Abstract
Rosa rugosa has traditionally been used as a folk remedy for diabetes. The objective of this study was therefore to demonstrate the inhibition of endothelial dysfunction activities through antioxidants and the anti-glycation of Rosa rugosa roots. Dried roots of Rosa rugosa were boiled in methanol for three hours, evaporated and lyophilized with a freeze-dryer. The methanolic extract of Rosa rugosa roots (RRE) was tested for antioxidant activities by measuring total polyphenol (TP) content, flavonoid content, 1,1-diphenyl-2- picrylhydrazyl free radical-scavenging activity (DPPH) assay, and ferric-reducing antioxidant power (FRAP) assay. The total TP content, flavonoid content, FRAP value, and DPPH-SC50 are 345.2 μg gallic acid equivalents/mg dry matter (DM), 128.1 μg quercetin equivalents/mg DM, 2.2 mM FeSO4/mg DM and 34.2 μg DM/mL, respectively. Treatment of RRE significantly lowered fluorescent formation due to advanced glycation reaction. In addition, reactive oxygen species (ROS) scavenging assay, monocyte adherent assay and transendothelial electrical resistance (TEER) assay were performed to investigate the possibility that RRE improves endothelial dysfunction-induced diabetic complications. The adhesion of THP-1 to treated HUVEC with RRE (100 μg/mL; 33% and 500 μg/mL; 75%) was significantly reduced compared to HUVEC stimulated by glyceraldehydes-AGEs (advanced glycation end product). The TEER value (88 Ω·cm2) of stimulated HUVEC by glyceraldehydes-AGEs was reduced compared to non-stimulation (113 Ω·cm2). However, normalization with RRE increased endothelial permeability in a dose-dependent manner (100 μg/mL; 102 Ω·cm2 and 500 μg/mL; 106 Ω·cm 2). Thus, these results suggest that Rosa rugosa roots could be a novel candidate for the prevention of diabetic complications through antioxidants and inhibition of advanced glycation end product formation.
| Original language | English |
|---|---|
| Pages (from-to) | 210-216 |
| Number of pages | 7 |
| Journal | Korean Journal of Food Science and Technology |
| Volume | 42 |
| Issue number | 2 |
| Publication status | Published - 2010 Dec 1 |
Fingerprint
Keywords
- Advanced glycation end product
- Antioxidant activities
- Endothelial dysfunction
- Monocyte adherent assay
- Rosa rugosa root
- TEER
ASJC Scopus subject areas
- Food Science
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Preventive effects of Rosa rugosa root extract on advanced glycation end product-induced endothelial dysfunction. / Nam, Mi Hyun; Lee, Hyun Sun; Hong, Chung Oui; Koo, Yoon Chang; Seomun, Young; Lee, Kwang Won.
In: Korean Journal of Food Science and Technology, Vol. 42, No. 2, 01.12.2010, p. 210-216.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Preventive effects of Rosa rugosa root extract on advanced glycation end product-induced endothelial dysfunction
AU - Nam, Mi Hyun
AU - Lee, Hyun Sun
AU - Hong, Chung Oui
AU - Koo, Yoon Chang
AU - Seomun, Young
AU - Lee, Kwang Won
PY - 2010/12/1
Y1 - 2010/12/1
N2 - Rosa rugosa has traditionally been used as a folk remedy for diabetes. The objective of this study was therefore to demonstrate the inhibition of endothelial dysfunction activities through antioxidants and the anti-glycation of Rosa rugosa roots. Dried roots of Rosa rugosa were boiled in methanol for three hours, evaporated and lyophilized with a freeze-dryer. The methanolic extract of Rosa rugosa roots (RRE) was tested for antioxidant activities by measuring total polyphenol (TP) content, flavonoid content, 1,1-diphenyl-2- picrylhydrazyl free radical-scavenging activity (DPPH) assay, and ferric-reducing antioxidant power (FRAP) assay. The total TP content, flavonoid content, FRAP value, and DPPH-SC50 are 345.2 μg gallic acid equivalents/mg dry matter (DM), 128.1 μg quercetin equivalents/mg DM, 2.2 mM FeSO4/mg DM and 34.2 μg DM/mL, respectively. Treatment of RRE significantly lowered fluorescent formation due to advanced glycation reaction. In addition, reactive oxygen species (ROS) scavenging assay, monocyte adherent assay and transendothelial electrical resistance (TEER) assay were performed to investigate the possibility that RRE improves endothelial dysfunction-induced diabetic complications. The adhesion of THP-1 to treated HUVEC with RRE (100 μg/mL; 33% and 500 μg/mL; 75%) was significantly reduced compared to HUVEC stimulated by glyceraldehydes-AGEs (advanced glycation end product). The TEER value (88 Ω·cm2) of stimulated HUVEC by glyceraldehydes-AGEs was reduced compared to non-stimulation (113 Ω·cm2). However, normalization with RRE increased endothelial permeability in a dose-dependent manner (100 μg/mL; 102 Ω·cm2 and 500 μg/mL; 106 Ω·cm 2). Thus, these results suggest that Rosa rugosa roots could be a novel candidate for the prevention of diabetic complications through antioxidants and inhibition of advanced glycation end product formation.
AB - Rosa rugosa has traditionally been used as a folk remedy for diabetes. The objective of this study was therefore to demonstrate the inhibition of endothelial dysfunction activities through antioxidants and the anti-glycation of Rosa rugosa roots. Dried roots of Rosa rugosa were boiled in methanol for three hours, evaporated and lyophilized with a freeze-dryer. The methanolic extract of Rosa rugosa roots (RRE) was tested for antioxidant activities by measuring total polyphenol (TP) content, flavonoid content, 1,1-diphenyl-2- picrylhydrazyl free radical-scavenging activity (DPPH) assay, and ferric-reducing antioxidant power (FRAP) assay. The total TP content, flavonoid content, FRAP value, and DPPH-SC50 are 345.2 μg gallic acid equivalents/mg dry matter (DM), 128.1 μg quercetin equivalents/mg DM, 2.2 mM FeSO4/mg DM and 34.2 μg DM/mL, respectively. Treatment of RRE significantly lowered fluorescent formation due to advanced glycation reaction. In addition, reactive oxygen species (ROS) scavenging assay, monocyte adherent assay and transendothelial electrical resistance (TEER) assay were performed to investigate the possibility that RRE improves endothelial dysfunction-induced diabetic complications. The adhesion of THP-1 to treated HUVEC with RRE (100 μg/mL; 33% and 500 μg/mL; 75%) was significantly reduced compared to HUVEC stimulated by glyceraldehydes-AGEs (advanced glycation end product). The TEER value (88 Ω·cm2) of stimulated HUVEC by glyceraldehydes-AGEs was reduced compared to non-stimulation (113 Ω·cm2). However, normalization with RRE increased endothelial permeability in a dose-dependent manner (100 μg/mL; 102 Ω·cm2 and 500 μg/mL; 106 Ω·cm 2). Thus, these results suggest that Rosa rugosa roots could be a novel candidate for the prevention of diabetic complications through antioxidants and inhibition of advanced glycation end product formation.
KW - Advanced glycation end product
KW - Antioxidant activities
KW - Endothelial dysfunction
KW - Monocyte adherent assay
KW - Rosa rugosa root
KW - TEER
UR - http://www.scopus.com/inward/record.url?scp=79959452531&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79959452531&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:79959452531
VL - 42
SP - 210
EP - 216
JO - Korean Journal of Food Science and Technology
JF - Korean Journal of Food Science and Technology
SN - 0367-6293
IS - 2
ER -