Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract

Pyoung I. Kim, Min Young Jung, Young H. Chang, Sae Hun Kim, Seong J. Kim, Yong H. Park

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56 Citations (Scopus)

Abstract

One strain of Lactobacillus salivarius, two strains of Lactobacillus reuteri and Lactobacillus amylovorus, and two strains of Bifidobacterium thermacidophilum with antagonistic effect against Clostridium perfringens were isolated from porcine gastrointestinal tract. Isolates were assayed for their ability to survive in synthetic gastric juice at pH 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. There was a large variation in the survival of the isolates in gastric juice and growth in the medium containing 0.3% (w/v) bile. L. salivarius G11 and L. amylovorus S6 adhered to the HT-29 epithelial cell line. Cell-free supernatant of L. amylovorus S6 showed higher antagonistic activity as effective as the antibiotics such as neomycin, chlortetracycline, and oxytetracycline against bacterial pathogens including C. perfringens, Salmonella typhimurium, Staphylococcus aureus, Vibrio cholerae, Edwardsiella tarda, and Aeromonas salmonicida subsp. salmonicida.

Original languageEnglish
Pages (from-to)1103-1111
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume74
Issue number5
DOIs
Publication statusPublished - 2007 Apr 1

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Keywords

  • Antagonistic effect
  • Bifidobacterium thermacidophilum
  • Clostridium perfringens
  • Lactobacillus amylovorus
  • Lactobacillus reuteri
  • Lactobacillus salivarius

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Microbiology
  • Microbiology (medical)

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