TY - GEN
T1 - PREVENTION OF EVAPORATION IN FEMTOLITER-SCALE WATER-IN-AIR DROPLET GENERATION FOR DIGITAL COUNTING OF BIOMOLECULES
AU - Han, Bo Hoon
AU - Park, Juhwan
AU - Chung, Seok
AU - Kang, Ji Yoon
N1 - Funding Information:
This research was supported mainly by the Nano-material Technology Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017M3A7B4049851). It was also supported by the (KMDF_PR_20200901_0072) from the Korea Medical Device Development Fund (KMDF).
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - The major challenge of digital counting using air-water interface droplet array is the prevention of evaporation. In this study, we generated the 100,000 droplets in a few seconds by passing the liquid plug to hydrophilic/hydrophobic array for digital counting of biomolecules. The evaporation of 30 fL-scale droplet array could be delayed from 2 seconds to more than 10 hours based on the simple clamping system and inversion of the device. Finally, digital enzyme linked immunosorbent assay (dELISA) was performed to verify the possibility of application of biochemical assay, and amyloid beta protein was quantified as a proof of concept.
AB - The major challenge of digital counting using air-water interface droplet array is the prevention of evaporation. In this study, we generated the 100,000 droplets in a few seconds by passing the liquid plug to hydrophilic/hydrophobic array for digital counting of biomolecules. The evaporation of 30 fL-scale droplet array could be delayed from 2 seconds to more than 10 hours based on the simple clamping system and inversion of the device. Finally, digital enzyme linked immunosorbent assay (dELISA) was performed to verify the possibility of application of biochemical assay, and amyloid beta protein was quantified as a proof of concept.
KW - Air-water interface droplet
KW - Digital ELISA
KW - Droplet array
KW - Prevention of evaporation
UR - http://www.scopus.com/inward/record.url?scp=85136983663&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136983663
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 821
EP - 822
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -