Pretreatment of lipase with soybean oil before immobilization to prevent loss of activity

Dong Hwan Lee, Jung Mo Kim, Seong Woo Kang, Ji Won Lee, Seung Wook Kim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. This pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. The activity of the pretreated lipase immobilized covalently on a silica gel was 530 U/g-matrix, which is 16 times higher than that of the immobilized non-pretreated lipase. In addition, the immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.

Original languageEnglish
Pages (from-to)1965-1969
Number of pages5
JournalBiotechnology Letters
Volume28
Issue number23
DOIs
Publication statusPublished - 2006 Dec 1

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Keywords

  • Immobilization
  • Lipase
  • Pretreatment
  • Reuse
  • Soybean oil

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

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