PREVENTION OF EVAPORATION IN FEMTOLITER-SCALE WATER-IN-AIR DROPLET GENERATION FOR DIGITAL COUNTING OF BIOMOLECULES

Bo Hoon Han, Juhwan Park, Seok Chung, Ji Yoon Kang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The major challenge of digital counting using air-water interface droplet array is the prevention of evaporation. In this study, we generated the 100,000 droplets in a few seconds by passing the liquid plug to hydrophilic/hydrophobic array for digital counting of biomolecules. The evaporation of 30 fL-scale droplet array could be delayed from 2 seconds to more than 10 hours based on the simple clamping system and inversion of the device. Finally, digital enzyme linked immunosorbent assay (dELISA) was performed to verify the possibility of application of biochemical assay, and amyloid beta protein was quantified as a proof of concept.

Original languageEnglish
Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages821-822
Number of pages2
ISBN (Electronic)9781733419031
Publication statusPublished - 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
Duration: 2021 Oct 102021 Oct 14

Publication series

NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs, Virtual
Period21/10/1021/10/14

Keywords

  • Air-water interface droplet
  • Digital ELISA
  • Droplet array
  • Prevention of evaporation

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

Fingerprint

Dive into the research topics of 'PREVENTION OF EVAPORATION IN FEMTOLITER-SCALE WATER-IN-AIR DROPLET GENERATION FOR DIGITAL COUNTING OF BIOMOLECULES'. Together they form a unique fingerprint.

Cite this