TY - JOUR
T1 - Probiotic propionibacterium freudenreichii mj2 enhances osteoblast differentiation and mineralization by increasing the opg/rankl ratio
AU - Yeom, Jiah
AU - Ma, Seongho
AU - Lim, Young Hee
N1 - Funding Information:
Funding: This work was supported by the project for “New business R&D Voucher” between in‐ dustry, academy, and research institutes funded the Korea Ministry of SMEs and Startups in 2019 (No. S2778275).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/4
Y1 - 2021/4
N2 - Osteoblast differentiation is important for the development of bone and the maintenance of bone density. Propionibacterium freudenreichii is a probiotic with an anti‐inflammatory property. The aim of this study was to investigate the enhancement effect of P. freudenreichii MJ2 (MJ2) isolated from raw milk on osteoblast differentiation, mineralization, and its signaling pathway. For in vitro and in vivo experiments, human fetal osteoblastic cell line hFOB 1.19 and an ovariectomized rat model were used, respectively. Expression levels of genes and proteins related to osteoblast differentiation and mineralization were measured by real‐time polymerase chain reaction (qPCR) and Western blotting, respectively. Alizarin red S staining was performed to measure osteoblast miner-alization. Heat‐killed MJ2 (hkMJ2)‐treated cells showed significantly increased osteoblast differentiation via an increase in the osteoprotegerin (OPG)/receptor activator of nuclear factor‐κB ligand (RANKL) ratio and significantly increased osteoblast mineralization by stimulating the expression of bone morphogenetic protein 2 and runt‐related transcription factor 2. Additionally, oral admin-istration of live or heat‐killed MJ2 to ovariectomized rats inhibited osteoporosis‐induced bone loss. Specifically, surface proteins isolated from MJ2 promoted osteoblast differentiation and minerali-zation. In conclusion, MJ2 enhanced osteoblast differentiation and mineralization through the OPG/RANKL signaling pathway and the effective component of MJ2 might be its surface proteins.
AB - Osteoblast differentiation is important for the development of bone and the maintenance of bone density. Propionibacterium freudenreichii is a probiotic with an anti‐inflammatory property. The aim of this study was to investigate the enhancement effect of P. freudenreichii MJ2 (MJ2) isolated from raw milk on osteoblast differentiation, mineralization, and its signaling pathway. For in vitro and in vivo experiments, human fetal osteoblastic cell line hFOB 1.19 and an ovariectomized rat model were used, respectively. Expression levels of genes and proteins related to osteoblast differentiation and mineralization were measured by real‐time polymerase chain reaction (qPCR) and Western blotting, respectively. Alizarin red S staining was performed to measure osteoblast miner-alization. Heat‐killed MJ2 (hkMJ2)‐treated cells showed significantly increased osteoblast differentiation via an increase in the osteoprotegerin (OPG)/receptor activator of nuclear factor‐κB ligand (RANKL) ratio and significantly increased osteoblast mineralization by stimulating the expression of bone morphogenetic protein 2 and runt‐related transcription factor 2. Additionally, oral admin-istration of live or heat‐killed MJ2 to ovariectomized rats inhibited osteoporosis‐induced bone loss. Specifically, surface proteins isolated from MJ2 promoted osteoblast differentiation and minerali-zation. In conclusion, MJ2 enhanced osteoblast differentiation and mineralization through the OPG/RANKL signaling pathway and the effective component of MJ2 might be its surface proteins.
KW - Osteoblast mineralization
KW - Osteoprotegerin
KW - Propionibacterium freudenreichii
KW - Receptor activator of nuclear factor‐κB ligand
KW - Surface proteins
UR - http://www.scopus.com/inward/record.url?scp=85102947207&partnerID=8YFLogxK
U2 - 10.3390/microorganisms9040673
DO - 10.3390/microorganisms9040673
M3 - Article
AN - SCOPUS:85102947207
VL - 9
JO - Microorganisms
JF - Microorganisms
SN - 2076-2607
IS - 4
M1 - 673
ER -