Abstract
It has been suggested that probiotics could be useful for the prevention of symptomatic relapse in patients with inflammatory bowel disease (IBD). Interleukin (IL)-8 has been well recognized as one of the pro-inflammatory cytokines that could trigger inflammation and epithelial barrier dysfunction. In this study, the anti-inflammatory effects of probiotics were investigated using a human epithelial cell line (HT-29). Probiotics from infant feces and kimchi were tested for their cytotoxicity and effects on adhesion to epithelial cells. The present results show that seven strains could form 70 % adhesion on HT-29. The probiotics used in this study did not affect HT-29 cell viability. To screen anti-inflammatory lactic acid bacteria, HT-29 cells were pretreated with live and heat-killed probiotics, and lipopolysaccharide (LPS) (1 ?g/mL) was then added to stimulate the cells. The cell culture supernatant was then used to measure IL-8 secretion by ELISA, and the cell pellet was used to determine IL-8 and toll-like receptor (TLR-4) mRNA expression levels by RT-PCR. Some probiotics (KJP421, KDK411, SRK414, E4191, KY21, and KY210) exhibited anti-inflammatory effects through the repression of IL-8 secretion from HT- 29 cells. In particular, Lactobacillus salivarius E4191, originating from Egyptian infant feces, not only decreased IL-8 mRNA expression, but also decreased TLR-4 expression. These results indicate that Lactobacillus salivarius E4191 may have a protective effect in intestinal epithelial cells.
| Original language | English |
|---|---|
| Pages (from-to) | 434-440 |
| Number of pages | 7 |
| Journal | Korean Journal for Food Science of Animal Resources |
| Volume | 32 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2012 Aug 1 |
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Keywords
- Anti-inflammatory
- Inflammatory bowel disease
- Interleukin-8
- Probiotics
ASJC Scopus subject areas
- Food Science
- Animal Science and Zoology
Cite this
Probiotics inhibit lipopolysaccharide-induced interleukin-8 secretion from intestinal epithelial cells. / Oh, Hyun Wook; Jeun, Gi Hoon; Lee, Jin; Chun, Taehoon; Kim, Sae Hun.
In: Korean Journal for Food Science of Animal Resources, Vol. 32, No. 4, 01.08.2012, p. 434-440.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Probiotics inhibit lipopolysaccharide-induced interleukin-8 secretion from intestinal epithelial cells
AU - Oh, Hyun Wook
AU - Jeun, Gi Hoon
AU - Lee, Jin
AU - Chun, Taehoon
AU - Kim, Sae Hun
PY - 2012/8/1
Y1 - 2012/8/1
N2 - It has been suggested that probiotics could be useful for the prevention of symptomatic relapse in patients with inflammatory bowel disease (IBD). Interleukin (IL)-8 has been well recognized as one of the pro-inflammatory cytokines that could trigger inflammation and epithelial barrier dysfunction. In this study, the anti-inflammatory effects of probiotics were investigated using a human epithelial cell line (HT-29). Probiotics from infant feces and kimchi were tested for their cytotoxicity and effects on adhesion to epithelial cells. The present results show that seven strains could form 70 % adhesion on HT-29. The probiotics used in this study did not affect HT-29 cell viability. To screen anti-inflammatory lactic acid bacteria, HT-29 cells were pretreated with live and heat-killed probiotics, and lipopolysaccharide (LPS) (1 ?g/mL) was then added to stimulate the cells. The cell culture supernatant was then used to measure IL-8 secretion by ELISA, and the cell pellet was used to determine IL-8 and toll-like receptor (TLR-4) mRNA expression levels by RT-PCR. Some probiotics (KJP421, KDK411, SRK414, E4191, KY21, and KY210) exhibited anti-inflammatory effects through the repression of IL-8 secretion from HT- 29 cells. In particular, Lactobacillus salivarius E4191, originating from Egyptian infant feces, not only decreased IL-8 mRNA expression, but also decreased TLR-4 expression. These results indicate that Lactobacillus salivarius E4191 may have a protective effect in intestinal epithelial cells.
AB - It has been suggested that probiotics could be useful for the prevention of symptomatic relapse in patients with inflammatory bowel disease (IBD). Interleukin (IL)-8 has been well recognized as one of the pro-inflammatory cytokines that could trigger inflammation and epithelial barrier dysfunction. In this study, the anti-inflammatory effects of probiotics were investigated using a human epithelial cell line (HT-29). Probiotics from infant feces and kimchi were tested for their cytotoxicity and effects on adhesion to epithelial cells. The present results show that seven strains could form 70 % adhesion on HT-29. The probiotics used in this study did not affect HT-29 cell viability. To screen anti-inflammatory lactic acid bacteria, HT-29 cells were pretreated with live and heat-killed probiotics, and lipopolysaccharide (LPS) (1 ?g/mL) was then added to stimulate the cells. The cell culture supernatant was then used to measure IL-8 secretion by ELISA, and the cell pellet was used to determine IL-8 and toll-like receptor (TLR-4) mRNA expression levels by RT-PCR. Some probiotics (KJP421, KDK411, SRK414, E4191, KY21, and KY210) exhibited anti-inflammatory effects through the repression of IL-8 secretion from HT- 29 cells. In particular, Lactobacillus salivarius E4191, originating from Egyptian infant feces, not only decreased IL-8 mRNA expression, but also decreased TLR-4 expression. These results indicate that Lactobacillus salivarius E4191 may have a protective effect in intestinal epithelial cells.
KW - Anti-inflammatory
KW - Inflammatory bowel disease
KW - Interleukin-8
KW - Probiotics
UR - http://www.scopus.com/inward/record.url?scp=84871292067&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84871292067&partnerID=8YFLogxK
U2 - 10.5851/kosfa.2012.32.4.434
DO - 10.5851/kosfa.2012.32.4.434
M3 - Article
VL - 32
SP - 434
EP - 440
JO - Korean Journal for Food Science of Animal Resources
JF - Korean Journal for Food Science of Animal Resources
SN - 1225-8563
IS - 4
ER -