Priming-mediated systemic resistance in cucumber induced by Pseudomonas azotoformans GC-B19 and Paenibacillus elgii MM-B22 against colletotrichum orbiculare

Mee Kyung Sang, Eui Nam Kim, Gyung Deok Han, Min Sun Kwack, Yong Chull Jeun, Ki Deok Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Induced systemic resistance (ISR) can be activated by biotic agents, including root-associated beneficial bacteria to inhibit pathogen infection. We investigated priming-mediated ISR in cucumber induced by Pseudomonas azotoformans GC-B19 and Paenibacillus elgii MM-B22 against Colletotrichum orbiculare (causal fungus of anthracnose). In addition, we examined whether this ISR expression was bacterial density-dependent by assessing peroxidase activity in the presence and absence of the pathogen. As a result, root treatment with the ISR-eliciting strains GCB19 and MM-B22 or the chemical inducer DL-ß-amino-n-butyric acid (positive control) significantly inhibited fungal infection process (conidial germination and appressorium formation) and disease severity compared with the non-ISR-eliciting strain, Pseudomonas aeruginosa PK-B09 (negative control), and MgSO4 solution (untreated control). These treatments effectively induced rapid elicitation of hypersensitive reaction-like cell death with H2O2 generations, and accumulation of defense-related enzymes (ß-1,3-glucanase, chitinase, and peroxidase) in cucumber leaves in the "primed" state against C. orbiculare. In addition, ISR expression was dependent on the bacterial cell density in the rhizosphere. This ISR expression was derived from the presence of sustained bacterial populations ranging from 104 to 106 cells/g of potting mix over a period of time after introduction of bacteria (106 to 1010 cells/g of potting mix) into the rhizosphere. Taken together, these results suggest that priming-mediated ISR against C. orbiculare in cucumber can be induced in a bacterial density-dependent manner by Pseudomonas azotoformans GC-B19 and Paenibacillus elgii MM-B22.

Original languageEnglish
Pages (from-to)834-842
Number of pages9
JournalPhytopathology
Volume104
Issue number8
DOIs
Publication statusPublished - 2014 Jan 1

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Pseudomonas azotoformans
Paenibacillus
Colletotrichum orbiculare
Colletotrichum
Cucumis sativus
Pseudomonas
cucumbers
Rhizosphere
Peroxidase
rhizosphere
peroxidase
Bacteria
Chitinases
appressoria
Butyric Acid
Mycoses
pathogens
bacteria
butyric acid
anthracnose

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Priming-mediated systemic resistance in cucumber induced by Pseudomonas azotoformans GC-B19 and Paenibacillus elgii MM-B22 against colletotrichum orbiculare. / Sang, Mee Kyung; Kim, Eui Nam; Han, Gyung Deok; Kwack, Min Sun; Jeun, Yong Chull; Kim, Ki Deok.

In: Phytopathology, Vol. 104, No. 8, 01.01.2014, p. 834-842.

Research output: Contribution to journalArticle

Sang, Mee Kyung ; Kim, Eui Nam ; Han, Gyung Deok ; Kwack, Min Sun ; Jeun, Yong Chull ; Kim, Ki Deok. / Priming-mediated systemic resistance in cucumber induced by Pseudomonas azotoformans GC-B19 and Paenibacillus elgii MM-B22 against colletotrichum orbiculare. In: Phytopathology. 2014 ; Vol. 104, No. 8. pp. 834-842.
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AU - Sang, Mee Kyung

AU - Kim, Eui Nam

AU - Han, Gyung Deok

AU - Kwack, Min Sun

AU - Jeun, Yong Chull

AU - Kim, Ki Deok

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AB - Induced systemic resistance (ISR) can be activated by biotic agents, including root-associated beneficial bacteria to inhibit pathogen infection. We investigated priming-mediated ISR in cucumber induced by Pseudomonas azotoformans GC-B19 and Paenibacillus elgii MM-B22 against Colletotrichum orbiculare (causal fungus of anthracnose). In addition, we examined whether this ISR expression was bacterial density-dependent by assessing peroxidase activity in the presence and absence of the pathogen. As a result, root treatment with the ISR-eliciting strains GCB19 and MM-B22 or the chemical inducer DL-ß-amino-n-butyric acid (positive control) significantly inhibited fungal infection process (conidial germination and appressorium formation) and disease severity compared with the non-ISR-eliciting strain, Pseudomonas aeruginosa PK-B09 (negative control), and MgSO4 solution (untreated control). These treatments effectively induced rapid elicitation of hypersensitive reaction-like cell death with H2O2 generations, and accumulation of defense-related enzymes (ß-1,3-glucanase, chitinase, and peroxidase) in cucumber leaves in the "primed" state against C. orbiculare. In addition, ISR expression was dependent on the bacterial cell density in the rhizosphere. This ISR expression was derived from the presence of sustained bacterial populations ranging from 104 to 106 cells/g of potting mix over a period of time after introduction of bacteria (106 to 1010 cells/g of potting mix) into the rhizosphere. Taken together, these results suggest that priming-mediated ISR against C. orbiculare in cucumber can be induced in a bacterial density-dependent manner by Pseudomonas azotoformans GC-B19 and Paenibacillus elgii MM-B22.

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KW - Reactive oxygen species

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