Probiotic potential of enterococcus faecium isolated from chicken cecum with immunomodulating activity and promoting longevity in caenorhabditis elegans

Insuk Sim, Keun Tae Park, Gayeung Kwon, Jong Ho Koh, Young Hee Lim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Probiotics, including Enterococcus faecium, confer a health benefit on the host. An Enterococcus strain was isolated from healthy chicken cecum, identified as E. faecium by 16S rDNA gene sequence analysis, and designated as E. faecium L11. To evaluate the potential of E. faecium L11 as a probiotic, the gastrointestinal tolerance, immunomodulatory activity, and lifespan extension properties of the strain were assayed. E. faecium L11 showed >66% and >62% survival in artificial gastric juice (0.3% pepsin, pH 2.5) and simulated small intestinal juice (0.5% bile salt and 0.1% pancreatin), respectively. Heat-killed E. faecium L11 significantly (p < 0.05) increased immune cell proliferation compared with controls, and stimulated the production of cytokines (IL-6 and TNF-α) by activated macrophages obtained from ICR mice. In addition, E. faecium L11 showed a protective effect against Salmonella Typhimurium infection in Caenorhabditis elegans. In addition, feeding E. faecium L11 significantly (p < 0.05) extended the lifespan of C. elegans compared with the control. Furthermore, genes related to aging and host defense were upregulated in E. faecium L11-fed worms. In conclusion, E. faecium L11, which prolongs the lifespan of C. elegans, may be a potent probiotic supplement for livestock.

Original languageEnglish
Pages (from-to)883-892
Number of pages10
JournalJournal of Microbiology and Biotechnology
Volume28
Issue number6
DOIs
Publication statusPublished - 2018 Jun 1

Fingerprint

Enterococcus faecium
Cecum
Caenorhabditis elegans
Probiotics
Chickens
Pancreatin
Inbred ICR Mouse
Gastric Juice
Salmonella Infections
Pepsin A
Enterococcus
Livestock
Insurance Benefits
Salmonella typhimurium
Ribosomal DNA
Bile Acids and Salts
Genes
Sequence Analysis
Interleukin-6
Hot Temperature

Keywords

  • Caenorhabditis elegans
  • Enterococcus faecium
  • Immunomodulatory activity
  • Lifespan extension
  • Probiotics

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Probiotic potential of enterococcus faecium isolated from chicken cecum with immunomodulating activity and promoting longevity in caenorhabditis elegans. / Sim, Insuk; Park, Keun Tae; Kwon, Gayeung; Koh, Jong Ho; Lim, Young Hee.

In: Journal of Microbiology and Biotechnology, Vol. 28, No. 6, 01.06.2018, p. 883-892.

Research output: Contribution to journalArticle

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