeon ; Seo, Ji-Hun ; Mok, Yeongbong ; Noh, Minwoo ; Lee, Yan. / Preparation of pH-sensitive CaP nanoparticles coated with a phosphate-based block copolymer for efficient gene delivery. In: Polymer. 2012 ; Vol. 53, No. 21. pp. 4678-4685.
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