Preparation of phytosteryl ester and simultaneous enrichment of stearidonic acid via lipase-catalyzed esterification

Nakyung Choi, Hyo Jung Cho, Heejin Kim, Yangha Kim, Hak Ryul Kim, In-Hwan Kim

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3 Citations (Scopus)


The simultaneous synthesis of a phytosteryl ester and enrichment of stearidonic acid (SDA) were performed via a one-step lipase-catalyzed esterification of fatty acids from Ahiflower™ seed oil with phytosterol. A commercial lipase (Lipase OF) from Candida rugosa was employed as a biocatalyst. Three solvents were screened and cyclohexane was selected as a suitable reaction medium. The effects of enzyme loading, temperature, and solvent amount were investigated. The conversion as well as the SDA content were significantly improved by adding molecular sieves after 1. h of reaction. The optimum conditions were the enzyme loading of 10% (based on the total substrate weight), the temperature of 30. °C, and the solvent amount of 4. mL (based on a substrate weight of 2. g), respectively. The maximum conversion and SDA content in the residual fatty acid were 81. mol% and 58 mol%, respectively, under the optimum conditions. The SDA content increased a 3.4-fold from 17 mol% in Ahiflower™ seed oil to 58. mol%.

Original languageEnglish
JournalProcess Biochemistry
Publication statusAccepted/In press - 2017



  • Ahiflower™ seed oil
  • Candida rugosa lipase
  • Esterification
  • Phytosterol
  • Phytosteryl ester
  • Stearidonic acid

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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