Process strategy for 2,3-butanediol production in fed-batch culture by acetate addition

Sang Jun Lee, Han Suk Choi, Chan Kyum Kim, Laxmi Prasad Thapa, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Various strategies were studied to enhance 2,3-butanediol production using Enterobacter aerogenes SUMI014. The synergistic effect of acetate improved yield and productivity, and resulted in 32.3% increase in 2,3-butanediol production, compared to the wild strain. Optimizing the fermentation conditions successfully increased the 2,3-butanediol production. In batch fermentation, 93.75. g/L of 2,3-butanediol was obtained within 54. h, along with 0.49. g/g of yield and 1.74. g/L/h of productivity. The highest 2,3-butanediol production achieved was in fed-batch fermentation with acetate addition strategy, with production, yield and productivity of 126.10. g/L, 0.38. g/g and 2.10. g/L/h, respectively.

Original languageEnglish
JournalJournal of Industrial and Engineering Chemistry
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Acetates
Fermentation
Productivity
2,3-butylene glycol

Keywords

  • 2,3-Butanediol
  • Acetate supplementation
  • Enterobacter aerogenes
  • Fermentation
  • Lactate dehydrogenase

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Process strategy for 2,3-butanediol production in fed-batch culture by acetate addition. / Lee, Sang Jun; Choi, Han Suk; Kim, Chan Kyum; Thapa, Laxmi Prasad; Park, Chulhwan; Kim, Seung Wook.

In: Journal of Industrial and Engineering Chemistry, 2017.

Research output: Contribution to journalArticle

Lee, Sang Jun ; Choi, Han Suk ; Kim, Chan Kyum ; Thapa, Laxmi Prasad ; Park, Chulhwan ; Kim, Seung Wook. / Process strategy for 2,3-butanediol production in fed-batch culture by acetate addition. In: Journal of Industrial and Engineering Chemistry. 2017.
@article{dcc9ecb3fc784d28b9a447a391f4bd72,
title = "Process strategy for 2,3-butanediol production in fed-batch culture by acetate addition",
abstract = "Various strategies were studied to enhance 2,3-butanediol production using Enterobacter aerogenes SUMI014. The synergistic effect of acetate improved yield and productivity, and resulted in 32.3{\%} increase in 2,3-butanediol production, compared to the wild strain. Optimizing the fermentation conditions successfully increased the 2,3-butanediol production. In batch fermentation, 93.75. g/L of 2,3-butanediol was obtained within 54. h, along with 0.49. g/g of yield and 1.74. g/L/h of productivity. The highest 2,3-butanediol production achieved was in fed-batch fermentation with acetate addition strategy, with production, yield and productivity of 126.10. g/L, 0.38. g/g and 2.10. g/L/h, respectively.",
keywords = "2,3-Butanediol, Acetate supplementation, Enterobacter aerogenes, Fermentation, Lactate dehydrogenase",
author = "Lee, {Sang Jun} and Choi, {Han Suk} and Kim, {Chan Kyum} and Thapa, {Laxmi Prasad} and Chulhwan Park and Kim, {Seung Wook}",
year = "2017",
doi = "10.1016/j.jiec.2017.07.008",
language = "English",
journal = "Journal of Industrial and Engineering Chemistry",
issn = "1226-086X",
publisher = "Korean Society of Industrial Engineering Chemistry",

}

TY - JOUR

T1 - Process strategy for 2,3-butanediol production in fed-batch culture by acetate addition

AU - Lee, Sang Jun

AU - Choi, Han Suk

AU - Kim, Chan Kyum

AU - Thapa, Laxmi Prasad

AU - Park, Chulhwan

AU - Kim, Seung Wook

PY - 2017

Y1 - 2017

N2 - Various strategies were studied to enhance 2,3-butanediol production using Enterobacter aerogenes SUMI014. The synergistic effect of acetate improved yield and productivity, and resulted in 32.3% increase in 2,3-butanediol production, compared to the wild strain. Optimizing the fermentation conditions successfully increased the 2,3-butanediol production. In batch fermentation, 93.75. g/L of 2,3-butanediol was obtained within 54. h, along with 0.49. g/g of yield and 1.74. g/L/h of productivity. The highest 2,3-butanediol production achieved was in fed-batch fermentation with acetate addition strategy, with production, yield and productivity of 126.10. g/L, 0.38. g/g and 2.10. g/L/h, respectively.

AB - Various strategies were studied to enhance 2,3-butanediol production using Enterobacter aerogenes SUMI014. The synergistic effect of acetate improved yield and productivity, and resulted in 32.3% increase in 2,3-butanediol production, compared to the wild strain. Optimizing the fermentation conditions successfully increased the 2,3-butanediol production. In batch fermentation, 93.75. g/L of 2,3-butanediol was obtained within 54. h, along with 0.49. g/g of yield and 1.74. g/L/h of productivity. The highest 2,3-butanediol production achieved was in fed-batch fermentation with acetate addition strategy, with production, yield and productivity of 126.10. g/L, 0.38. g/g and 2.10. g/L/h, respectively.

KW - 2,3-Butanediol

KW - Acetate supplementation

KW - Enterobacter aerogenes

KW - Fermentation

KW - Lactate dehydrogenase

UR - http://www.scopus.com/inward/record.url?scp=85025803252&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85025803252&partnerID=8YFLogxK

U2 - 10.1016/j.jiec.2017.07.008

DO - 10.1016/j.jiec.2017.07.008

M3 - Article

AN - SCOPUS:85025803252

JO - Journal of Industrial and Engineering Chemistry

JF - Journal of Industrial and Engineering Chemistry

SN - 1226-086X

ER -