Probiotic potential of Staphylococcus hominis MBBL 2-9 as anti-Staphylococcus aureus agent isolated from the vaginal microbiota of a healthy woman

C. Sung, B. G. Kim, Sae Hun Kim, H. S. Joo, P. I. Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Aims: To isolate and characterize an antagonist for use as probiotic agent in the biocontrol of Staphylococcus aureus. Methods and Results: Bacteria that exhibited antimicrobial activity against Gram-positive bacteria including Staph. aureus were isolated from 12 healthy women, with Staphylococcus hominis MBBL 2-9 showing the strongest activity. The bacteriocin produced by Staph. hominis MBBL 2-9 was purified by 60% ammonium sulfate saturation, ultrafiltration, HLB cartridge and reverse-phase HPLC. The molecular weight was estimated as 2038·2 Da by MALDI-TOF mass spectrometry. The antagonist survived up to 2 h in artificial gastric juice (pH 2·5) and grew in the presence of 1% porcine bile extract. In addition, Staph. hominis MBBL 2-9 adhered effectively to HT-29 epithelial cell line. Conclusion: Staphylococcus hominis MBBL 2-9 exhibited desirable probiotic traits such as acid tolerance, bile resistance and adherence to epithelial cell line. The bacterium also produced a bacteriocin with unique molecular weight and high antimicrobial activity similar to traditional antibiotics. Significance and Impact of the Study: This study is the first report of a bacteriocin-producing Staph. hominis MBBL 2-9 that has potential for use as a probiotic agent against Staph. aureus.

Original languageEnglish
Pages (from-to)908-916
Number of pages9
JournalJournal of Applied Microbiology
Volume108
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1

Fingerprint

Staphylococcus hominis
Bacteriocins
Microbiota
Probiotics
Staphylococcus aureus
Molecular Weight
Epithelial Cells
Bacteria
Cell Line
HT29 Cells
Gastric Juice
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Ultrafiltration
Ammonium Sulfate
Gram-Positive Bacteria
Bile Acids and Salts
Bile
Mass Spectrometry
Swine
High Pressure Liquid Chromatography

Keywords

  • Antagonist
  • Bacteriocin
  • Biocontrol
  • Probiotics
  • Staphylococcus hominis MBBL 2-9

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology

Cite this

Probiotic potential of Staphylococcus hominis MBBL 2-9 as anti-Staphylococcus aureus agent isolated from the vaginal microbiota of a healthy woman. / Sung, C.; Kim, B. G.; Kim, Sae Hun; Joo, H. S.; Kim, P. I.

In: Journal of Applied Microbiology, Vol. 108, No. 3, 01.03.2010, p. 908-916.

Research output: Contribution to journalArticle

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