Preso regulation of dendritic outgrowth through PI(4,5)P2-dependent PDZ interaction with βPix

Jiwon Mo, Dongmin Lee, Soontaek Hong, Seungrie Han, Hyojin Yeo, Woong Sun, Sukwoo Choi, Hyun Kim, Hyun Woo Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In neuronal development, dendritic outgrowth and arborization are important for the establishment of neural circuit formation. A previous study reported that PSD-95-interacting regulator of spine morphogenesis (Preso) formed a complex with PAK-interacting exchange factor-beta (βPix) via PSD-95/Dlg/ZO-1 (PDZ) interaction. Here, we report that Preso and its binding protein, βPix, are localized in dendritic growth cones. Knockdown and dominant-negative inhibition of Preso in cultured neurons markedly reduced the dendritic outgrowth but not branching, and led to a decrease in the intensity of βPix and F-actin in neuronal dendritic tips. Moreover, phosphatidylinositol 4,5-bisphosphate (PIP2) induced a conformational change in Preso toward the open PDZ domain and enhanced the interaction with βPix. In addition, the Preso band4.1 protein, ezrin, radixin and moesin (FERM) domain mutant is unable to interact with PIP2 and it did not rescue the Preso-knockdown effect. These results indicate that PIP2 is a key signalling molecule that regulates dendritic outgrowth through activation of small GTPase signalling via interaction between Preso and βPix.

Original languageEnglish
Pages (from-to)1960-1970
Number of pages11
JournalEuropean Journal of Neuroscience
Volume36
Issue number1
DOIs
Publication statusPublished - 2012 Jul 1

Keywords

  • Dendritic growth cones
  • F-actin
  • FERM domain
  • Hippocampal neuron

ASJC Scopus subject areas

  • Neuroscience(all)

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