Production and characterization of cellobiose dehydrogenase from Phanerochaete chrysosporium KCCM 60256 and its application for an enzymatic fuel cell

Han Suk Choi, Dong Sup Kim, Laxmi Prasad Thapa, Sang Jun Lee, Sung Bong Kim, Jaehoon Cho, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The enzyme cellobiose dehydrogenase (CDH), with high ability of electron transport, has been widely used in enzymatic fuel cells or biosensors. In this study, the cellobiose dehydrogenase gene from Phanerochaete chrysosporium KCCM 60256 was amplified and expressed in the methylotrophic yeast Pichia pastoris X-33. The recombinant enzyme (PcCDH) was purified using a metal affinity chromatography under non-denaturing conditions. The purified enzyme was analyzed by SDS-PAGE, confirming a corresponding band about 100 kDa. The enzyme activity of this purified PcCDH was determined as 1,845U/L (65mg/L protein). The enzyme showed the maximum activity at pH 4.5 and high activity in broad ranges of temperature from 30°C to 60°C. Moreover, the application of PcCDH to enzymatic fuel cell (EFC) was demonstrated. Lactose was used as the substrate in the EFC system; anode and cathode were immobilized with PcCDH and laccase, respectively. The cell’s open circuit voltage and maximum power density of the EFC system were, respectively, determined as 0.435 V and 314 μW/cm2 (at 0.247 V) with 10 mM lactose.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalKorean Journal of Chemical Engineering
DOIs
Publication statusAccepted/In press - 2016 Sep 3

Fingerprint

cellobiose-quinone oxidoreductase
Enzymatic fuel cells
Enzymes
Lactose
Affinity chromatography
Laccase
Enzyme activity
Open circuit voltage
Biosensors
Yeast
Anodes
Cathodes
Genes
Metals
Proteins
Oxidoreductases
Substrates

Keywords

  • Cellobiose Dehydrogenase
  • Enzymatic Fuel Cell
  • Phanerochaete chrysosporium
  • Pichia pastoris

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Production and characterization of cellobiose dehydrogenase from Phanerochaete chrysosporium KCCM 60256 and its application for an enzymatic fuel cell. / Choi, Han Suk; Kim, Dong Sup; Thapa, Laxmi Prasad; Lee, Sang Jun; Kim, Sung Bong; Cho, Jaehoon; Park, Chulhwan; Kim, Seung Wook.

In: Korean Journal of Chemical Engineering, 03.09.2016, p. 1-8.

Research output: Contribution to journalArticle

Choi, Han Suk ; Kim, Dong Sup ; Thapa, Laxmi Prasad ; Lee, Sang Jun ; Kim, Sung Bong ; Cho, Jaehoon ; Park, Chulhwan ; Kim, Seung Wook. / Production and characterization of cellobiose dehydrogenase from Phanerochaete chrysosporium KCCM 60256 and its application for an enzymatic fuel cell. In: Korean Journal of Chemical Engineering. 2016 ; pp. 1-8.
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