Primed monocytes

Putative culprits of chronic low-grade inflammation and impaired innate immune responses in patients on hemodialysis

Hye Won Kim, Young Seok Woo, Ha Na Yang, Hye Min Choi, Sang Kyung Jo, Won Yong Cho, Hyoung Kyu Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: End-stage renal disease patients are known to be in a state of chronic low-grade inflammation and to have high infection-related morbidity and mortality. However, the precise mechanisms are not understood. The purpose of this study was to determine the mechanisms underlying chronic low-grade inflammation and defects in innate immune responses in hemodialysis (HD) patients. Method: In 33 HD patients, we measured the basal status of toll-like receptor 4 (TLR4) positivity in the CD14-positive monocyte population in the peripheral blood (not strong, i.e., CD14low), with plasma levels of tumor necrosis factor (TNF)-α and interleukin (IL)-1β, IL-6, IL-8, IL-10, and IL-12p70 compared with 22 healthy controls. After stimulation by lipopolysaccharide (LPS), the plasma cytokine response was also compared. Results: In the basal state, the percentage of peripheral blood TLR4 +CD14low monocytes and plasma cytokines were significantly higher in HD patients (p<0.05), suggesting that preactivated primed monocytes might be responsible for the chronic inflammatory state in HD patients. However, upon LPS challenge, the fold increase in plasma cytokine response was significantly reduced in HD patients (p<0.05) compared with controls. More importantly, the fold increase of these cytokines showed a positive correlation with plasma albumin (p<0.05) and a negative correlation with C-reactive protein (CRP) (p<0.05), suggesting the presence of a possible link between chronic low-grade inflammation and suboptimal innate immune response. Conclusion: Chronic low-grade inflammation due to preactivated peripheral blood CD14+ leukocyte subset might be a mechanism for impaired innate immune responses, thus resulting in the high rates of infection-related morbidity and mortality observed in HD patients.

Original languageEnglish
Pages (from-to)258-263
Number of pages6
JournalClinical and Experimental Nephrology
Volume15
Issue number2
DOIs
Publication statusPublished - 2011 Apr 1

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Innate Immunity
Renal Dialysis
Monocytes
Inflammation
Cytokines
Toll-Like Receptor 4
Lipopolysaccharides
Morbidity
Mortality
Interleukins
Infection
Interleukin-8
Interleukin-1
Serum Albumin
Interleukin-10
C-Reactive Protein
Chronic Kidney Failure
Interleukin-6
Leukocytes
Tumor Necrosis Factor-alpha

Keywords

  • Hemodialysis
  • Inflammation
  • Monocytes
  • Toll-like receptor 4

ASJC Scopus subject areas

  • Physiology
  • Nephrology
  • Physiology (medical)

Cite this

Primed monocytes : Putative culprits of chronic low-grade inflammation and impaired innate immune responses in patients on hemodialysis. / Kim, Hye Won; Woo, Young Seok; Yang, Ha Na; Choi, Hye Min; Jo, Sang Kyung; Cho, Won Yong; Kim, Hyoung Kyu.

In: Clinical and Experimental Nephrology, Vol. 15, No. 2, 01.04.2011, p. 258-263.

Research output: Contribution to journalArticle

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AU - Choi, Hye Min

AU - Jo, Sang Kyung

AU - Cho, Won Yong

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