Production and stability of chlorine dioxide in organic acid solutions as affected by pH, type of acid, and concentration of sodium chlorite, and its effectiveness in inactivating Bacillus cereus spores

Hoikyung Kim, Youngjee Kang, Larry R. Beuchat, Jee-Hoon Ryu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We studied the production and stability of chlorine dioxide (ClO 2) in organic acid solutions and its effectiveness in killing Bacillus cereus spores. Sodium chlorite (5000, 10,000, or 50,000 μg/ml) was added to 5% acetic, citric, or lactic acid solution, adjusted to pH 3.0, 4.0, 5.0, or 6.0, and held at 21 °C for up to 14 days. The amount of ClO 2 produced was higher as the concentration of sodium chlorite was increased and as the pH of the acid solutions was decreased. However, the stability in production of ClO 2 was enhanced by increasing the pH of the organic acid solutions. To evaluate the lethal activity of ClO 2 produced in various acid solutions as affected by acidulant and pH, suspensions of B. cereus spores were treated at 21 °C for 1, 3, 5, or 10 min in hydrochloric acid or organic acid solutions (pH 3.0, 4.0, 5.0, or 6.0) containing ClO 2 at concentrations of 100, 50, or 25 μg/ml. Populations of viable spores treated with ClO 2 at concentrations of 100 or 50 μg/ml in organic acid solutions decreased more rapidly than populations treated with the same concentrations of ClO 2 in HCl. Rates of inactivation tended to increase with higher pH of ClO 2 solutions. Results show that ClO 2 formed in organic acid solutions has higher stability and is more lethal to B. cereus spores than ClO 2 formed at the same concentration in HCl solution. This finding emphasizes the benefits of using organic acid solutions to prepare ClO 2 intended for use as an antimicrobial.

Original languageEnglish
Pages (from-to)964-969
Number of pages6
JournalFood Microbiology
Volume25
Issue number8
DOIs
Publication statusPublished - 2008 Dec 1

Fingerprint

chlorites
chlorine dioxide
Bacillus cereus
Spores
organic acids and salts
spores
sodium
Acids
acids
chlorite
acidulants
Hydrochloric Acid
hydrochloric acid
Citric Acid
citric acid
Acetic Acid
lactic acid
Population
Lactic Acid
Suspensions

Keywords

  • Bacillus cereus
  • Chlorine dioxide
  • Organic acid

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

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title = "Production and stability of chlorine dioxide in organic acid solutions as affected by pH, type of acid, and concentration of sodium chlorite, and its effectiveness in inactivating Bacillus cereus spores",
abstract = "We studied the production and stability of chlorine dioxide (ClO 2) in organic acid solutions and its effectiveness in killing Bacillus cereus spores. Sodium chlorite (5000, 10,000, or 50,000 μg/ml) was added to 5{\%} acetic, citric, or lactic acid solution, adjusted to pH 3.0, 4.0, 5.0, or 6.0, and held at 21 °C for up to 14 days. The amount of ClO 2 produced was higher as the concentration of sodium chlorite was increased and as the pH of the acid solutions was decreased. However, the stability in production of ClO 2 was enhanced by increasing the pH of the organic acid solutions. To evaluate the lethal activity of ClO 2 produced in various acid solutions as affected by acidulant and pH, suspensions of B. cereus spores were treated at 21 °C for 1, 3, 5, or 10 min in hydrochloric acid or organic acid solutions (pH 3.0, 4.0, 5.0, or 6.0) containing ClO 2 at concentrations of 100, 50, or 25 μg/ml. Populations of viable spores treated with ClO 2 at concentrations of 100 or 50 μg/ml in organic acid solutions decreased more rapidly than populations treated with the same concentrations of ClO 2 in HCl. Rates of inactivation tended to increase with higher pH of ClO 2 solutions. Results show that ClO 2 formed in organic acid solutions has higher stability and is more lethal to B. cereus spores than ClO 2 formed at the same concentration in HCl solution. This finding emphasizes the benefits of using organic acid solutions to prepare ClO 2 intended for use as an antimicrobial.",
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author = "Hoikyung Kim and Youngjee Kang and Beuchat, {Larry R.} and Jee-Hoon Ryu",
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AU - Kim, Hoikyung

AU - Kang, Youngjee

AU - Beuchat, Larry R.

AU - Ryu, Jee-Hoon

PY - 2008/12/1

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N2 - We studied the production and stability of chlorine dioxide (ClO 2) in organic acid solutions and its effectiveness in killing Bacillus cereus spores. Sodium chlorite (5000, 10,000, or 50,000 μg/ml) was added to 5% acetic, citric, or lactic acid solution, adjusted to pH 3.0, 4.0, 5.0, or 6.0, and held at 21 °C for up to 14 days. The amount of ClO 2 produced was higher as the concentration of sodium chlorite was increased and as the pH of the acid solutions was decreased. However, the stability in production of ClO 2 was enhanced by increasing the pH of the organic acid solutions. To evaluate the lethal activity of ClO 2 produced in various acid solutions as affected by acidulant and pH, suspensions of B. cereus spores were treated at 21 °C for 1, 3, 5, or 10 min in hydrochloric acid or organic acid solutions (pH 3.0, 4.0, 5.0, or 6.0) containing ClO 2 at concentrations of 100, 50, or 25 μg/ml. Populations of viable spores treated with ClO 2 at concentrations of 100 or 50 μg/ml in organic acid solutions decreased more rapidly than populations treated with the same concentrations of ClO 2 in HCl. Rates of inactivation tended to increase with higher pH of ClO 2 solutions. Results show that ClO 2 formed in organic acid solutions has higher stability and is more lethal to B. cereus spores than ClO 2 formed at the same concentration in HCl solution. This finding emphasizes the benefits of using organic acid solutions to prepare ClO 2 intended for use as an antimicrobial.

AB - We studied the production and stability of chlorine dioxide (ClO 2) in organic acid solutions and its effectiveness in killing Bacillus cereus spores. Sodium chlorite (5000, 10,000, or 50,000 μg/ml) was added to 5% acetic, citric, or lactic acid solution, adjusted to pH 3.0, 4.0, 5.0, or 6.0, and held at 21 °C for up to 14 days. The amount of ClO 2 produced was higher as the concentration of sodium chlorite was increased and as the pH of the acid solutions was decreased. However, the stability in production of ClO 2 was enhanced by increasing the pH of the organic acid solutions. To evaluate the lethal activity of ClO 2 produced in various acid solutions as affected by acidulant and pH, suspensions of B. cereus spores were treated at 21 °C for 1, 3, 5, or 10 min in hydrochloric acid or organic acid solutions (pH 3.0, 4.0, 5.0, or 6.0) containing ClO 2 at concentrations of 100, 50, or 25 μg/ml. Populations of viable spores treated with ClO 2 at concentrations of 100 or 50 μg/ml in organic acid solutions decreased more rapidly than populations treated with the same concentrations of ClO 2 in HCl. Rates of inactivation tended to increase with higher pH of ClO 2 solutions. Results show that ClO 2 formed in organic acid solutions has higher stability and is more lethal to B. cereus spores than ClO 2 formed at the same concentration in HCl solution. This finding emphasizes the benefits of using organic acid solutions to prepare ClO 2 intended for use as an antimicrobial.

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