TY - JOUR
T1 - Process strategy for 2,3-butanediol production in fed-batch culture by acetate addition
AU - Lee, Sang Jun
AU - Choi, Han Suk
AU - Kim, Chan Kyum
AU - Thapa, Laxmi Prasad
AU - Park, Chulhwan
AU - Kim, Seung Wook
N1 - Funding Information:
This study was supported by BK21 Plus program and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education ( NRF-2016R1D1A1A09918327 ).
Publisher Copyright:
© 2017 The Korean Society of Industrial and Engineering Chemistry
PY - 2017/12/25
Y1 - 2017/12/25
N2 - Various strategies were studied to enhance 2,3-butanediol production using Enterobacter aerogenes SUMI014. The synergistic effect of acetate improved yield and productivity, and resulted in 32.3% increase in 2,3-butanediol production, compared to the wild strain. Optimizing the fermentation conditions successfully increased the 2,3-butanediol production. In batch fermentation, 93.75 g/L of 2,3-butanediol was obtained within 54 h, along with 0.49 g/g of yield and 1.74 g/L/h of productivity. The highest 2,3-butanediol production achieved was in fed-batch fermentation with acetate addition strategy, with production, yield and productivity of 126.10 g/L, 0.38 g/g and 2.10 g/L/h, respectively.
AB - Various strategies were studied to enhance 2,3-butanediol production using Enterobacter aerogenes SUMI014. The synergistic effect of acetate improved yield and productivity, and resulted in 32.3% increase in 2,3-butanediol production, compared to the wild strain. Optimizing the fermentation conditions successfully increased the 2,3-butanediol production. In batch fermentation, 93.75 g/L of 2,3-butanediol was obtained within 54 h, along with 0.49 g/g of yield and 1.74 g/L/h of productivity. The highest 2,3-butanediol production achieved was in fed-batch fermentation with acetate addition strategy, with production, yield and productivity of 126.10 g/L, 0.38 g/g and 2.10 g/L/h, respectively.
KW - 2,3-Butanediol
KW - Acetate supplementation
KW - Enterobacter aerogenes
KW - Fermentation
KW - Lactate dehydrogenase
UR - http://www.scopus.com/inward/record.url?scp=85025803252&partnerID=8YFLogxK
U2 - 10.1016/j.jiec.2017.07.008
DO - 10.1016/j.jiec.2017.07.008
M3 - Article
AN - SCOPUS:85025803252
VL - 56
SP - 157
EP - 162
JO - Journal of Industrial and Engineering Chemistry
JF - Journal of Industrial and Engineering Chemistry
SN - 1226-086X
ER -