Preparation of phytosteryl ester and simultaneous enrichment of stearidonic acid via lipase-catalyzed esterification

Nakyung Choi, Hyo Jung Cho, Heejin Kim, Yangha Kim, Hak Ryul Kim, In-Hwan Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The simultaneous synthesis of a phytosteryl ester and enrichment of stearidonic acid (SDA) were performed via a one-step lipase-catalyzed esterification of fatty acids from Ahiflower™ seed oil with phytosterol. A commercial lipase (Lipase OF) from Candida rugosa was employed as a biocatalyst. Three solvents were screened and cyclohexane was selected as a suitable reaction medium. The effects of enzyme loading, temperature, and solvent amount were investigated. The conversion as well as the SDA content were significantly improved by adding molecular sieves after 1. h of reaction. The optimum conditions were the enzyme loading of 10% (based on the total substrate weight), the temperature of 30. °C, and the solvent amount of 4. mL (based on a substrate weight of 2. g), respectively. The maximum conversion and SDA content in the residual fatty acid were 81. mol% and 58 mol%, respectively, under the optimum conditions. The SDA content increased a 3.4-fold from 17 mol% in Ahiflower™ seed oil to 58. mol%.

Original languageEnglish
JournalProcess Biochemistry
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Esterification
Lipases
Lipase
Esters
Oilseeds
Acids
Fatty acids
Seeds
Oils
Enzymes
Fatty Acids
Weights and Measures
Phytosterols
Biocatalysts
Temperature
Candida
Molecular sieves
Substrates
Cyclohexane
stearidonic acid

Keywords

  • Ahiflower™ seed oil
  • Candida rugosa lipase
  • Esterification
  • Phytosterol
  • Phytosteryl ester
  • Stearidonic acid

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Preparation of phytosteryl ester and simultaneous enrichment of stearidonic acid via lipase-catalyzed esterification. / Choi, Nakyung; Cho, Hyo Jung; Kim, Heejin; Kim, Yangha; Kim, Hak Ryul; Kim, In-Hwan.

In: Process Biochemistry, 2017.

Research output: Contribution to journalArticle

@article{493b16092bb14b2e8fc432ce7662e2e9,
title = "Preparation of phytosteryl ester and simultaneous enrichment of stearidonic acid via lipase-catalyzed esterification",
abstract = "The simultaneous synthesis of a phytosteryl ester and enrichment of stearidonic acid (SDA) were performed via a one-step lipase-catalyzed esterification of fatty acids from Ahiflower™ seed oil with phytosterol. A commercial lipase (Lipase OF) from Candida rugosa was employed as a biocatalyst. Three solvents were screened and cyclohexane was selected as a suitable reaction medium. The effects of enzyme loading, temperature, and solvent amount were investigated. The conversion as well as the SDA content were significantly improved by adding molecular sieves after 1. h of reaction. The optimum conditions were the enzyme loading of 10{\%} (based on the total substrate weight), the temperature of 30. °C, and the solvent amount of 4. mL (based on a substrate weight of 2. g), respectively. The maximum conversion and SDA content in the residual fatty acid were 81. mol{\%} and 58 mol{\%}, respectively, under the optimum conditions. The SDA content increased a 3.4-fold from 17 mol{\%} in Ahiflower™ seed oil to 58. mol{\%}.",
keywords = "Ahiflower™ seed oil, Candida rugosa lipase, Esterification, Phytosterol, Phytosteryl ester, Stearidonic acid",
author = "Nakyung Choi and Cho, {Hyo Jung} and Heejin Kim and Yangha Kim and Kim, {Hak Ryul} and In-Hwan Kim",
year = "2017",
doi = "10.1016/j.procbio.2017.06.013",
language = "English",
journal = "Process Biochemistry",
issn = "1359-5113",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Preparation of phytosteryl ester and simultaneous enrichment of stearidonic acid via lipase-catalyzed esterification

AU - Choi, Nakyung

AU - Cho, Hyo Jung

AU - Kim, Heejin

AU - Kim, Yangha

AU - Kim, Hak Ryul

AU - Kim, In-Hwan

PY - 2017

Y1 - 2017

N2 - The simultaneous synthesis of a phytosteryl ester and enrichment of stearidonic acid (SDA) were performed via a one-step lipase-catalyzed esterification of fatty acids from Ahiflower™ seed oil with phytosterol. A commercial lipase (Lipase OF) from Candida rugosa was employed as a biocatalyst. Three solvents were screened and cyclohexane was selected as a suitable reaction medium. The effects of enzyme loading, temperature, and solvent amount were investigated. The conversion as well as the SDA content were significantly improved by adding molecular sieves after 1. h of reaction. The optimum conditions were the enzyme loading of 10% (based on the total substrate weight), the temperature of 30. °C, and the solvent amount of 4. mL (based on a substrate weight of 2. g), respectively. The maximum conversion and SDA content in the residual fatty acid were 81. mol% and 58 mol%, respectively, under the optimum conditions. The SDA content increased a 3.4-fold from 17 mol% in Ahiflower™ seed oil to 58. mol%.

AB - The simultaneous synthesis of a phytosteryl ester and enrichment of stearidonic acid (SDA) were performed via a one-step lipase-catalyzed esterification of fatty acids from Ahiflower™ seed oil with phytosterol. A commercial lipase (Lipase OF) from Candida rugosa was employed as a biocatalyst. Three solvents were screened and cyclohexane was selected as a suitable reaction medium. The effects of enzyme loading, temperature, and solvent amount were investigated. The conversion as well as the SDA content were significantly improved by adding molecular sieves after 1. h of reaction. The optimum conditions were the enzyme loading of 10% (based on the total substrate weight), the temperature of 30. °C, and the solvent amount of 4. mL (based on a substrate weight of 2. g), respectively. The maximum conversion and SDA content in the residual fatty acid were 81. mol% and 58 mol%, respectively, under the optimum conditions. The SDA content increased a 3.4-fold from 17 mol% in Ahiflower™ seed oil to 58. mol%.

KW - Ahiflower™ seed oil

KW - Candida rugosa lipase

KW - Esterification

KW - Phytosterol

KW - Phytosteryl ester

KW - Stearidonic acid

UR - http://www.scopus.com/inward/record.url?scp=85024111346&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85024111346&partnerID=8YFLogxK

U2 - 10.1016/j.procbio.2017.06.013

DO - 10.1016/j.procbio.2017.06.013

M3 - Article

AN - SCOPUS:85024111346

JO - Process Biochemistry

JF - Process Biochemistry

SN - 1359-5113

ER -