Probiotics inhibit lipopolysaccharide-induced interleukin-8 secretion from intestinal epithelial cells

Hyun Wook Oh, Gi Hoon Jeun, Jin Lee, Tae Hoon Chun, Sae Hun Kim

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


It has been suggested that probiotics could be useful for the prevention of symptomatic relapse in patients with inflammatory bowel disease (IBD). Interleukin (IL)-8 has been well recognized as one of the pro-inflammatory cytokines that could trigger inflammation and epithelial barrier dysfunction. In this study, the anti-inflammatory effects of probiotics were investigated using a human epithelial cell line (HT-29). Probiotics from infant feces and kimchi were tested for their cytotoxicity and effects on adhesion to epithelial cells. The present results show that seven strains could form 70 % adhesion on HT-29. The probiotics used in this study did not affect HT-29 cell viability. To screen anti-inflammatory lactic acid bacteria, HT-29 cells were pretreated with live and heat-killed probiotics, and lipopolysaccharide (LPS) (1 ?g/mL) was then added to stimulate the cells. The cell culture supernatant was then used to measure IL-8 secretion by ELISA, and the cell pellet was used to determine IL-8 and toll-like receptor (TLR-4) mRNA expression levels by RT-PCR. Some probiotics (KJP421, KDK411, SRK414, E4191, KY21, and KY210) exhibited anti-inflammatory effects through the repression of IL-8 secretion from HT- 29 cells. In particular, Lactobacillus salivarius E4191, originating from Egyptian infant feces, not only decreased IL-8 mRNA expression, but also decreased TLR-4 expression. These results indicate that Lactobacillus salivarius E4191 may have a protective effect in intestinal epithelial cells.

Original languageEnglish
Pages (from-to)434-440
Number of pages7
JournalKorean Journal for Food Science of Animal Resources
Issue number4
Publication statusPublished - 2012 Aug


  • Anti-inflammatory
  • Inflammatory bowel disease
  • Interleukin-8
  • Probiotics

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology


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