Prevalence and molecular characterizations of Toxoplasma gondii and Babesia microti from small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea

Sung Hee Hong, Sang Eun Lee, Young Il Jeong, Heung Chul Kim, Sung Tae Chong, Terry A. Klein, Jin Won Song, Se Hun Gu, Shin Hyeong Cho, Won Ja Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A survey was conducted to determine the distribution and prevalence of Toxoplasma gondii and Babesia microti infections in small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea (ROK). The serological prevalence of T. gondii (ELISA) and B. microti (IFAT) was 2.3% (15/667) and 2.1% (14/667), respectively. DNA extracts from small mammal heart tissues were screened by PCR for T. gondii and B. microti targeting regions of the GRA5 gene and the 18S rRNA and β-tubulin genes, respectively. Only 0.17% (1/578) of Apodemus agrarius was positive of T. gondii by PCR, while 0.52% (3/578) was positive of B. microti. All other small mammal species [Micromys minutus (16), Mus musculus (3), Myodes regulus (22), Microtus fortis (6), and Crocidura lasiura (42)] were negative for both T. gondii and B. microti. Based on sequence polymorphism and phylogenetic analysis, T. gondii closely aligned with Type I, a highly virulent strain, while B. microti positive samples closely aligned with US-type B. microti and others observed in the ROK, Russia, and Japan. These results indicate that A. agrarius is a reservoir for both T. gondii and B. microti in the ROK.

Original languageEnglish
Pages (from-to)512-517
Number of pages6
JournalVeterinary Parasitology
Volume205
Issue number3-4
DOIs
Publication statusPublished - 2014

Fingerprint

Babesia microti
Republic of Korea
Toxoplasma
South Korea
Toxoplasma gondii
small mammals
Mammals
Apodemus agrarius
Arvicolinae
Crocidura
Murinae
Polymerase Chain Reaction
Microtus
Russia
Mus musculus
Tubulin
tubulin
rRNA Genes
Japan
genes

Keywords

  • Apodemus agrarius
  • Babesia microti
  • Republic of Korea
  • Toxoplsama gondii

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

Cite this

Prevalence and molecular characterizations of Toxoplasma gondii and Babesia microti from small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea. / Hong, Sung Hee; Lee, Sang Eun; Jeong, Young Il; Kim, Heung Chul; Chong, Sung Tae; Klein, Terry A.; Song, Jin Won; Gu, Se Hun; Cho, Shin Hyeong; Lee, Won Ja.

In: Veterinary Parasitology, Vol. 205, No. 3-4, 2014, p. 512-517.

Research output: Contribution to journalArticle

Hong, Sung Hee ; Lee, Sang Eun ; Jeong, Young Il ; Kim, Heung Chul ; Chong, Sung Tae ; Klein, Terry A. ; Song, Jin Won ; Gu, Se Hun ; Cho, Shin Hyeong ; Lee, Won Ja. / Prevalence and molecular characterizations of Toxoplasma gondii and Babesia microti from small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea. In: Veterinary Parasitology. 2014 ; Vol. 205, No. 3-4. pp. 512-517.
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abstract = "A survey was conducted to determine the distribution and prevalence of Toxoplasma gondii and Babesia microti infections in small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea (ROK). The serological prevalence of T. gondii (ELISA) and B. microti (IFAT) was 2.3{\%} (15/667) and 2.1{\%} (14/667), respectively. DNA extracts from small mammal heart tissues were screened by PCR for T. gondii and B. microti targeting regions of the GRA5 gene and the 18S rRNA and β-tubulin genes, respectively. Only 0.17{\%} (1/578) of Apodemus agrarius was positive of T. gondii by PCR, while 0.52{\%} (3/578) was positive of B. microti. All other small mammal species [Micromys minutus (16), Mus musculus (3), Myodes regulus (22), Microtus fortis (6), and Crocidura lasiura (42)] were negative for both T. gondii and B. microti. Based on sequence polymorphism and phylogenetic analysis, T. gondii closely aligned with Type I, a highly virulent strain, while B. microti positive samples closely aligned with US-type B. microti and others observed in the ROK, Russia, and Japan. These results indicate that A. agrarius is a reservoir for both T. gondii and B. microti in the ROK.",
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AU - Chong, Sung Tae

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