Presence of secretogranin II and high-capacity, low-affinity Ca 2+ storage role in nucleoplasmic Ca2+ store vesicles

Hyun Yoo Seung, Yoon Chu Sei, Ki Deok Kim, Hoon Huh Yang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Chromogranins and secretogranins have traditionally been known as marker proteins of secretory granules that contain the highest concentrations of cellular calcium, reaching ∼40 mM. In addition, chromogranin B was also shown to exist in the nucleus, localizing in the putative inositol 1,4,5-trisphosphate (IP3)-sensitive nucleoplasmic Ca2+ store vesicles. Chromogranins A (CGA) and B (CGB) are high-capacity, low-affinity Ca2+ binding proteins, binding 30-90 mol of Ca 2+/mol with dissociation constants (Kd) of 1.5-4 mM. Yet the Ca 2+-binding property of secretogranins has not been studied. Here, we show the localization of secretogranin II (SgII) in the nucleus, more specifically, in the IP3-sensitive nucleoplasmic Ca2+ store vesicles along with CGB and the IP3 receptors. We have also determined the Ca2+-binding property of SgII and found that SgII binds 61 mol of Ca2+/mol (910 nmol Ca2+/mg) with a Kd of 3.0 mM at the intragranular pH 5.5 and 30 mol of Ca2+/mol (440 nmol Ca2+/mg) with a Kd of 2.2 mM at a near-physiological pH 7.5. Chromogranin B also bound 50 mol of Ca2+/mol (670 nmol Ca 2+/mg) with a Kd of 3.1 mM at pH 7.5. Given the high-capacity, low-affinity Ca2+-binding property of SgII and its presence in the IP3-sensitive nucleoplasmic Ca2+ store vesicles, these results suggest that SgII may function in the storage and control of Ca 2+ in the nucleus through its interaction with CGB in the nucleoplasmic vesicles.

Original languageEnglish
Pages (from-to)14663-14671
Number of pages9
JournalBiochemistry
Volume46
Issue number50
DOIs
Publication statusPublished - 2007 Dec 18
Externally publishedYes

Fingerprint

Secretogranin II
Chromogranin B
Chromogranins
Inositol 1,4,5-Trisphosphate Receptors
Chromogranin A
Inositol 1,4,5-Trisphosphate
Secretory Vesicles
Protein Binding
Carrier Proteins
Calcium
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Presence of secretogranin II and high-capacity, low-affinity Ca 2+ storage role in nucleoplasmic Ca2+ store vesicles. / Seung, Hyun Yoo; Sei, Yoon Chu; Kim, Ki Deok; Yang, Hoon Huh.

In: Biochemistry, Vol. 46, No. 50, 18.12.2007, p. 14663-14671.

Research output: Contribution to journalArticle

Seung, Hyun Yoo ; Sei, Yoon Chu ; Kim, Ki Deok ; Yang, Hoon Huh. / Presence of secretogranin II and high-capacity, low-affinity Ca 2+ storage role in nucleoplasmic Ca2+ store vesicles. In: Biochemistry. 2007 ; Vol. 46, No. 50. pp. 14663-14671.
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