Preventive effects of Rosa rugosa root extract on advanced glycation end product-induced endothelial dysfunction

Mi Hyun Nam, Hyun Sun Lee, Chung Oui Hong, Yoon Chang Koo, Young Seomun, Kwang Won Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Rosa rugosa has traditionally been used as a folk remedy for diabetes. The objective of this study was therefore to demonstrate the inhibition of endothelial dysfunction activities through antioxidants and the anti-glycation of Rosa rugosa roots. Dried roots of Rosa rugosa were boiled in methanol for three hours, evaporated and lyophilized with a freeze-dryer. The methanolic extract of Rosa rugosa roots (RRE) was tested for antioxidant activities by measuring total polyphenol (TP) content, flavonoid content, 1,1-diphenyl-2- picrylhydrazyl free radical-scavenging activity (DPPH) assay, and ferric-reducing antioxidant power (FRAP) assay. The total TP content, flavonoid content, FRAP value, and DPPH-SC50 are 345.2 μg gallic acid equivalents/mg dry matter (DM), 128.1 μg quercetin equivalents/mg DM, 2.2 mM FeSO4/mg DM and 34.2 μg DM/mL, respectively. Treatment of RRE significantly lowered fluorescent formation due to advanced glycation reaction. In addition, reactive oxygen species (ROS) scavenging assay, monocyte adherent assay and transendothelial electrical resistance (TEER) assay were performed to investigate the possibility that RRE improves endothelial dysfunction-induced diabetic complications. The adhesion of THP-1 to treated HUVEC with RRE (100 μg/mL; 33% and 500 μg/mL; 75%) was significantly reduced compared to HUVEC stimulated by glyceraldehydes-AGEs (advanced glycation end product). The TEER value (88 Ω·cm2) of stimulated HUVEC by glyceraldehydes-AGEs was reduced compared to non-stimulation (113 Ω·cm2). However, normalization with RRE increased endothelial permeability in a dose-dependent manner (100 μg/mL; 102 Ω·cm2 and 500 μg/mL; 106 Ω·cm 2). Thus, these results suggest that Rosa rugosa roots could be a novel candidate for the prevention of diabetic complications through antioxidants and inhibition of advanced glycation end product formation.

Original languageEnglish
Pages (from-to)210-216
Number of pages7
JournalKorean Journal of Food Science and Technology
Volume42
Issue number2
Publication statusPublished - 2010 Dec 1

Fingerprint

Rosa rugosa
Advanced Glycosylation End Products
Antioxidants
glyceraldehyde
Glyceraldehyde
extracts
assays
glycation
electrical resistance
Polyphenols
Diabetes Complications
Electric Impedance
antioxidants
Flavonoids
polyphenols
freeze dryers
flavonoids
antioxidant activity
Gallic Acid
Quercetin

Keywords

  • Advanced glycation end product
  • Antioxidant activities
  • Endothelial dysfunction
  • Monocyte adherent assay
  • Rosa rugosa root
  • TEER

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Preventive effects of Rosa rugosa root extract on advanced glycation end product-induced endothelial dysfunction. / Nam, Mi Hyun; Lee, Hyun Sun; Hong, Chung Oui; Koo, Yoon Chang; Seomun, Young; Lee, Kwang Won.

In: Korean Journal of Food Science and Technology, Vol. 42, No. 2, 01.12.2010, p. 210-216.

Research output: Contribution to journalArticle

Nam, Mi Hyun ; Lee, Hyun Sun ; Hong, Chung Oui ; Koo, Yoon Chang ; Seomun, Young ; Lee, Kwang Won. / Preventive effects of Rosa rugosa root extract on advanced glycation end product-induced endothelial dysfunction. In: Korean Journal of Food Science and Technology. 2010 ; Vol. 42, No. 2. pp. 210-216.
@article{d8584d53d87a4f0ca1925e39de38d2fb,
title = "Preventive effects of Rosa rugosa root extract on advanced glycation end product-induced endothelial dysfunction",
abstract = "Rosa rugosa has traditionally been used as a folk remedy for diabetes. The objective of this study was therefore to demonstrate the inhibition of endothelial dysfunction activities through antioxidants and the anti-glycation of Rosa rugosa roots. Dried roots of Rosa rugosa were boiled in methanol for three hours, evaporated and lyophilized with a freeze-dryer. The methanolic extract of Rosa rugosa roots (RRE) was tested for antioxidant activities by measuring total polyphenol (TP) content, flavonoid content, 1,1-diphenyl-2- picrylhydrazyl free radical-scavenging activity (DPPH) assay, and ferric-reducing antioxidant power (FRAP) assay. The total TP content, flavonoid content, FRAP value, and DPPH-SC50 are 345.2 μg gallic acid equivalents/mg dry matter (DM), 128.1 μg quercetin equivalents/mg DM, 2.2 mM FeSO4/mg DM and 34.2 μg DM/mL, respectively. Treatment of RRE significantly lowered fluorescent formation due to advanced glycation reaction. In addition, reactive oxygen species (ROS) scavenging assay, monocyte adherent assay and transendothelial electrical resistance (TEER) assay were performed to investigate the possibility that RRE improves endothelial dysfunction-induced diabetic complications. The adhesion of THP-1 to treated HUVEC with RRE (100 μg/mL; 33{\%} and 500 μg/mL; 75{\%}) was significantly reduced compared to HUVEC stimulated by glyceraldehydes-AGEs (advanced glycation end product). The TEER value (88 Ω·cm2) of stimulated HUVEC by glyceraldehydes-AGEs was reduced compared to non-stimulation (113 Ω·cm2). However, normalization with RRE increased endothelial permeability in a dose-dependent manner (100 μg/mL; 102 Ω·cm2 and 500 μg/mL; 106 Ω·cm 2). Thus, these results suggest that Rosa rugosa roots could be a novel candidate for the prevention of diabetic complications through antioxidants and inhibition of advanced glycation end product formation.",
keywords = "Advanced glycation end product, Antioxidant activities, Endothelial dysfunction, Monocyte adherent assay, Rosa rugosa root, TEER",
author = "Nam, {Mi Hyun} and Lee, {Hyun Sun} and Hong, {Chung Oui} and Koo, {Yoon Chang} and Young Seomun and Lee, {Kwang Won}",
year = "2010",
month = "12",
day = "1",
language = "English",
volume = "42",
pages = "210--216",
journal = "Korean Journal of Food Science and Technology",
issn = "0367-6293",
publisher = "Tong Kwahak Hoe",
number = "2",

}

TY - JOUR

T1 - Preventive effects of Rosa rugosa root extract on advanced glycation end product-induced endothelial dysfunction

AU - Nam, Mi Hyun

AU - Lee, Hyun Sun

AU - Hong, Chung Oui

AU - Koo, Yoon Chang

AU - Seomun, Young

AU - Lee, Kwang Won

PY - 2010/12/1

Y1 - 2010/12/1

N2 - Rosa rugosa has traditionally been used as a folk remedy for diabetes. The objective of this study was therefore to demonstrate the inhibition of endothelial dysfunction activities through antioxidants and the anti-glycation of Rosa rugosa roots. Dried roots of Rosa rugosa were boiled in methanol for three hours, evaporated and lyophilized with a freeze-dryer. The methanolic extract of Rosa rugosa roots (RRE) was tested for antioxidant activities by measuring total polyphenol (TP) content, flavonoid content, 1,1-diphenyl-2- picrylhydrazyl free radical-scavenging activity (DPPH) assay, and ferric-reducing antioxidant power (FRAP) assay. The total TP content, flavonoid content, FRAP value, and DPPH-SC50 are 345.2 μg gallic acid equivalents/mg dry matter (DM), 128.1 μg quercetin equivalents/mg DM, 2.2 mM FeSO4/mg DM and 34.2 μg DM/mL, respectively. Treatment of RRE significantly lowered fluorescent formation due to advanced glycation reaction. In addition, reactive oxygen species (ROS) scavenging assay, monocyte adherent assay and transendothelial electrical resistance (TEER) assay were performed to investigate the possibility that RRE improves endothelial dysfunction-induced diabetic complications. The adhesion of THP-1 to treated HUVEC with RRE (100 μg/mL; 33% and 500 μg/mL; 75%) was significantly reduced compared to HUVEC stimulated by glyceraldehydes-AGEs (advanced glycation end product). The TEER value (88 Ω·cm2) of stimulated HUVEC by glyceraldehydes-AGEs was reduced compared to non-stimulation (113 Ω·cm2). However, normalization with RRE increased endothelial permeability in a dose-dependent manner (100 μg/mL; 102 Ω·cm2 and 500 μg/mL; 106 Ω·cm 2). Thus, these results suggest that Rosa rugosa roots could be a novel candidate for the prevention of diabetic complications through antioxidants and inhibition of advanced glycation end product formation.

AB - Rosa rugosa has traditionally been used as a folk remedy for diabetes. The objective of this study was therefore to demonstrate the inhibition of endothelial dysfunction activities through antioxidants and the anti-glycation of Rosa rugosa roots. Dried roots of Rosa rugosa were boiled in methanol for three hours, evaporated and lyophilized with a freeze-dryer. The methanolic extract of Rosa rugosa roots (RRE) was tested for antioxidant activities by measuring total polyphenol (TP) content, flavonoid content, 1,1-diphenyl-2- picrylhydrazyl free radical-scavenging activity (DPPH) assay, and ferric-reducing antioxidant power (FRAP) assay. The total TP content, flavonoid content, FRAP value, and DPPH-SC50 are 345.2 μg gallic acid equivalents/mg dry matter (DM), 128.1 μg quercetin equivalents/mg DM, 2.2 mM FeSO4/mg DM and 34.2 μg DM/mL, respectively. Treatment of RRE significantly lowered fluorescent formation due to advanced glycation reaction. In addition, reactive oxygen species (ROS) scavenging assay, monocyte adherent assay and transendothelial electrical resistance (TEER) assay were performed to investigate the possibility that RRE improves endothelial dysfunction-induced diabetic complications. The adhesion of THP-1 to treated HUVEC with RRE (100 μg/mL; 33% and 500 μg/mL; 75%) was significantly reduced compared to HUVEC stimulated by glyceraldehydes-AGEs (advanced glycation end product). The TEER value (88 Ω·cm2) of stimulated HUVEC by glyceraldehydes-AGEs was reduced compared to non-stimulation (113 Ω·cm2). However, normalization with RRE increased endothelial permeability in a dose-dependent manner (100 μg/mL; 102 Ω·cm2 and 500 μg/mL; 106 Ω·cm 2). Thus, these results suggest that Rosa rugosa roots could be a novel candidate for the prevention of diabetic complications through antioxidants and inhibition of advanced glycation end product formation.

KW - Advanced glycation end product

KW - Antioxidant activities

KW - Endothelial dysfunction

KW - Monocyte adherent assay

KW - Rosa rugosa root

KW - TEER

UR - http://www.scopus.com/inward/record.url?scp=79959452531&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959452531&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:79959452531

VL - 42

SP - 210

EP - 216

JO - Korean Journal of Food Science and Technology

JF - Korean Journal of Food Science and Technology

SN - 0367-6293

IS - 2

ER -