Prevalence and molecular epidemiology of vancomycin-resistant enterococci (VRE) strains isolated from animals and humans in Korea

Joon-Young Song, In Sook Hwang, Joong Sik Eom, Hee-Jin Cheong, Won Ki Bae, Yong Ho Park, Woo Joo Kim

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Abstract

Background: To assess the possibility of VRE transmission from animals to humans, we studied the prevalence of vancomycin-resistant enterococci (VRE) in farm animals, raw chicken meat, and healthy people. We then determined the molecular relatedness of VRE isolates between animals and humans in Korea. Methods: We aimed to isolate VRE from 150 enterococci specimens of farm animals, 15 raw chicken meat samples, and stools from 200 healthy people. Species differentiation was done with conventional biochemical tests. Vancomycin resistance genotyping was done by polymerase chain reaction (PCR). Using the agar dilution method, antimicrobial susceptibility was tested for 8 antimicrobials and pulsed-field gel electrophoresis (PFGE) was done to evaluate the molecular relatedness of VRE isolates. Results: The prevalence of VRE was 14.7% (22/150) in farm animal specimens, 1% (2/200) in healthy people, and 60% (9/15) in raw chicken meat. Of 22 animal VRE isolates, 1 vanA E. faecium, 15 vanC1 E. gallinarum, and 6 vanC2 E. casseliflavus were identified. All of the 9 VRE from raw chicken meat and all of the 20 clinical VRE strains were vanA E. faecium. However, in healthy people, only 2 vanC2 E. casselflavus were isolated. These showed low-level resistance to vancomycin and susceptibility to teicoplanin. However, 9 VRE strains from raw chicken meat had high-level resistance to vancomycin (MIC50,90: > 128 μg/mL), teicoplanin (MIC50,90: > 128 μg/mL), ampicillin (MIC50:,90: > 128 μg/mL), erythromycin (MIC50,90: > 128 μg/mL), and tetracycline (MIC50/90: 128/>128 μg/mL). Conclusion: This study demonstrated little evidence of VRE colonization in healthy people despite high recovery of VRE among raw chicken meat. It is suggested that there is little evidence of VRE transmission from animals to healthy people. However, we assumed that there exists the possibility of VRE contamination during the processing of chicken meat.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalKorean Journal of Internal Medicine
Volume20
Issue number1
Publication statusPublished - 2005 Mar 1

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Molecular Epidemiology
Korea
Meat
Chickens
Vancomycin Resistance
Domestic Animals
Teicoplanin
Vancomycin-Resistant Enterococci
Pulsed Field Gel Electrophoresis
Enterococcus
Erythromycin
Ampicillin
Tetracycline
Agar

Keywords

  • Domestic animals
  • Healthy person
  • PCR
  • PFGE (electrophoresis, gel, pulsed-field)
  • Vancomycin-resistant enterococcus

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Prevalence and molecular epidemiology of vancomycin-resistant enterococci (VRE) strains isolated from animals and humans in Korea. / Song, Joon-Young; Hwang, In Sook; Eom, Joong Sik; Cheong, Hee-Jin; Bae, Won Ki; Park, Yong Ho; Kim, Woo Joo.

In: Korean Journal of Internal Medicine, Vol. 20, No. 1, 01.03.2005, p. 55-62.

Research output: Contribution to journalArticle

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abstract = "Background: To assess the possibility of VRE transmission from animals to humans, we studied the prevalence of vancomycin-resistant enterococci (VRE) in farm animals, raw chicken meat, and healthy people. We then determined the molecular relatedness of VRE isolates between animals and humans in Korea. Methods: We aimed to isolate VRE from 150 enterococci specimens of farm animals, 15 raw chicken meat samples, and stools from 200 healthy people. Species differentiation was done with conventional biochemical tests. Vancomycin resistance genotyping was done by polymerase chain reaction (PCR). Using the agar dilution method, antimicrobial susceptibility was tested for 8 antimicrobials and pulsed-field gel electrophoresis (PFGE) was done to evaluate the molecular relatedness of VRE isolates. Results: The prevalence of VRE was 14.7{\%} (22/150) in farm animal specimens, 1{\%} (2/200) in healthy people, and 60{\%} (9/15) in raw chicken meat. Of 22 animal VRE isolates, 1 vanA E. faecium, 15 vanC1 E. gallinarum, and 6 vanC2 E. casseliflavus were identified. All of the 9 VRE from raw chicken meat and all of the 20 clinical VRE strains were vanA E. faecium. However, in healthy people, only 2 vanC2 E. casselflavus were isolated. These showed low-level resistance to vancomycin and susceptibility to teicoplanin. However, 9 VRE strains from raw chicken meat had high-level resistance to vancomycin (MIC50,90: > 128 μg/mL), teicoplanin (MIC50,90: > 128 μg/mL), ampicillin (MIC50:,90: > 128 μg/mL), erythromycin (MIC50,90: > 128 μg/mL), and tetracycline (MIC50/90: 128/>128 μg/mL). Conclusion: This study demonstrated little evidence of VRE colonization in healthy people despite high recovery of VRE among raw chicken meat. It is suggested that there is little evidence of VRE transmission from animals to healthy people. However, we assumed that there exists the possibility of VRE contamination during the processing of chicken meat.",
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T1 - Prevalence and molecular epidemiology of vancomycin-resistant enterococci (VRE) strains isolated from animals and humans in Korea

AU - Song, Joon-Young

AU - Hwang, In Sook

AU - Eom, Joong Sik

AU - Cheong, Hee-Jin

AU - Bae, Won Ki

AU - Park, Yong Ho

AU - Kim, Woo Joo

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N2 - Background: To assess the possibility of VRE transmission from animals to humans, we studied the prevalence of vancomycin-resistant enterococci (VRE) in farm animals, raw chicken meat, and healthy people. We then determined the molecular relatedness of VRE isolates between animals and humans in Korea. Methods: We aimed to isolate VRE from 150 enterococci specimens of farm animals, 15 raw chicken meat samples, and stools from 200 healthy people. Species differentiation was done with conventional biochemical tests. Vancomycin resistance genotyping was done by polymerase chain reaction (PCR). Using the agar dilution method, antimicrobial susceptibility was tested for 8 antimicrobials and pulsed-field gel electrophoresis (PFGE) was done to evaluate the molecular relatedness of VRE isolates. Results: The prevalence of VRE was 14.7% (22/150) in farm animal specimens, 1% (2/200) in healthy people, and 60% (9/15) in raw chicken meat. Of 22 animal VRE isolates, 1 vanA E. faecium, 15 vanC1 E. gallinarum, and 6 vanC2 E. casseliflavus were identified. All of the 9 VRE from raw chicken meat and all of the 20 clinical VRE strains were vanA E. faecium. However, in healthy people, only 2 vanC2 E. casselflavus were isolated. These showed low-level resistance to vancomycin and susceptibility to teicoplanin. However, 9 VRE strains from raw chicken meat had high-level resistance to vancomycin (MIC50,90: > 128 μg/mL), teicoplanin (MIC50,90: > 128 μg/mL), ampicillin (MIC50:,90: > 128 μg/mL), erythromycin (MIC50,90: > 128 μg/mL), and tetracycline (MIC50/90: 128/>128 μg/mL). Conclusion: This study demonstrated little evidence of VRE colonization in healthy people despite high recovery of VRE among raw chicken meat. It is suggested that there is little evidence of VRE transmission from animals to healthy people. However, we assumed that there exists the possibility of VRE contamination during the processing of chicken meat.

AB - Background: To assess the possibility of VRE transmission from animals to humans, we studied the prevalence of vancomycin-resistant enterococci (VRE) in farm animals, raw chicken meat, and healthy people. We then determined the molecular relatedness of VRE isolates between animals and humans in Korea. Methods: We aimed to isolate VRE from 150 enterococci specimens of farm animals, 15 raw chicken meat samples, and stools from 200 healthy people. Species differentiation was done with conventional biochemical tests. Vancomycin resistance genotyping was done by polymerase chain reaction (PCR). Using the agar dilution method, antimicrobial susceptibility was tested for 8 antimicrobials and pulsed-field gel electrophoresis (PFGE) was done to evaluate the molecular relatedness of VRE isolates. Results: The prevalence of VRE was 14.7% (22/150) in farm animal specimens, 1% (2/200) in healthy people, and 60% (9/15) in raw chicken meat. Of 22 animal VRE isolates, 1 vanA E. faecium, 15 vanC1 E. gallinarum, and 6 vanC2 E. casseliflavus were identified. All of the 9 VRE from raw chicken meat and all of the 20 clinical VRE strains were vanA E. faecium. However, in healthy people, only 2 vanC2 E. casselflavus were isolated. These showed low-level resistance to vancomycin and susceptibility to teicoplanin. However, 9 VRE strains from raw chicken meat had high-level resistance to vancomycin (MIC50,90: > 128 μg/mL), teicoplanin (MIC50,90: > 128 μg/mL), ampicillin (MIC50:,90: > 128 μg/mL), erythromycin (MIC50,90: > 128 μg/mL), and tetracycline (MIC50/90: 128/>128 μg/mL). Conclusion: This study demonstrated little evidence of VRE colonization in healthy people despite high recovery of VRE among raw chicken meat. It is suggested that there is little evidence of VRE transmission from animals to healthy people. However, we assumed that there exists the possibility of VRE contamination during the processing of chicken meat.

KW - Domestic animals

KW - Healthy person

KW - PCR

KW - PFGE (electrophoresis, gel, pulsed-field)

KW - Vancomycin-resistant enterococcus

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