Production of 1,2-propanediol from glycerol in saccharomyces cerevisiae

Joon Young Jung, Hyun Shik Yun, Jinwon Lee, Min-Kyu Oh

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Glycerol has become an attractive carbon source in the biotechnology industry owing to its low price and reduced state. However, glycerol is rarely used as a carbon source in Saccharomyces cerevisiae because of its low utilization rate. In this study, we used glycerol as a main carbon source in S. cerevisiae to produce 1,2-propanediol. Metabolically engineered S. cerevisiae strains with overexpression of glycerol dissimilation pathway genes, including glycerol kinase (GUT1), glycerol 3-phosphate dehydrogenase (GUT2), glycerol dehydrogenase (gdh), and a glycerol transporter gene (GUP1), showed increased glycerol utilization and growth rate. More significant improvement of glycerol utilization and growth rate was accomplished by introducing 1,2-propanediol pathway genes, mgs (methylglyoxal synthase) and gldA (glycerol dehydrogenase) from Escherichia coli. By engineering both glycerol dissimilation and 1,2-propanediol pathways, the glycerol utilization and growth rate were improved 141% and 77%, respectively, and a 2.19 g 1,2-propanediol/l titer was achieved in 1% (v/v) glycerolcontaining YEPD medium in engineered S. cerevisiae.

Original languageEnglish
Pages (from-to)846-853
Number of pages8
JournalJournal of Microbiology and Biotechnology
Volume21
Issue number8
DOIs
Publication statusPublished - 2011 Aug 1

Keywords

  • 1,2-propanediol
  • Glycerol
  • Metabolic engineering
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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