Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract

Pyoung I. Kim, Min Young Jung, Young H. Chang, Sae Hun Kim, Seong J. Kim, Yong H. Park

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

One strain of Lactobacillus salivarius, two strains of Lactobacillus reuteri and Lactobacillus amylovorus, and two strains of Bifidobacterium thermacidophilum with antagonistic effect against Clostridium perfringens were isolated from porcine gastrointestinal tract. Isolates were assayed for their ability to survive in synthetic gastric juice at pH 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. There was a large variation in the survival of the isolates in gastric juice and growth in the medium containing 0.3% (w/v) bile. L. salivarius G11 and L. amylovorus S6 adhered to the HT-29 epithelial cell line. Cell-free supernatant of L. amylovorus S6 showed higher antagonistic activity as effective as the antibiotics such as neomycin, chlortetracycline, and oxytetracycline against bacterial pathogens including C. perfringens, Salmonella typhimurium, Staphylococcus aureus, Vibrio cholerae, Edwardsiella tarda, and Aeromonas salmonicida subsp. salmonicida.

Original languageEnglish
Pages (from-to)1103-1111
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume74
Issue number5
DOIs
Publication statusPublished - 2007 Apr 1

Fingerprint

Lactobacillus acidophilus
Chlortetracycline
Clostridium
Oxytetracycline
Salmonella
Bifidobacterium
Neomycin
Probiotics
Lactobacillus
Pathogens
Antibiotics
Agar
Gastrointestinal Tract
S 6
Clostridium perfringens
Gastric Juice
Swine
Anti-Bacterial Agents
Bile
Edwardsiella tarda

Keywords

  • Antagonistic effect
  • Bifidobacterium thermacidophilum
  • Clostridium perfringens
  • Lactobacillus amylovorus
  • Lactobacillus reuteri
  • Lactobacillus salivarius

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Microbiology
  • Microbiology (medical)

Cite this

Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract. / Kim, Pyoung I.; Jung, Min Young; Chang, Young H.; Kim, Sae Hun; Kim, Seong J.; Park, Yong H.

In: Applied Microbiology and Biotechnology, Vol. 74, No. 5, 01.04.2007, p. 1103-1111.

Research output: Contribution to journalArticle

Kim, Pyoung I. ; Jung, Min Young ; Chang, Young H. ; Kim, Sae Hun ; Kim, Seong J. ; Park, Yong H. / Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract. In: Applied Microbiology and Biotechnology. 2007 ; Vol. 74, No. 5. pp. 1103-1111.
@article{4c6e172ec7f544b5a963c92e2463409f,
title = "Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract",
abstract = "One strain of Lactobacillus salivarius, two strains of Lactobacillus reuteri and Lactobacillus amylovorus, and two strains of Bifidobacterium thermacidophilum with antagonistic effect against Clostridium perfringens were isolated from porcine gastrointestinal tract. Isolates were assayed for their ability to survive in synthetic gastric juice at pH 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. There was a large variation in the survival of the isolates in gastric juice and growth in the medium containing 0.3{\%} (w/v) bile. L. salivarius G11 and L. amylovorus S6 adhered to the HT-29 epithelial cell line. Cell-free supernatant of L. amylovorus S6 showed higher antagonistic activity as effective as the antibiotics such as neomycin, chlortetracycline, and oxytetracycline against bacterial pathogens including C. perfringens, Salmonella typhimurium, Staphylococcus aureus, Vibrio cholerae, Edwardsiella tarda, and Aeromonas salmonicida subsp. salmonicida.",
keywords = "Antagonistic effect, Bifidobacterium thermacidophilum, Clostridium perfringens, Lactobacillus amylovorus, Lactobacillus reuteri, Lactobacillus salivarius",
author = "Kim, {Pyoung I.} and Jung, {Min Young} and Chang, {Young H.} and Kim, {Sae Hun} and Kim, {Seong J.} and Park, {Yong H.}",
year = "2007",
month = "4",
day = "1",
doi = "10.1007/s00253-006-0741-7",
language = "English",
volume = "74",
pages = "1103--1111",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract

AU - Kim, Pyoung I.

AU - Jung, Min Young

AU - Chang, Young H.

AU - Kim, Sae Hun

AU - Kim, Seong J.

AU - Park, Yong H.

PY - 2007/4/1

Y1 - 2007/4/1

N2 - One strain of Lactobacillus salivarius, two strains of Lactobacillus reuteri and Lactobacillus amylovorus, and two strains of Bifidobacterium thermacidophilum with antagonistic effect against Clostridium perfringens were isolated from porcine gastrointestinal tract. Isolates were assayed for their ability to survive in synthetic gastric juice at pH 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. There was a large variation in the survival of the isolates in gastric juice and growth in the medium containing 0.3% (w/v) bile. L. salivarius G11 and L. amylovorus S6 adhered to the HT-29 epithelial cell line. Cell-free supernatant of L. amylovorus S6 showed higher antagonistic activity as effective as the antibiotics such as neomycin, chlortetracycline, and oxytetracycline against bacterial pathogens including C. perfringens, Salmonella typhimurium, Staphylococcus aureus, Vibrio cholerae, Edwardsiella tarda, and Aeromonas salmonicida subsp. salmonicida.

AB - One strain of Lactobacillus salivarius, two strains of Lactobacillus reuteri and Lactobacillus amylovorus, and two strains of Bifidobacterium thermacidophilum with antagonistic effect against Clostridium perfringens were isolated from porcine gastrointestinal tract. Isolates were assayed for their ability to survive in synthetic gastric juice at pH 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. There was a large variation in the survival of the isolates in gastric juice and growth in the medium containing 0.3% (w/v) bile. L. salivarius G11 and L. amylovorus S6 adhered to the HT-29 epithelial cell line. Cell-free supernatant of L. amylovorus S6 showed higher antagonistic activity as effective as the antibiotics such as neomycin, chlortetracycline, and oxytetracycline against bacterial pathogens including C. perfringens, Salmonella typhimurium, Staphylococcus aureus, Vibrio cholerae, Edwardsiella tarda, and Aeromonas salmonicida subsp. salmonicida.

KW - Antagonistic effect

KW - Bifidobacterium thermacidophilum

KW - Clostridium perfringens

KW - Lactobacillus amylovorus

KW - Lactobacillus reuteri

KW - Lactobacillus salivarius

UR - http://www.scopus.com/inward/record.url?scp=33947271580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947271580&partnerID=8YFLogxK

U2 - 10.1007/s00253-006-0741-7

DO - 10.1007/s00253-006-0741-7

M3 - Article

C2 - 17136367

AN - SCOPUS:33947271580

VL - 74

SP - 1103

EP - 1111

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 5

ER -